Skip to main content
SpringerLink
Log in

Complex Analysis of Total and Fetal DNA and Cytokines in Blood Plasma of Pregnant Women with Preeclampsia

  • Published:
Bulletin of Experimental Biology and Medicine Aims and scope

We performed a complex analysis of total and fetal extracellular DNA, 8 cytokines (IL-2, IL-4, IL-6, IL-8, IL-10, granulocyte-macrophage CSF, IFNγ, and TNFα) in blood plasma obtained from women with preeclampsia prior to labor onset. Total (sensitivity 89.47%, specificity 93.75%) and fetal extracellular DNA (sensitivity 73.68%, specificity 87.5%) were the most accurate parameters determining preeclampsia. We revealed a high correlation (p=3×10—6) between total and fetal extracellular DNA levels in the group of preeclampsia. Preeclampsia significantly increased the levels of macrophage factors IL-10 and IL-6. These cytokines significantly correlated with the levels of total and fetal extracellular DNA in the preeclampsia group. In the control group, such correlations were not observed. These findings obtained suggest that preeclampsia develops upon increased macrophage activity, leading to destruction of the placenta trophoblast cells.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Gracheva MI, Kan NE, Krasniy AM. Role of cell-free fetal DNA in the early diagnosis of pregnancy complications. Akush. Ginekol. 2016;(10):5-11. Russian.

  2. Krasnyi AM, Kan NE, Tyutyunnik VL, Khovkhaeva PA, Volgina NE, Sergunina OA, Tyutyunnik NV, Bednyagin LA. Oxidative stress in preeclampsia and in normal pregnancy. Akush. Ginekol. 2016;(5):90-95. Russian.

  3. Bzowska M, Guzik K, Barczyk K, Ernst M, Flad HD, Pryjma J. Increased IL-10 production during spontaneous apoptosis of monocytes. Eur. J. Immunol. 2002;32(7):2011-2020.

    Article  CAS  PubMed  Google Scholar 

  4. Chan KC, Ding C, Gerovassili A, Yeung SW, Chiu RW, Leung TN, Lau TK, Chim SS, Chung GT, Nicolaides KH, Lo YM. Hypermethylated RASSF1A in maternal plasma: A universal fetal DNA marker that improves the reliability of noninvasive prenatal diagnosis. Clin. Chem. 2006;52(12):2211-2218.

    Article  CAS  PubMed  Google Scholar 

  5. Chen Q, Sousa JD, Snowise S, Chamley L, Stone P. Reduction in the severity of early onset severe preeclampsia during gestation may be associated with changes in endothelial cell activation: A pathological case report. Hypertens. Pregnancy. 2016;35(1):32-41.

    Article  CAS  PubMed  Google Scholar 

  6. Choi JJ, Reich CF 3rd, Pisetsky DS. The role of macrophages in the in vitro generation of extracellular DNA from apoptotic and necrotic cells. Immunology. 2005;115(1):55-62.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  7. Gleicher N, Kushnir VA, Barad DH. Redirecting reproductive immunology research toward pregnancy as a period of temporary immune tolerance. J. Assist. Reprod. Genet. 2017;34(4):425-430.

    Article  PubMed  PubMed Central  Google Scholar 

  8. Pilette C, Detry B, Guisset A, Gabriels J, Sibille Y. Induction of interleukin-10 expression through Fcalpha receptor in human monocytes and monocyte-derived dendritic cells: role of p38 MAPKinase. Immunol. Cell Biol. 2010;88(4):486-493.

    Article  CAS  PubMed  Google Scholar 

  9. Powe CE, Levine RJ, Karumanchi SA. Preeclampsia, a disease of the maternal endothelium: the role of antiangiogenic factors and implications for later cardiovascular disease. Circulation. 2011;123(24):2856-2869.

    Article  PubMed  Google Scholar 

  10. Redman CW, Sargent IL. Pre-eclampsia, the placenta and the maternal systemic inflammatory response--a review. Placenta. 2003;24(Suppl. A):S21-S27.

    Article  PubMed  Google Scholar 

  11. Roberts JM, Taylor RN, Goldfien A. Clinical and biochemical evidence of endothelial cell dysfunction in the pregnancy syndrome preeclampsia. Am. J. Hypertens. 1991;4(8):700-708.

    Article  CAS  PubMed  Google Scholar 

  12. Salvianti F, Inversetti A, Smid M, Valsecchi L, Candiani M, Pazzagli M, Cremonesi L, Ferrari M, Pinzani P, Galbiati S. Prospective evaluation of RASSF1A cell-free DNA as a biomarker of pre-eclampsia. Placenta. 2015;36(9):996-1001.

    Article  CAS  PubMed  Google Scholar 

  13. Szarka A, Rigó J Jr, Lázár L, Beko G, Molvarec A. Circulating cytokines, chemokines and adhesion molecules in normal pregnancy and preeclampsia determined by multiplex suspension array. BMC Immunol. 2010;11:59. doi: https://doi.org/10.1186/1471-2172-11-59.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  14. Wang Y, Lewis DF, Gu Y, Zhao S, Groome LJ. Elevated maternal soluble Gp130 and IL-6 levels and reduced Gp130 and SOCS-3 expressions in women complicated with preeclampsia. Hypertension. 2011;57(2):336-342

    Article  CAS  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. V. I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russian Federation, Moscow, Russia

    A.M. Krasnyi, M. I. Gracheva, A. A. Sadekova, V. V. Vtorushina, I. S. Balashov, N. E. Kan, P. I. Borovikov, L.V. Krechetova & V. L. Tyutyunnik

  2. N. K. Koltsov Institute of Developmental Biology, Moscow, Russia

    A.M. Krasnyi

Authors
  1. A.M. Krasnyi
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. M. I. Gracheva
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. A. A. Sadekova
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. V. V. Vtorushina
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. I. S. Balashov
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. N. E. Kan
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. P. I. Borovikov
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. L.V. Krechetova
    View author publications

    You can also search for this author in PubMed Google Scholar

  9. V. L. Tyutyunnik
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to A.M. Krasnyi.

Additional information

Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 164, No. 12, pp. 686-691, December, 2017

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Krasnyi, A., Gracheva, M.I., Sadekova, A.A. et al. Complex Analysis of Total and Fetal DNA and Cytokines in Blood Plasma of Pregnant Women with Preeclampsia. Bull Exp Biol Med 164, 721–725 (2018). https://doi.org/10.1007/s10517-018-4066-1

Download citation

  • Received:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10517-018-4066-1

Key Words

Search

Navigation