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Enhanced ambient UVB light affects growth, body condition and the investment in innate and adaptive immunity in three-spined sticklebacks (Gasterosteus aculeatus)

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Abstract

With ongoing environmental change, ultraviolet-B radiation (UVB) reaching the Earth’s surface has increased over recent decades with consequences for terrestrial and also aquatic ecosystems. Despite evidence for direct physiological and immunological responses of aquatic animals following enhanced UVB exposure, studies investigating indirect impacts of ambient UVB radiation are scarce and mainly used only single doses and/or artificially high amounts of UVB. In the present study, the influence of chronic exposure to elevated UVB levels on growth, body condition and immune function was investigated in three-spined sticklebacks (Gasterosteus aculeatus). Fish were kept outdoors for 68 ± 2 days under two different spectral conditions; one group was exposed to natural solar radiation (UVB-normal), while the other group received additional UVB light for four hours daily (UVB-enhanced). Enhanced UVB radiation was within the range of UVB levels measured at the study site. Fish length and weight were determined at the beginning and end of the experiment to compare growth and body condition between the two treatment groups. At the end of the experiment, the splenosomatic index and the granulocyte-to-lymphocyte ratio were determined as immune parameters. Fish from the UVB-enhanced group showed a reduced growth and body condition as well as a lower splenosomatic index compared to the UVB-normal group. Furthermore, UVB-treated fish had a higher granulocyte-to-lymphocyte ratio representing a relatively higher activation of innate compared to adaptive immunity. Consequently, increased but ecologically relevant levels of ambient UVB negatively affect growth and body condition and have a considerable impact on immunity in three-spined sticklebacks.

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Acknowledgements

We are grateful to Frederik Franke for laboratory assistance and the Bakker research group for discussion.

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Authors and Affiliations

  1. Institute for Evolutionary Biology and Ecology, University of Bonn, An der Immenburg 1, 53121, Bonn, Germany

    Simon Vitt, Anna K. Rahn, Lisa Drolshagen, Theo C. M. Bakker & Ingolf P. Rick

  2. Department of Animal Evolutionary Ecology, Institute for Evolution and Biodiversity, University of Münster, Hüfferstrasse 1, 48149, Münster, Germany

    Jörn P. Scharsack

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  1. Simon Vitt
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  2. Anna K. Rahn
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  4. Theo C. M. Bakker
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Vitt, S., Rahn, A.K., Drolshagen, L. et al. Enhanced ambient UVB light affects growth, body condition and the investment in innate and adaptive immunity in three-spined sticklebacks (Gasterosteus aculeatus). Aquat Ecol 51, 499–509 (2017). https://doi.org/10.1007/s10452-017-9632-5

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