It is demonstrated that the output optical signal of MTT test is directly proportional to the number of viable cells in the primary culture of mesenchymal cells (skin fibroblasts and mesenchymal stem cells from the bone marrow, placenta, and umbilical cord). The slope of the best curve in coordinates “cell number – optical signal” reflecting specifi c productivity of MTT-formazan characterizes mean dehydrogenase activity of cells and their physiological activity. It was found that in vitro dehydrogenase activity of primary cultures of mesenchymal cells increased during the first 3–5 passages and then tended to decrease. The variant of MTT method presented here can be used for standardization of cell materials.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
V. V. Burunova, N. E. Manturova, G. O. Smirnova, et al., Russ. Med. Zh., 17, No. 17, 1058–1062 (2009).
V. V. Burunova, Yu. G. Suzdal’tseva, A. V. Voronova, et al., Kletochn. Tekhnol. Biol. Med., No. 2, 97–101 (2008).
R. Murray, D. Granner, P. Mayes, and B. Roduell, Human Biochemistry [Russian translation], Moscow (2004).
Yu. V. Suzdal’tseva, V. V. Burunova, I. V. Vakhrushev, et al., Kletochn. Tekhnol. Biol. Med., No. 1, 3–10 (2007).
M. Yu. Schelkanov, V. F. Eremin, I. B. Sakhuriya, et al., Biokhimiya, 64, No. 4, 431–436 (1999).
M. Yu. Shchelkanov, I. B. Sakhuriya, V. V. Burunova, et al., Immunologiya, No. 1, 37–41 (1999).
E. Pearse, Histochemistry Theoretical and Applied [Russian translation], Moscow (1962).
K. N. Yarygin, Yu. G. Suzdal’tseva, V. V. Burunova, et al, Kletochn. Tekhnol. Biol. Med., No. 1, 53–59 (2006).
T. Mosmann, J. Immunol. Methods, 65, Nos. 1–2, 55–63 (1983).
S. Ahmadian, J. Barar, A. A. Saei, et al., J. Vis. Exp., 26, 1191 (2009).
M. V. Berridge, P. M. Herst, A. S. Tan, Biotechnol. Annu Rev., 11, 127–152 (2005).
T. Hayon, A. Dvilansky, O. Shpilberg, and I. Nathan, Leuk. Lymphoma, 44, No. 11, 1957–1962 (2003).
D. T. Loo and J. R. Rillema, Methods Cell. Biol., 57, 251–264 (1998).
J. M. Sargent, Recent Results Cancer Res., 161, 13–25 (2003).
Author information
Authors and Affiliations
Corresponding author
Additional information
Translated from Kletochnye Tehnologii v Biologii i Medicine, No. 2, pp. 63–67, April, 2010
Rights and permissions
About this article
Cite this article
Burunova, V.V., Gisina, A.M., Kholodenko, I.V. et al. Standardization of Biochemical Profile of Mesenchymal Cell Materials by Probing the Level of Dehydrogenase Activity. Bull Exp Biol Med 149, 497–501 (2010). https://doi.org/10.1007/s10517-010-0978-0
Received:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10517-010-0978-0