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Interleukin-1β regulates odontogenic ameloblast-associated protein gene transcription in human gingival epithelial cells

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Abstract

Junction epithelium (JE) is located apical to the bottom of the gingival sulcus and binds enamel to hemidesmosomes to protect the periodontal tissue from bacterial infection. Function of odontogenic ameloblast-associated protein (ODAM) is suggested by its expression sites (JE and maturation stage ameloblasts) to be involved in the adhesion between the JE and enamel, and odontogenesis. To analyze the changes in ODAM gene and protein expressions in inflamed gingiva, Ca9-22 gingival epithelial cells were stimulated with 1 ng/ml interleukin-1β (IL-1β) for 3–24 h, and ODAM mRNA and protein levels were analyzed by real-time PCR and Western blotting. Luciferase (LUC) constructs were made ligating various lengths of human ODAM gene promoters and performed LUC analyses in Ca9-22 cells. Gel shift and chromatin immunoprecipitation (ChIP) assays were performed. IL-1β induced ODAM mRNA and protein levels at 6–24 h. IL-1β increased LUC activities of the ODAM gene promoter constructs from − 85 to − 950. These activities were blocked by protein kinase A, tyrosine kinase, mitogen-activated protein (MAP) kinase kinase and phosphoinositide 3-kinase inhibitors. Gel shift and ChIP assays showed that IL-1β induced CCAAT/enhancer-binding protein (C/EBP) β and Yin Yang1 (YY1) binding to C/EBP1, 2, 3, and YY1 elements. These data indicate that IL-1β stimulates ODAM gene transcription mediated through C/EBP1, C/EBP2, C/EBP3, and YY1 elements in the human ODAM gene promoter.

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Funding

This study was supported in part by the Japan Society for the Promotion of Science KAKENHI Grands, Grant-in-Aid for Scientific Research (C), No. 18K09583 and 21K09922 to HT, 18K09582 and 21K09921 to YN, 17K11994 and 20K09945 to YO, and Grant-in-Aid for Research Activity Start-up and Early-Career Scientists, No. 19K21385 and 21K16976 to MYT. Nihon University Multidisciplinary Research Grant (17-019) for 2017–2018 to YO.

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Authors and Affiliations

  1. Departments of Periodontology, Nihon University School of Dentistry at Matsudo, Chiba, 271-8587, Japan

    Yuto Tsuruya, Arisa Yamaguchi, Mizuho Yamazaki-Takai, Jin Zhenyu, Hideki Takai, Yohei Nakayama & Yorimasa Ogata

  2. Research Institute of Oral Science, Nihon University School of Dentistry at Matsudo, Chiba, 271-8587, Japan

    Hideki Takai, Yohei Nakayama & Yorimasa Ogata

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  1. Yuto Tsuruya
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  2. Arisa Yamaguchi
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  3. Mizuho Yamazaki-Takai
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  4. Jin Zhenyu
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  5. Hideki Takai
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  6. Yohei Nakayama
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  7. Yorimasa Ogata
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Correspondence to Yorimasa Ogata.

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Tsuruya, Y., Yamaguchi, A., Yamazaki-Takai, M. et al. Interleukin-1β regulates odontogenic ameloblast-associated protein gene transcription in human gingival epithelial cells. Odontology 110, 557–568 (2022). https://doi.org/10.1007/s10266-022-00689-6

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  • DOI: https://doi.org/10.1007/s10266-022-00689-6

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