Abstract
Hairy root cultures of Gynostemma pentaphyllum were established by infecting leaf discs with Agrobacterium rhizogenes. The dry biomass of hairy roots grown in MS medium for 49 days was 7.3 g l−1 with a gypenoside content of 38 mg g−1 dry wt.
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References
CO Akalezi S Liu QS Li JT Yu JJ Zhong (1999) ArticleTitleCombined effects of initial sucrose concentration and inoculum size on cell growth and ginseng saponin production by suspension cultures of Panax ginseng Process Biochem 34 639–642 Occurrence Handle10.1016/S0032-9592(98)00132-0
R Bhadra JV Shanks (1997) ArticleTitleTransient studies of nutrient uptake, growth, and indole alkaloid accumulation in heterotrophic cultures of hairy roots of Catharanthus roseus Biotechnol. Bioeng 55 527–534 Occurrence Handle10.1002/(SICI)1097-0290(19970805)55:3<527::AID-BIT9>3.0.CO;2-E
HM Fei KF Mei X Shen YM Ye ZP Lin LH Peng (1993) ArticleTitleTransformation of Gynostemma pentaphyllum by Agrobacterium rhizogenes and saponin production in hairy root cultures Acta Bot. Sin 35 626–631
OL Gamborg RA Miller K Ojima (1968) ArticleTitleNutrient requirements of suspension cultures of soybean root cells Exp. Cell Res 50 151–158 Occurrence Handle10.1016/0014-4827(68)90403-5 Occurrence Handle5650857
TSC Li G Mazza AC Cottrell L Gao (1996) ArticleTitleGinsenosides in roots and leaves of American ginseng J. Agric. Food Chem 44 717–720 Occurrence Handle10.1021/jf950309f
SB Liu R Lin ZH Hu (2005) ArticleTitleHistochemical localization of ginsenosides in Gynostemma pentaphyllum and the content changes of total gypenosides Acta Biol. Exp. Sin 38 54–60
T Murashige F Skoog (1962) ArticleTitleA revised medium for rapid growth and bioassays with tobacco tissue cultures Physiol. Plant 15 473–497
Å Norberg NK Hoa E Liepinsh DV Phan ND Thuan H Jörnvall R Sillard C-G Östenson (2004) ArticleTitleA novel insulin-releasing substance, phanoside, from the plant Gynostemma pentaphyllum J. Biol. Chem 279 41361–41367 Occurrence Handle10.1074/jbc.M403435200 Occurrence Handle15220351
GF Payne V Bringi C Prince ML Shuler (1992) Plant Cell and Tissue Culture in Liquid Systems Hanser Publishing New York
N Sevón K-M Oksman-Caldentey (2002) ArticleTitleAgrobacterium rhizogenes-mediated transformation: Root cultures as a source of alkaloids Planta Med 68 859–868 Occurrence Handle10.1055/s-2002-34924 Occurrence Handle12391546
MA Subroto PM Doran (1994) ArticleTitleProduction of steroidal alkaloids by hairy roots of Solanum aviculare and the effect of gibberellic acid Plant Cell Tiss. Org. Cult 38 93–102 Occurrence Handle10.1007/BF00033866
D Thomson R Henry (1995) ArticleTitleSingle-step protocol for preparation of plant tissue for analysis by PCR BioTechniques 19 394–400 Occurrence Handle7495552
G Vervliet M Holsters H Teuchy M Montagu Particlevan J Schell (1975) ArticleTitleCharacterization of different plaque forming and defective temperate phages in Agrobacterium strains J. Gen. Virol 26 33–48 Occurrence Handle1123610
PDG Wilson MG Hilton RJ Robins MJC Rhodes (1987) Fermentation studies of transformed root cultures GW, Moody PB Baker (Eds) Bioreactors and Biotransformation Elsevier Press London 38–51
F Yin LH Hu RX Pan (2004) ArticleTitleNovel dammarane-type glycosides from Gynostemma pentaphyllum Chem. Pharm. Bull 52 1440–1444 Occurrence Handle10.1248/cpb.52.1440 Occurrence Handle15577241
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Chang, CK., Chang, KS., Lin, YC. et al. Hairy Root Cultures of Gynostemma pentaphyllum (Thunb.) Makino: A Promising Approach for the Production of Gypenosides as an Alternative of Ginseng Saponins. Biotechnol Lett 27, 1165–1169 (2005). https://doi.org/10.1007/s10529-005-8653-7
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DOI: https://doi.org/10.1007/s10529-005-8653-7