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Salivary 8-hydroxyguanosine levels in smokers and non-smokers with chronic periodontitis

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Abstract

This case-controlled clinical trial was performed on the salivary 8-hydroxyguanosine (8-OHdG) levels in smokers and non-smokers with chronic periodontitis after non-surgical periodontal therapy. Subjects (N = 40) with periodontitis (smokers and non-smokers) and with clinically healthy conditions (smokers and non-smokers) were assigned to this study. At baseline, clinical periodontal parameters (plaque index, gingival index, pocket probing depth and clinical attachment levels) were evaluated. Saliva samples were obtained pre- and post-treatment to quantify the 8-OHdG levels using Elisa technique. Subjects diagnosed with chronic periodontitis with smoking habit (CPs) and non-smokers (CPns) received scaling and root planing. In clinically healthy subjects with smoking habit (CHs) and non-smokers (CHns), only oral hygiene tutoring was performed. All clinical measurements and salivary collection were repeated in a 3-month recall interval. Data were analyzed using Anova, Tukey post hoc test and Mann–Whitney ‘U’ tests (P < 0.05). At baseline, CPs and CPns groups showed significantly higher PI, GI, PD and CAL values than those of CHns and CHs (P < 0.001). Baseline salivary levels of 8-OHdG were significantly higher in CPs group (14.775 pg/mL) (P < 0.001) compared to the other groups. All clinical parameters in chronic periodontitis group improved at the 3rd month recall interval, however, with regards to 8-OHdG values, the CP smoker category still had a higher level compared to CP non-smoker. This study reflects an on-going periodontal destructive status in smokers and salivary 8-OHdG levels could be recognized as an oxidative biomarker for determining periodontal tissue destruction.

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Authors and Affiliations

  1. Department of Periodontology, Manipal College of Dental Sciences, Manipal Academy of Higher Education, Manipal University, Manipal, India

    Jothi Varghese

  2. Department of Biochemistry, Kasturba Medical College, Manipal Academy of Higher Education, Manipal University, Manipal, India

    Vinutha Bhat & Yousef Rezaei Chianeh

  3. Department of Public Health Dentistry, Goa Dental College and Hospital, Bambolim, Goa, India

    V. Kamath

  4. Department of Reconstructive Dentistry and Gerodontology, School of Dental Medicine, University of Bern, Bern, Switzerland

    Nadin Al-Haj Husain

  5. Dental Materials Unit, Center for Dental and Oral Medicine, Clinic for Fixed and Removable Prosthodontics and Dental Materials Science, University of Zürich, Plattenstrasse 11, 8032, Zürich, Switzerland

    Mutlu Özcan

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  1. Jothi Varghese
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  2. Vinutha Bhat
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  3. Yousef Rezaei Chianeh
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  4. V. Kamath
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  5. Nadin Al-Haj Husain
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  6. Mutlu Özcan
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Correspondence to Mutlu Özcan.

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Varghese, J., Bhat, V., Chianeh, Y.R. et al. Salivary 8-hydroxyguanosine levels in smokers and non-smokers with chronic periodontitis. Odontology 108, 569–577 (2020). https://doi.org/10.1007/s10266-020-00496-x

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  • DOI: https://doi.org/10.1007/s10266-020-00496-x

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