References
Bagis H, Papuccuoglu S (1997) Studies on the production of transgenic mice. Turk J Vet Anim Sci 21:287–292
Bagis H, Odaman H, Sağırkaya, Dinyéss A (2002) Production of transgenic mice from vitrified pronuclear-stage embryos. Mol Reprod Dev 61:173–179
Boyanova M, Tsanev R, Ivanov I (2002) A modified kynurenine bioassay for quantitative determination of human interferon-γ. Anal Biochem 308:178–181
Chen TL, Lin YL, Lee YL, Yang NS, Chan MT (2004) Expression of bioactive human interferon-gamma in transgenic rice cell suspension cultures. Transgenic Res 13:499–510
Daubener W, Wanagat N, Pilz K, Seghrouchni S, Fischer HG, Hadding U (1994) A new simple bioassay for human IFN-γ. J Immunol Methods 168:39–47
Derynck R, Singh A, Goeddel DV (1983) Expression of the human interferon-gamma cDNA in yeast. Nucl Acids Res 25:1819–1837
Devinoy E, Thépot D, Stinnakre MG, Fontaine ML, Grabowski H, Puissant C, Pavirani A, Houdebine LM (1994) High level production of human growth hormone in the milk of transgenic mice: the upstream region of the rabbit whey acidic protein (WAP) gene targets transgene expression to the mammary gland. Transgenic Res 3:79–89
Dobrovolsky VN, Lagutin OV, Vinogradova TV, Frolova IS, Kuznetsov VP, Larionov OA (1993) Human gamma-interferon expression in the mammary gland of transgenic mice. FEBS Lett 15:181–184
Ebert KM, DiTullio P, Barry CA, Schindler JE, Ayres SL, Smith TE, Pellerin LJ, Meade HM, Denman J, Roberts B (1994) Induction of human tissue plasminogen activator in the mammary gland of transgenic goats. Biotechnology (NY) 12:699–702
Hogan B, Beddigton R, Costantini F, Lacy E (1994) Manipulating the mouse embryo: a laboratory manual, 2nd edn. Cold Spring Harbor Laboratory Press, Cold Spring Harbor
Houdebine LM (1995) The production of pharmaceutical proteins from the milk of transgenic animals. Reprod Nutr Dev 35:609–617
Lagutin OV, Dobrovolsky VN, Vinogradova TV, Kyndiakov BN, Khodarovich YM, Jenkins N, James D, Markham N, Larionov OA (1999) Efficient human IFN-gamma expression in the mammary gland of transgenic mice. J Interf Cytokine Res 19:137–144
Maga EA, Murray JD (1995) Mammary gland expression of transgenes and the potential for altering the properties of milk. Biotechnology 13:1452–1457
Mory Y, Ben-Barak J, Segev D, Cohen B, Novick D, Fischer DG, Rubinstein M, Kargman S, Zilberstein A, Vigneron M (1986) Efficient constitutive production of human IFN-gamma in Chinese hamster ovary cells. DNA 5(3):181–193
Rinderknecht E, O’Connor BH, Rodriguez H (1984) Natural human interferon-gamma: complete amino acid sequence and determination of sites of glycosylation. J Biol Chem 259(11):6790–6797
Shi G, Chen H, Wu X, Zhou Y, Liu Z, Zheng T, Huang T (2009) A mWAP–hLF hybrid gene locus gave extremely high level expression of human lactoferrin in the milk of transgenic mice. Transgenic Res 18:573–582
Smith D, Mayhew ER, Reszka M, Ito M, Omalley J (1990) Antiviral and antiproliferative properties of liposome-associated human interferon-gamma. J Interf Res 10:153–160
Tsanev R, Ivanov I (2001) Immune interferon: properties and clinical applications. CRC Press, Boca Raton
Velander WH, Johnson JL, Page RL, Russell CG, Subramanian A, Wilkins TD, Gwazdauskas FC, Pittius C, Drohan W (1992) High-level expression of a heterologous protein in the milk of transgenic swine using the cDNA encoding human protein C. Proc Natl Acad Sci USA 89:12003–12007
Acknowledgments
This study was conducted in the Tubitak, MRC, GEBI. The authors are grateful to Dr. Batu Erman for providing pTZ57R vector containing hIFN-γ. We thank Sakir Sekmen, Gazi Turgut, Arzu Tas, and Erman Ates for excellent technical assistance. This study was funded by Tubitak (SBAG-BULGAR-1-105S001) and Grant No. IDEI-02/30/17.12.09 from Bulgarian National Research for I. Ivanov.
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Bagis, H., Aktoprakligil, D., Gunes, C. et al. Expression of Biologically Active Human Interferon Gamma in the Milk of Transgenic Mice Under the Control of the Murine Whey Acidic Protein Gene Promoter. Biochem Genet 49, 251–257 (2011). https://doi.org/10.1007/s10528-010-9403-7
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DOI: https://doi.org/10.1007/s10528-010-9403-7