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The influence of fatty acid supply and aldehyde reductase deletion on cyanobacteria alkane generating pathway in Escherichia coli

  • Metabolic Engineering and Synthetic Biology - Short Comunication
  • Published:
Journal of Industrial Microbiology & Biotechnology

Abstract

Cyanobacteria alkane synthetic pathway has been heterologously constructed in many microbial hosts. It is by far the most studied and reliable alkane generating pathway. Aldehyde deformylating oxygenase (i.e., ADO, key enzyme in this pathway) obtained from different cyanobacteria species showed diverse catalytic abilities. This work indicated that single aldehyde reductase deletions were beneficial to Nostoc punctiforme ADO-depended alkane production in Escherichia coli even better than double deletions. Fatty acid metabolism regulator (FadR) overexpression and low temperature increased C18:1 fatty acid supply, and in turn stimulated C18:1-derived heptadecene production, suggesting that supplying ADO with preferred substrate was important to overall alkane yield improvement. Using combinational methods, 1 g/L alkane was obtained in fed-batch fermentation with heptadecene accounting for nearly 84% of total alkane.

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Acknowledgements

This work was supported by the National Natural Science Foundation of China (31170040) and Chinese Academy of Sciences (KGZD-EW-606).

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Authors and Affiliations

  1. CAS Key Laboratory of Microbial Physiological and Metabolic Engineering, Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100101, China

    Juli Wang, Haiying Yu, Xuejiao Song & Kun Zhu

  2. University of Chinese Academy of Sciences, Beijing, 100049, China

    Juli Wang & Xuejiao Song

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  1. Juli Wang
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  2. Haiying Yu
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Correspondence to Kun Zhu.

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Wang, J., Yu, H., Song, X. et al. The influence of fatty acid supply and aldehyde reductase deletion on cyanobacteria alkane generating pathway in Escherichia coli. J Ind Microbiol Biotechnol 45, 329–334 (2018). https://doi.org/10.1007/s10295-018-2032-6

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  • DOI: https://doi.org/10.1007/s10295-018-2032-6

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