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Modified Estrogen-Progesterone Induction Method of Mammary Gland Hyperplasia in Rats

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In female Wistar rats, mammary gland hyperplasia (MGH) was modeled according to a modified protocol involving estrogen-progesterone induction and taking into account the duration of the estrous cycle of this animal species. MGH was induced over four 7-day cycles; each cycle included subcutaneous administration of 17β-estradiol (0.5 mg/kg) for 4 days, injection of progesterone (5 mg/kg) on day 5, then 2 days without injections. In females with MGH, a significant increase in the height and diameter of the nipples of the mammary glands was recorded, two types of changes were observed in the gland tissue: tubuloalveolar and lobuloalveolar hyperplasia. The study confirmed the development of MGH in rats by a modified method.

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Authors and Affiliations

  1. N. N. Petrov National Medical Research Center of Oncology, Ministry of Health of the Russian Federation, St. Petersburg, Russia

    A. L. Semenov, M. L. Tyndyk, Yu.D. Von, E. A. Radetskaya, A. S. Kruglov, A. A. Dorofeeva, I. V. Mizgirev, E. D. Ermakova & A. V. Panchenko

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  1. A. L. Semenov
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  2. M. L. Tyndyk
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  3. Yu.D. Von
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  4. E. A. Radetskaya
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  5. A. S. Kruglov
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  6. A. A. Dorofeeva
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  7. I. V. Mizgirev
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  8. E. D. Ermakova
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  9. A. V. Panchenko
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Correspondence to A. L. Semenov.

Additional information

Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 175, No. 1, pp. 128-132, January, 2023

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Semenov, A.L., Tyndyk, M.L., Von, Y. et al. Modified Estrogen-Progesterone Induction Method of Mammary Gland Hyperplasia in Rats. Bull Exp Biol Med 175, 112–115 (2023). https://doi.org/10.1007/s10517-023-05821-2

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  • DOI: https://doi.org/10.1007/s10517-023-05821-2

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