Abstract
Chronic administration of d-galactose (d-gal) is a useful method for establishing a model of natural aging. However, whether animal age or jet lag influences the d-gal-induced aging process has not been investigated. To clarify these issues, ICR male mice were given a daily injection of d-gal (500 mg/kg bw) for 7 wks. d-gal-treatment caused a significant decrease only in the transcript level of Cat gene in the 6-wk-old mice, whereas it significantly reduced the expression of Cat and Sod1 genes and of Cat, Gpx1, Sod1 and Sod2 genes in the 12- and 22-wk-old group, respectively, suggesting that d-gal induced aging process was greatly dependent on the age of mice. Mice receiving both d-gal and jet lag exhibited larger decreases in the expression levels of above four genes in liver as compared with that of either group singly, indicating a synergistic effect of jet lag and d-gal on the aging process.
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Abbreviations
- d-gal:
-
d-galactose
- RT-PCR:
-
Reverse transcription-polymerase chain reaction
- T-AOC:
-
Total antioxidant capabilities
- ROS:
-
Reactive oxygen species
- Galk :
-
Galactokinase gene
- Galt :
-
Galactose-1-phosphate uridyltransferase gene
References
Cadenas E, Davies KJ (2000) Mitochondrial free radical generation, oxidative stress, and aging. Free Radic Biol Med 29:222–230. doi:10.1016/S0891-5849(00)00317-8
Chen CF, Lang SY, Zuo PP et al (2006) Effects of d-galactose on the expression of hippocampal peripheral-type benzodiazepine receptor and spatial memory performances in rats. Psychoneuroendocrinology 31:805–811. doi:10.1016/j.psyneuen.2006.03.004
Cuatrecasas P, Segal S (1966) Galactose conversion to d-xylulose: an alternate route of galactose metabolism. Science 153:549–551. doi:10.1126/science.153.3735.549
Cui X, Wang LN, Zuo PP et al (2004) d-Galactose-caused life shortening in Drosophila melanogaster and Musca domestica is associated with oxidative stress. Biogerontology 5:317–326. doi:10.1007/s10522-004-2570-3
Cui X, Zuo P, Zhang Q et al (2006) Chronic systemic d-galactose exposure induces memory loss, neurodegeneration, and oxidative damage in mice: protective effects of R-α-lipoic acid. J Neurosci Res 83:1584–1590. doi:10.1002/jnr.20845
Cutler RG, Mattson MP (2006) The adversities of aging. Ageing Res Rev 5:221–238. doi:10.1016/j.arr.2006.05.002
Davidson AJ, Sellix MT, Daniel J et al (2006) Chronic jet-lag increases mortality in aged mice. Curr Biol 16:R914–R916. doi:10.1016/j.cub.2006.09.058
Davies KJ (2000) Oxidative stress, antioxidant defenses, and damage removal, repair, and replacement systems. IUBMB Life 50:279–289. doi:10.1080/15216540051081010
Deng HB, Cui DP, Jiang JM et al (2003) Inhibiting effects of Achyranthes bidentata polysaccharide and Lycium barbarum polysaccharide on nonenzyme glycation in d-galactose induced mouse aging model. Biomed Environ Sci 16:267–275
Gibson JB (1995) Gonadal function in galactosemics and in galactose-intoxicated animals. Eur J Pediatr 154:S14–S20. doi:10.1007/BF02143797
Gitzelmann R (1995) Galactose-1-phosphate in the pathophysiology of galactosemia. Eur J Pediatr 154:S45–S49. doi:10.1007/BF02143803
Ho SC, Liu JH, Wu RY (2003) Establishment of the mimetic aging effect in mice caused by d-galactose. Biogerontology 4:15–18. doi:10.1023/A:1022417102206
Holton JB, Walter JH, Tyfield LA (2001) Galactosemia. In: The metabolic and molecular bases of inherited diseases. McGraw Hill, New York
Hua XD, Lei M, Zhang YJ et al (2007) Long-term d-galactose injection combined with ovariectomy serves as a new rodent model for Alzheimer’s disease. Life Sci 80:1897–1905. doi:10.1016/j.lfs.2007.02.030
Kireev RA, Tresguerres AC, Castillo C et al (2007) Effect of exogenous administration of melatonin and growth hormone on pro-antioxidant functions of the liver in aging male rats. J Pineal Res 42:64–70. doi:10.1111/j.1600-079X.2006.00385.x
Kondratova AA, Gorbacheva VY, Vykhovanets OV et al (2006) Early aging and age-related pathologies in mice deficient in BMAL1, the core component of the circadian clock. Genes Dev 20:1868–1873. doi:10.1101/gad.1432206
Kong WJ, Wang Y, Wang Q et al (2006a) The effect of the mtDNA4834 deletion on hearing. Biochem Biophys Res Commun 344:425–430. doi:10.1016/j.bbrc.2006.03.060
Kong WJ, Wang Y, Wang Q et al (2006b) The relation between d-galactose injection and mitochondrial DNA 4834 bp deletion mutation. Exp Gerontol 41:628–634. doi:10.1016/j.exger.2006.04.008
Kuro-o M, Matsumura Y, Aizawa H et al (1997) Mutation of the mouse klotho gene leads to a syndrome resembling aging. Nature 390:45–51. doi:10.1038/36285
Livak KJ, Schmittgen TD (2001) Analysis of relative gene expression data using real-time quantitative PCR and the 2 -ΔΔCt method. Methods 25:402–408. doi:10.1006/meth.2001.1262
Lu J, Zheng YL, Luo L et al (2006) Quercetin reverses d-galactose induced neurotoxicity in mouse brain. Behav Brain Res 171:251–260. doi:10.1016/j.bbr.2006.03.043
Lu J, Zheng YL, Wu DM et al (2007) Ursolic acid ameliorates cognition deficits and attenuates oxidative damage in the brain of senescent mice induced by d-galactose. Biochem Pharmacol 74:1078–1090. doi:10.1016/j.bcp. 2007.07.007
Melchiorri D, Reiter RJ, Sewerynek E et al (1996) Paraquat toxicity and oxidative damage reduction by melatonin. Biochem Pharmacol 51:1095–1099. doi:10.1016/0006-2952(96)00055-X
Petry K, Greinix HT, Nudelman E et al (1991) Characterization of a novel biochemical abnormality in galactosemia: deficiency of glycolipids containing galactose or N-acetylgalactosamine and accumulation of precursors in brain and lymphocytes. Biochem Med Metab Biol 46:93–104. doi:10.1016/0885-4505(91)90054-O
Pierpaoli W, Maestroni GJ (1987) Melatonin: a principal neuroimmunoregulatory and anti-stress hormone: its anti-aging effects. Immunol Lett 16:355–361. doi:10.1016/0165-2478(87)90169-6
Quan-Ma R, Wells HJ, Wells WW et al (1966) Galactitol in the tissues of a galactosemic child. Am J Dis Child 112:477–478
Rodriguez C, Mayo JC, Sainz RM et al (2004) Regulation of antioxidant enzymes: a significant role for melatonin. J Pineal Res 36:1–9. doi:10.1046/j.1600-079X.2003.00092.x
Salhab M, Mokbel K (2006) Breast cancer risk in flight attendants: an update. Int J Fertil Womens Med 51:205–207
Schadewaldt P, Kamalanathan L, Hammen HW et al (2003) Stable-isotope dilution analysis of galactose metabolites in human erythrocytes. Rapid Commun Mass Spectrom 17:2833–2838. doi:10.1002/rcm.1272
Shen YX, Xu SY, Wei W et al (2002) Melatonin reduces memory changes and neural oxidative damage in mice treated with d-galactose. J Pineal Res 32:173–178. doi:10.1034/j.1600-079x.2002.1o850.x
Song X, Bao M, Li D et al (1999) Advanced glycation in d-galactose induced mouse aging model. Mech Ageing Dev 108:239–251. doi:10.1016/S0047-6374(99)00022-6
Sun SW, Yu HQ, Zhang H et al (2007) Quercetin attenuates spontaneous behavior and spatial memory impairment in d-galactose-treated mice by increasing brain antioxidant capacity. Nutr Res 27:169–175. doi:10.1016/j.nutres.2007.01.010
Van HR (1959) Formation of polyols by the lens of the rat with “sugar” cataracts. Nature 184:194–195
Wang ZI, Berry GT, Dreha SF et al (2001) Proton magnetic resonance spectroscopy of brain metabolites in galactosemia. Ann Neurol 50:266–269. doi:10.1002/ana.1102
Wang H, Gao XD, Zhou GC et al (2008) In vitro and in vivo antioxidant activity of aqueous extract from Choerospondias axillaris fruit. Food Chem 106:888–895. doi:10.1016/j.foodchem.2007.05.068
Wei YH, Lee HC (2002) Oxidative stress, mitochondrial DNA mutation, and impairment of antioxidant enzymes in aging. Exp Biol Med 227:671–682
Wei HF, Li L, Song QJ et al (2005) Behavioural study of the d-galactose induced aging model in C57BL/6J mice. Behav Brain Res 157:245–251. doi:10.1016/j.bbr.2004.07.003
Xu FB (1985) Sub-acute toxicity of d-galactose. In: Proceedings of the second national conference on aging research, Harbin, China
Yager CT, Chen J, Reynolds R et al (2003) Galactitol and galactonate in red blood cells of galactosemic patients. Mol Genet Metab 80:283–289. doi:10.1016/j.ymgme.2003.08.021
Zhang D, Liu GT, Shi JG et al (2006) Coeloglossum viride var. bracteatum extract attenuates d-galactose and NaNO2 induced memory impairment in mice. J Ethnopharmacol 104:250–256. doi:10.1016/j.jep.2005.09.010
Zhang HX, Yu NC, Huang GF et al (2007a) Neuroprotective effects of purslane herb aquenous extracts against d-galactose induced neurotoxicity. Chem Biol Interact 170:145–152. doi:10.1016/j.cbi.2007.07.009
Zhang XL, Jiang B, Li ZB et al (2007b) Catalpol ameliorates cognition deficits and attenuates oxidative damage in the brain of senescent mice induced by d-galactose. Pharmacol Biochem Behav 88:64–72. doi:10.1016/j.pbb.2007.07.004
Acknowledgments
This study was supported by the Program for Changjiang Scholars and Innovative Research Team in University (No. IRT 0653), Science Foundation of Zhejiang Province, China (No. 2004C14003), and Natural Science Foundation of Zhejiang Province for Distinguished Young Students (No. 2007R40G2020043).
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Xu, Y., Wu, T., Jin, Y. et al. Effects of age and jet lag on d-galactose induced aging process. Biogerontology 10, 153–161 (2009). https://doi.org/10.1007/s10522-008-9158-2
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DOI: https://doi.org/10.1007/s10522-008-9158-2