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Pseudoxanthomonas bacteria that drive deposit formation of wood extractives can be flocculated by cationic polyelectrolytes

  • Original Paper
  • Published:
Journal of Industrial Microbiology & Biotechnology

Abstract

Runnability problems caused by suspended bacteria in water using industries, have, in contrast to biofilms, received little attention. We describe here that Pseudoxanthomonas taiwanensis, a wide-spread and abundant bacterium in paper machine water circuits, aggregated dispersions of wood extractives ("pitch") and resin acid, under conditions prevailing in machine water circuits (109 cfu ml−1, pH 8, 45°C). The aggregates were large enough (up to 50 μm) so that they could be expected to clog wires and felts and to reduce dewatering of the fiber web. The Pseudoxanthomonas bacteria were negatively charged over a pH range of 3.2–10. Cationic polyelectrolytes of the types used as retention aids or fixatives to flocculate "anionic trash" in paper machines were effective in flocculating the Pseudoxanthomonas bacteria. The polyelectrolyte most effective for this purpose was of high molecular weight (7–8 × 106 g mol−1) and low charge density (1 meq g−1), whereas polyelectrolytes that effectively zeroed the electrophoretic mobility (i.e., neutralized the negative charge) of the bacterium were less effective in flocculating the bacteria. Based on the results, we concluded that the polyelectrolytes functioning by bridging mechanism, rather than by neutralization of the negative charge, may be useful as tools for reducing harmful deposits resulting from interaction of bacteria with wood extractives in warm water industry.

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Acknowledgments

This work was financially supported by TEKES (Finnish Funding Agency for Technology and Innovation, 783/31/07), Oy Keskuslaboratorio-Centrallaboratorium Ab (VTT Technical Research Centre of Finland), Metso Paper Oy, Kemira Oyj, Tamfelt Oyj Abp (Metso Fabrics Oy), Savcor Forest Oy, Fastpap Oy Ab and Millidyne Oy. Academy of Finland grant (118637) "Photobiomics" for the Centre of Excellence is acknowledged. Marja Kärkkäinen is thanked for the technical assistance. Tuula Suortti, Leena Steininger and Hannele Tukiainen are thanked for many kinds of help. Kati Mäenpää and Viikki Science library are acknowledged for the information services and the Faculty of Agr. and For. Instrument Centre for Technical support.

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Authors and Affiliations

  1. Department of Forest Products Technology, Aalto University School of Chemical Technology, PO Box 16300, 00076, Aalto, Finland

    Taina Leino, Per Stenius & Janne Laine

  2. Department of Food and Environmental Sciences, Division of Microbiology, University of Helsinki, PO Box 56, 00014, Helsinki, Finland

    Mari Raulio & Mirja Salkinoja-Salonen

Authors
  1. Taina Leino
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  2. Mari Raulio
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  3. Per Stenius
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  4. Janne Laine
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  5. Mirja Salkinoja-Salonen
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Correspondence to Taina Leino.

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Leino, T., Raulio, M., Stenius, P. et al. Pseudoxanthomonas bacteria that drive deposit formation of wood extractives can be flocculated by cationic polyelectrolytes. J Ind Microbiol Biotechnol 39, 105–114 (2012). https://doi.org/10.1007/s10295-011-1005-9

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  • DOI: https://doi.org/10.1007/s10295-011-1005-9

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