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The effect of ozone treatment on experimental colon anastomosis in rats

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Summary

Background

Colonic anastomosis is a common surgical procedure. Complications after such surgery are still a major problem despite advances in techniques. We aimed to evaluate the effects of pre-operative and postoperative ozone administration on the recovery of colonic anastomoses in rats in this study.

Methods

An end-to-end colon anastomosis was created in the left colon under anesthesia in all experiment group animals. The control group was left alone for 4 days. The other two groups were administered intraperitoneal ozone at a dose of 1 mg/kg once a day. Ozone therapy was started 24 h after surgery to one group (ozone) and 1 h before surgery to the other group (pre-ozone). Samples for biochemical measurements were obtained from the operation site after adhesion scoring was performed and the burst pressure measured.

Results

The burst pressure and OH-proline measurements in the ozone group were found to be significantly higher than in the other groups (p ˂ 0.05). These values were not different between the pre-ozone and control groups (p ˃ 0.05). The MDA values of the ozone group were significantly lower but the antioxidant enzyme levels (except superoxide dismutase) were significantly higher than the other two groups. The TNF-α and IL-6 levels of the ozone group were significantly lower while the IL-10 levels were significantly higher compared with the other two groups.

Conclusion

We therefore feel that ozone treatment after surgery could be important in terms of accelerating colon anastomosis recovery. Ozone administration supports recovery by reducing oxidative stress and proinflammatory cytokines.

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References

  1. Ozler M, Akay C, Oter S, Ay H, Korkmaz A. Similarities and differences of hyperbaric oxygen and medical ozone applications. Free Radic Res. 2011;45:1267–78.

    Article  CAS  PubMed  Google Scholar 

  2. Uysal B, Demirbag S, Poyrazoglu Y, Cayci T, Yesildaglar N, Guven A, Sürer I, Korkmaz A. Medical ozone therapy decreases postoperative uterine adhesion formation in rats. Arch Gynecol Obstet. 2012;286:1201–7.

    Article  CAS  PubMed  Google Scholar 

  3. Ersoz N, Ozler M, Altinel O, Sadir S Ozerhan IH, Uysal B, Topal T, Guven A, Korkmaz A. Melatonin prevents peritoneal adhesions in rats. J Gastroenterol Hepatol. 2009;24:1763–7.

    Article  CAS  PubMed  Google Scholar 

  4. Oztas Y, Uysal B, Kaldirim U, Poyrazoglu Y, Yasar M, Cayci T, Cekli Y, Sadir S, Ozler M, Topal T, Oter S, Korkmaz A. Inhibition of iNOS reduces the therapeutic effects of ozone in acute necrotizing pancreatitis: an in vivo animal study. Scand J Clin Lab Invest. 2011;71:370–7.

    Article  CAS  PubMed  Google Scholar 

  5. Wainstein J, Feldbrin Z, Boaz M, Harman-Boehm I. Efficacy of ozone-oxygen therapy for the treatment of diabetic foot ulcers. Diabetes Technol Ther. 2011;13:1255–60.

    Article  CAS  PubMed  Google Scholar 

  6. Marfella R, Luongo C, Coppola A, Luongo M, Capodanno P, Ruggiero R, Mascolo L, Ambrosino I, Sardu C, Boccardi V, Lettieri B, Paolisso G. Use of a non-specific immunomodulation therapy as a therapeutic vasculogenesis strategy in no-option critical limb ischemia patients. Atherosclerosis. 2010;208:473–9.

  7. Braskén P. Healing of experimental colon anastomosis. Eur J Surg Suppl. 1991;566:1–51.

    PubMed  Google Scholar 

  8. Poyrazoglu Y, Yigit T, Harlak A, Mentes O, Gorgulu S, Uzar AI, Kozak O. Effects of prevention of oxidative and nitro-oxidative stress on experimental rat colon anastomosis using acetylcysteine, Ebselen and 1400 w. Acta Chir Belg. 2011;111:26–31.

    CAS  PubMed  Google Scholar 

  9. Wu Z, Vakalopoulos KA, Kroese LF, Boersema GS, Kleinrensink GJ, Jeekel J, Lange JF. Reducing anastomotic leakage by reinforcement of colorectal anastomosis with cyanoacrylate glue. Eur Surg Res. 2013;50:255–61.

    Article  CAS  PubMed  Google Scholar 

  10. Hamzaoğlu I, Karahasanoğlu T, Aydin S, Sahin DA, Carkman S, Sariyar M, Alemdaroğlu K. The effects of hyperbaric oxygen on normal and ischemic colon anastomoses. Am J Surg. 1998;176:458–61.

    Article  PubMed  Google Scholar 

  11. Ozel Turkcu U, Cakmak GK, Demir EO, Bakkal H, Oner MO, Okyay RD, Bassorgun IC, Ciftcioglu MA. The effect of erythropoietin on anastomotic healing of irradiated rats. J Invest Surg. 2012;25:127–35.

    Article  PubMed  Google Scholar 

  12. Lowry OH, Rosebrough NJ, Farr AL, Randall RJ. Protein measurement with the folin-phenol reagent. J Biol Chem. 1951;193:265–75.

    CAS  PubMed  Google Scholar 

  13. Okhawa H, Ohshi N, Yagi K. Assay or lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal Biochem. 1979;95:351–8.

    Article  Google Scholar 

  14. Sun Y, Oberley LW, Ying L. A simple method for clinical assay of superoxide dismutase. Clin Chem. 1988;34:497–500.

    CAS  PubMed  Google Scholar 

  15. Paglia DE, Valentine WN. Studies on the quantitative and qualitative characterisation of erythrocyte glutathione peroxidase. J Lab Clin Med. 1967;70:158–69.

    CAS  PubMed  Google Scholar 

  16. Reddy GK, Enwemeka CS. A simplified method for the analysis of hydroxyproline in biological tissues. Clin Biochem. 1996;29:225–9.

    Article  CAS  PubMed  Google Scholar 

  17. Schierwagen C, Bylund-Fellenius AC, Lundberg C. Improved method for quantification of tissue PMN accumulation measured by myeloperoxidase activity. J Pharmacol Methods. 1990;23:179–86.

    Article  CAS  PubMed  Google Scholar 

  18. Yauw ST, Hoesseini A, Lomme RM, van Goor H. Serosal abrasion of bowel ends does not enhance anastomotic healing. J Surg Res. 2014 (pii: S0022-4804(14)00805–1.)

  19. Seker D, Ergil J, Ozkan D, Akinci M, Yalcindag A, Ginis Z, Seker G, Arik E. The effects of supplementation with a mixture of arginine, glutamine, and beta-hydroxy beta-methylbutyrate on the healing of colon anastomoses. Acta Chir Belg. 2013;113:444–8.

    CAS  PubMed  Google Scholar 

  20. Klein M, Gögenur I, Rosenberg J. Postoperative use of non-steroidal anti-inflammatory drugs in patients with anastomotic leakage requiring reoperation after colorectal resection: cohort study based on prospective data. BMJ. 2012;26:e6166.

    Article  Google Scholar 

  21. Hendriks T, Mastboom WJ. Healing of experimental intestinal anastomoses. Parameters for repair. Dis Colon Rectum. 1990;33:891–901.

    Article  CAS  PubMed  Google Scholar 

  22. Van der Vijver RJ, van Laarhoven CJ, Lomme RM, Hendriks T. Carprofen for perioperative analgesia causes early anastomotic leakage in the rat ileum. BMC Vet Res. 2012;27;8:247.

    Google Scholar 

  23. Iizuka M, Konno S. Wound healing of intestinal epithelial cells. World J Gastroenterol. 2011;7:2161–71.

    Article  Google Scholar 

  24. Cini C, Wolthuis A, D’Hoore A. Peritoneal fluid cytokines and matrix metalloproteinases as early markers of anastomotic leakage in colorectal anastomosis: a literature review and meta-analysis. Colorectal Dis. 2013;15:1070–7.

    CAS  PubMed  Google Scholar 

  25. Tandara AA, Mustoe TA. Oxygen in wound healing–more than a nutrient. World J Surg. 2004;28:294–300.

    Article  PubMed  Google Scholar 

  26. Hernández F, Menéndez S, Wong R. Decrease of blood cholesterol and stimulation of antioxidative response in cardiopathy patients treated with endovenous ozone therapy. Free Radic Biol Med. 1995;19:115–9.

    Article  PubMed  Google Scholar 

  27. Di Paolo N, Bocci V, Gaggiotti E. Ozone therapy. Int J Artif Organs. 2004;27:168–75.

    PubMed  Google Scholar 

  28. Bocci V. Biological and clinical effects of ozone. Has ozone therapy a future in medicine? Br J Biomed Sci. 1999;56:270–9.

    CAS  PubMed  Google Scholar 

  29. Valacchi G, Bocci V. Studies on the biological effects of ozone: 10. Release of factors from ozonated human platelets. Mediators Inflamm. 1999;8:205–9.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  30. Ozler M, Simsek K, Ozkan C, Akgul EO, Topal T, Oter S, Korkmaz A. Comparison of the effect of topical and systemic melatonin administration on delayed wound healing in rats that underwent pinealectomy. Scand J Clin Lab Invest. 2010;70:447–52.

    Article  CAS  PubMed  Google Scholar 

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Author information

Authors and Affiliations

  1. Department of General Surgery, Gulhane Military Medical Academy, Ankara, Turkey

    N. Ersoz

  2. Department of Physiology, Gulhane Military Medical Academy, Ankara, Turkey

    M. Ozler, T. Topal, B. Uysal & A. Korkmaz

  3. Department of General Surgery, Mevki Military Hospital, Ankara, Turkey

    Y. Poyrazoglu

  4. Department of Undersea and Hyperbaric Medicine, Gülhane Military Medical Academy, Ankara, Turkey

    K. Simsek

  5. Department of Public Health, Gulhane Military Medical Academy, Ankara, Turkey

    E. Gocgeldi

Authors
  1. N. Ersoz
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  2. M. Ozler
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  3. T. Topal
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  4. B. Uysal
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  5. Y. Poyrazoglu
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  6. K. Simsek
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  7. E. Gocgeldi
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  8. A. Korkmaz
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Correspondence to M. Ozler.

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Ersoz, N., Ozler, M., Topal, T. et al. The effect of ozone treatment on experimental colon anastomosis in rats. Eur Surg 48, 122–128 (2016). https://doi.org/10.1007/s10353-015-0372-2

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  • DOI: https://doi.org/10.1007/s10353-015-0372-2

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