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Antiplatelet Action of Chloramine Derivatives of Adenosine Phosphates and Their Chemical Activity in Relation to Sulfur-Containing Compounds

  • PHARMACOLOGY AND TOXICOLOGY
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Bulletin of Experimental Biology and Medicine Aims and scope

We studied the properties of N6-chloroadenosine phosphates (ATP, ADP, and AMP chloramines) as compounds with potentially increased antiplatelet efficacy determined by their binding to the plasma membrane of platelets. Chloramine derivatives of ATP, ADP, and AMP do not differ in their optical absorption characteristics: their absorption spectra are in the range of 220-340 nm with a maximum at 264 nm. Chloramines of adenosine phosphates are characterized by high reactivity with respect to thiol compounds. In particular, the rate constants of the reaction of N6-chloroadenosine-5’-diphosphate with N-acetylcysteine, reduced glutathione, dithiothreitol, and cysteine reach 59,000, 250,000, 340,000, and 1,250,000 M—1×sec—1, respectively, and only 1.10±0.02 M—1×sec—1 with methionine. It has been found that N6-chloradenosine-5’-triphosphate is a strong inhibitor of platelet functions: it effectively suppresses ADP-induced cell aggregation (IC50 in the whole blood is 5 μM) and inhibits aggregation of preactivated platelets and induces dissociation of their aggregates.

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Authors and Affiliations

  1. Yu. M. Lopukhin Federal Research and Clinical Center of Physical-Chemical Medicine, Federal Medical-Biological Agency of Russia, Moscow, Russia

    M. A. Murina, D. I. Roshchupkin & V. I. Sergienko

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  1. M. A. Murina
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  2. D. I. Roshchupkin
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  3. V. I. Sergienko
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Correspondence to M. A. Murina.

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Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 175, No. 2, pp. 161-165, February, 2023

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Murina, M.A., Roshchupkin, D.I. & Sergienko, V.I. Antiplatelet Action of Chloramine Derivatives of Adenosine Phosphates and Their Chemical Activity in Relation to Sulfur-Containing Compounds. Bull Exp Biol Med 175, 201–204 (2023). https://doi.org/10.1007/s10517-023-05834-x

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  • DOI: https://doi.org/10.1007/s10517-023-05834-x

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