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High level soluble expression and one-step purification of IBDV VP2 protein in Escherichia coli

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Abstract

Objectives

To improve the expression of soluble IBDV VP2 protein by using different tagged vectors in Escherichia coli.

Results

Fusion tags, Grifin, MBP, SUMO, thioredoxin, γ-crystallin, ArsC and PpiB, enhanced the expression and solubility of VP2 protein. The fusion proteins were purified by Ni–NTA chromatography, MBP-VP2 showed the highest purity about 90 %. After removing the MBP tag, VP2 self-assembled into virus-like particles, ~25 nm diam. Results from AGP suggested the recombinant IBDV VP2 protein identified by reference serum like IBDV.

Conclusion

All the seven tags enhanced the expression and solubility of IBDV VP2 protein. The recombinant protein self-assembly into virus like particles and possess antigenicity as reference IBDV.

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Acknowledgments

This work study was supported by the grant from program of the National Nature Science Foundation of China (No.31472177), and Major Program of Science and Technology in Henan (141100110100), and Henan Province Scientific and technological innovation talent program in Colleges (14HASTIT027), and the Key Scientific and Technological Project of Henan Province, China (152102110128).

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Authors and Affiliations

  1. College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou, 450002, People’s Republic of China

    Dawei Jiang, Gaiping Zhang, Guoyu Yang, Yapeng Song, Guoqiang Wang, Jucai Wang & Baolei Zhao

  2. Henan Provincial Key Laboratory of Animal Immunology, Henan Academy of Agricultural Sciences, Zhengzhou, 450002, People’s Republic of China

    Dawei Jiang, Yunchao Liu, Gaiping Zhang, Yumei Chen, Pengchao Ji, Chang Liu, Yapeng Song, Yunfang Su, Guoqiang Wang, Jucai Wang, Baolei Zhao & Ruiguang Deng

  3. School of Life Sciences, Zhengzhou University, Zhengzhou, 450001, People’s Republic of China

    Aiping Wang & Yumei Chen

Authors
  1. Dawei Jiang
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  2. Yunchao Liu
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  3. Aiping Wang
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  8. Chang Liu
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  9. Yapeng Song
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  10. Yunfang Su
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  11. Guoqiang Wang
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  12. Jucai Wang
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  13. Baolei Zhao
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  14. Ruiguang Deng
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Corresponding author

Correspondence to Gaiping Zhang.

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Jiang, D., Liu, Y., Wang, A. et al. High level soluble expression and one-step purification of IBDV VP2 protein in Escherichia coli . Biotechnol Lett 38, 901–908 (2016). https://doi.org/10.1007/s10529-016-2073-8

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  • DOI: https://doi.org/10.1007/s10529-016-2073-8

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