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hTERT and TP53 deregulation in intestinal-type gastric carcinogenesis in non-human primates

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Abstract

Despite the high incidence, the molecular events involved in intestinal-type gastric carcinogenesis remains unclear. We previously established an intestinal-type gastric carcinogenesis model in Cebus apella, a New World monkey. In the present study, we evaluated hTERT and TP53 mRNA expression, as well as their protein immunoreactivity, in normal mucosa, non-atrophic gastritis, atrophic gastritis, intestinal metaplasia, and intestinal-type gastric cancer samples of non-human primates treated with N-methyl-nitrosourea. In addition, we evaluated the number of TP53 copies in these samples. Although hTERT immunoreactivity was only detected in gastric cancer, a continuous increase of hTERT mRNA expression was observed from non-atrophic gastritis to gastric tumors. No sample presented p53 immunoreactivity. However, we also observed a continuous decrease of TP53 mRNA expression during the sequential steps of gastric carcinogenesis. Moreover, loss of TP53 copies was observed in intestinal metaplasia and gastric cancer samples. Our study highlights that hTERT and TP53 have a key role in intestinal-type gastric cancer initiation.

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Acknowledgments

This study was supported by Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq; MACS and RRB) and Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP; MFL and DQC) as grants and fellowship awards.

Conflict of interest

All authors declare that they have no conflicts of interest.

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Authors and Affiliations

  1. Disciplina de Genética, Departamento de Morfologia e Genética, Universidade Federal de São Paulo, Rua Botucatu 740, São Paulo, SP, CEP 04023-900, Brazil

    Mariana Ferreira Leal, Danielle Queiroz Calcagno & Marília de Arruda Cardoso Smith

  2. Laboratório de Citogenética Humana, Instituto de Ciências Biológicas, Universidade Federal do Pará, Belém, PA, Brazil

    André Salim Khayat, Tanielly Cristina Raiol Silva & Rommel Rodríguez Burbano

  3. Centro Nacional de Primatas, Ministério da Saúde, Ananindeua, PA, Brazil

    José Augusto Pereira Carneiro Muniz

  4. Unidade de Alta Complexidade em Oncologia, Hospital Universitário João de Barros Barreto, Universidade Federal do Pará, Belém, PA, Brazil

    Paulo Pimentel Assumpção

Authors
  1. Mariana Ferreira Leal
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  2. Danielle Queiroz Calcagno
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  3. André Salim Khayat
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  4. Tanielly Cristina Raiol Silva
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  5. José Augusto Pereira Carneiro Muniz
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  6. Paulo Pimentel Assumpção
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  7. Marília de Arruda Cardoso Smith
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  8. Rommel Rodríguez Burbano
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Corresponding author

Correspondence to Mariana Ferreira Leal.

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Leal, M.F., Calcagno, D.Q., Khayat, A.S. et al. hTERT and TP53 deregulation in intestinal-type gastric carcinogenesis in non-human primates. Clin Exp Med 13, 221–224 (2013). https://doi.org/10.1007/s10238-012-0195-4

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  • DOI: https://doi.org/10.1007/s10238-012-0195-4

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