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Heterotrophic nitrification and aerobic denitrification by a novel Halomonas campisalis

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Abstract

A novel halophilic strain that could carry out heterotrophic nitrification and aerobic denitrification was isolated and named as Halomonas campisalis ha3. It removed inorganic nitrogen compounds (e.g. NO3 −, NO2 − and NH4 +) simultaneously, and grew well in the medium containing up to 20 % (w/v) NaCl. PCR revealed four genes in the genome of ha3 related to aerobic denitrification: napA, nirS, norB and nosZ. The optimal conditions for aerobic denitrification were pH 9.0, at 37 °C, with 4 % (w/v) NaCl and sodium succinate as carbon source. The nitrogen removal rate was 87.5 mg NO3 −–N l−1 h−1. Therefore, this strain is a potential aerobic denitrifier for the treatment of saline wastewater.

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Acknowledgments

This study was financially supported by the National Natural Science Foundation of China (Grant Nos. 21207060 and 21076216). We are also grateful to Dr. Yanhe Ma from the Institute of Microbiology, Chinese Academy of Sciences, for the supply of sludge samples from saline-alkali lake.

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Authors and Affiliations

  1. National Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Zhongguancun Bei-er-tiao 1, Haidian District, P.O. Box 353, Beijing, 100190, People’s Republic of China

    Yan Guo, Caixia Wang, Jianfei Liu, Daojiang Yan, Yilan Liu & Jianmin Xing

  2. University of Chinese Academy of Sciences, Beijing, 100049, People’s Republic of China

    Yan Guo, Ke Li, Caixia Wang, Jianfei Liu, Daojiang Yan & Yilan Liu

  3. 101 Institute of the Ministry of Civil Affairs, Beijing, 100070, People’s Republic of China

    Xuemei Zhou, Yuguang Li & Dehui Yang

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  1. Yan Guo
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  2. Xuemei Zhou
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  3. Yuguang Li
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  4. Ke Li
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  5. Caixia Wang
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  6. Jianfei Liu
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  7. Daojiang Yan
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  8. Yilan Liu
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  9. Dehui Yang
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  10. Jianmin Xing
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Correspondence to Jianmin Xing.

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Guo, Y., Zhou, X., Li, Y. et al. Heterotrophic nitrification and aerobic denitrification by a novel Halomonas campisalis . Biotechnol Lett 35, 2045–2049 (2013). https://doi.org/10.1007/s10529-013-1294-3

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  • DOI: https://doi.org/10.1007/s10529-013-1294-3

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