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Application of Tetrameric Recombinant Human Butyrylcholinesterase as a Biopharmaceutical for Amelioration of Symptoms of Acute Organophosphate Poisoning

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We present a procedure for optimizing the expression of recombinant tetrameric butyrylcholinesterase that enables large-scale production with the yield >30 mg/liter (>90 mg/roller bottle). Intravenous injection of the preparation significantly increased survival and decreased the severity of symptoms of poisoning with paraoxon, an organophosphorus toxin.

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Authors and Affiliations

  1. V. V. Shemyakin and Yu. A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russia

    S. S. Terekhov, O. G. Shamborant, I. V. Smirnov & A. G. Gabibov

  2. Affiliated Branch of V. V. Shemyakin and Yu. A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Pushchino, Moscow Region, Russia

    V. A. Palikov, Y. A. Palikova & I. A. Dyachenko

  3. Kazan (Volga Region) Federal University, Kazan, Republic of Tatarstan, Russia

    P. Masson

Authors
  1. S. S. Terekhov
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  2. V. A. Palikov
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  3. Y. A. Palikova
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  4. I. A. Dyachenko
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  5. O. G. Shamborant
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  6. I. V. Smirnov
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  7. P. Masson
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  8. A. G. Gabibov
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Corresponding author

Correspondence to S. S. Terekhov.

Additional information

Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 163, No. 4, pp. 425-430, April, 2016

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Terekhov, S.S., Palikov, V.A., Palikova, Y.A. et al. Application of Tetrameric Recombinant Human Butyrylcholinesterase as a Biopharmaceutical for Amelioration of Symptoms of Acute Organophosphate Poisoning. Bull Exp Biol Med 163, 430–435 (2017). https://doi.org/10.1007/s10517-017-3821-z

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  • DOI: https://doi.org/10.1007/s10517-017-3821-z

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