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Gestational and lactational exposure to dichlorinated bisphenol A induces early alterations of hepatic lipid composition in mice

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Abstract

Objective

Using non-invasive magnetic resonance (MR) techniques and a histological approach, we assessed the outcomes of perinatal exposure at a low dose of 3,3′-DCBPA (2-chloro-4-[1-(3-chloro-4-hydroxyphenyl)-1-methylethyl]phenol) and/or 3,5-DCBPA (2,6-dichloro-4-[1-(4-hydroxyphenyl)-1-methylethyl]phenol) on mice livers.

Materials and methods

Fertilized female Swiss mice were injected intraperitoneally during gestation and lactation with either vehicle control, 20 μg/kg/day of BPA, 3,5-DCBPA, 3,3′-DCBPA or a mixture (mix-DCBPA). Complementary methods were used to evaluate, in male and female pups, (1) liver structure by texture analysis of images obtained through MR imaging (MRI) and histology, (2) hepatic lipid composition through in vivo 1H MR spectroscopy (1H MRS).

Results

Principal component analysis of texture parameters showed no structural modification of the liver with BPA and DCBPA treatments. Accordingly, no hepatic microvesicular steatosis was observed through hematoxylin–eosin staining. Compared to control, MRS revealed no difference in lipid composition for BPA, 3,5-DCBPA or 3,3′-DCBPA groups. However, MRS detected a significant increase in the mix-DCBPA groups for the saturated component of fatty acids (FA), total unsaturated FA bond index and polyunsaturated FA bond index.

Conclusion

Prior to any structural changes, polyunsaturated fatty acids significantly increased in young male and female mice exposed perinatally at a low dose to a mixture of dichlorinated BPA.

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Acknowledgements

We gratefully acknowledge Laurence Turi for her technical support in animal care and Alba Casas Mora who drew the molecules in Fig. 1. The authors declare that there are no conflicts of interest.

Funding

This work was supported by the Region Centre-Val de Loire (France) (Grant number: no. 201400093609 to SM) and Ligue contre le cancer (France) (to ND).

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Author notes

  1. Dounia El Hamrani and Amandine Chepied contributed equally to this work.

Authors and Affiliations

  1. Centre de Biophysique Moléculaire, CNRS UPR4301, Rue Charles Sadron, CS 80054, 45071, Orléans Cedex 2, France

    Dounia El Hamrani, William Même & Sandra Même

  2. Laboratoire Signalisation et Transports Ioniques Membranaires, University of Poitiers, ERL-CNRS 7368, 1 rue Georges Bonnet, TSA 51106, 86073, Poitiers Cedex 09, France

    Amandine Chepied, Marc Mesnil & Norah Defamie

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  1. Dounia El Hamrani
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Contributions

DE participated in the study design, contributed to data acquisition, analysis, interpretation, and drafted the manuscript. AC was involved in study design, data acquisition, analysis and revising the manuscript. WM supervised the data acquisition, analysis, interpretation and revising the manuscript. MM was involved in interpretation and revising the manuscript. ND was involved in study design, data collection, funding application and revising the manuscript. SM supervised the data acquisition, analysis, interpretation and was involved in funding application and revising the manuscript.

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Correspondence to Dounia El Hamrani.

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The authors declare that they have no conflict of interest.

Ethical statement

All applicable international, national, and/or institutional guidelines for the care and use of animals were followed. All procedures performed in studies involving animals were in accordance with the ethical standards of the French Ministry of Agriculture and Forests (Animal Health and Protection Veterinary Service). The protocol has been approved by the local ethics committee “COMETHEA no. 84” (no. 02.160).

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El Hamrani, D., Chepied, A., Même, W. et al. Gestational and lactational exposure to dichlorinated bisphenol A induces early alterations of hepatic lipid composition in mice. Magn Reson Mater Phy 31, 565–576 (2018). https://doi.org/10.1007/s10334-018-0679-7

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  • DOI: https://doi.org/10.1007/s10334-018-0679-7

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