Allozyme analysis, microsatellite primer PCR (SSRP-PCR), and amplified fragment length polymorphism (AFLP) techniques were used to assess genetic diversity and population structure of the Chinese oriental migratory locust, Locusta migratoria manilensis. A total of 299 PCR markers (67 SSRPs and 232 AFLPs) were detected in eight populations, of which 98.7% were polymorphic markers. The proportion of polymorphic loci (95.5–98.8%) by SSRP+AFLP markers indicated no significant differences between populations, and all populations exhibited a similar level of variability; results of the allozyme analysis demonstrated that 19 loci gave rise to a lower level of polymorphism (55.6–66.7%). The genetic distances between the populations were relatively low. Shannon’s index and Nei’s gene diversity showed low differentiation among the populations. Allozyme analysis, however, reflected greater similarity and smaller differentiation between the populations than those shown by SSRP and AFLP markers. Neighbor-joining dendrograms derived from both the allozyme and SSRP+AFLP markers showed that the genetic distances among Chinese oriental migratory locust populations were not greatly influenced by geographic distance and breeding habitats.
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ACKNOWLEDGMENTS
The authors wish to thank Dr. Zhu-Mei Ren for her generous help in the literature review and in theoretical guidance. We thank Dr Peng Nan of Ministry of Education Key Laboratory for Biodiversity Science and Ecological Engineering at Fudan University for help in data analysis. This work was supported by the National Natural Science Foundation of China (30170612 and 30470219) and the Project of the Science and Technology Commission of Shanxi Province (041005).
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Zheng, X., Zhong, Y., Duan, Y. et al. Genetic Variation and Population Structure of Oriental Migratory Locust, Locusta migratoria manilensis, in China by Allozyme, SSRP-PCR, and AFLP Markers. Biochem Genet 44, 332–346 (2006). https://doi.org/10.1007/s10528-006-9033-2
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DOI: https://doi.org/10.1007/s10528-006-9033-2