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Measuring Optokinetic Reflex and Vestibulo-Ocular Reflex in Unilateral Vestibular Organ Damage Model of Zebrafish

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Abstract

One-sided vestibular disorders are common in clinical practice; however, their models have not been fully established. We investigated the effect of unilateral or bilateral deficits in the vestibular organs on the vestibulo-ocular reflex (VOR) and optokinetic reflex (OKR) of zebrafish using in-house equipment. For physical dislodgement of the otoliths in the utricles of zebrafish larvae, one or both utricles were separated from the surrounding tissue using glass capillaries. The video data from VOR and OKR tests with the larvae was collected and processed using digital signal processing techniques such as fast Fourier transform and low-pass filters. The results showed that unilateral and bilateral damage to the vestibular system significantly reduced VOR and OKR. In contrast, no significant difference was observed between unilateral and bilateral damage. This study confirmed that VOR and OKR were significantly reduced in zebrafish with unilateral and bilateral vestibular damage. Follow-up studies on unilateral vestibular disorders can be conducted using this tool.

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Availability of Data and Materials

The datasets generated and/or analyzed during the current study are available from the corresponding author upon reasonable request.

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Funding

This research was supported by Ansan-Si hidden champion fostering and supporting project funded by Ansan city, a Korea University Grant, and Basic Science Research Program through the National Research Foundation of Korea (NRF; 2022R1I1A2072826).

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  1. Kang Hyeon Lim and Hong Ki Kim are contributed equally to this work as the first authors.

Authors and Affiliations

  1. Department of Otorhinolaryngology-Head and Neck Surgery, Korea University College of Medicine, Korea University Ansan Hospital, Ansan, Republic of Korea

    Kang Hyeon Lim, Saemi Park, Eunjung Han, Insik Song, Hee Soo Yoon, Yunkyoung Lee, Yoon Chan Rah & June Choi

  2. Department of Electrical Engineering, Korea University College of Engineering, Korea University, Seoul, Republic of Korea

    Hong Ki Kim, Yong Hun Jang & Sang Hyun Lee

  3. Core Research & Development Center, Korea University Ansan Hospital, Ansan, Republic of Korea

    Jaeyoung Kim

  4. Zebrafish Translational Medical Research Center, Korea University, Ansan, Republic of Korea

    Yunkyoung Lee & June Choi

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  1. Kang Hyeon Lim
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Contributions

Kang Hyeon Lim: conceptualization, methodology, and writing of the original draft. Hong Ki Kim: conceptualization, methodology, and writing of the original draft. Saemi Park: investigation and methodology. Eunjung Han: investigation and methodology. Insik Song: investigation. Hee Soo Yoon: investigation. Jaeyoung Kim: investigation. Yunkyoung Lee: investigation. Yong Hun Jang: investigation. Yoon Chan Rah: investigation. Sang Hyun Lee: supervision, writing, review, and editing. June Choi: supervision, writing, review, and editing.

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Lim, K.H., Kim, H.K., Park, S. et al. Measuring Optokinetic Reflex and Vestibulo-Ocular Reflex in Unilateral Vestibular Organ Damage Model of Zebrafish. JARO 25, 167–177 (2024). https://doi.org/10.1007/s10162-024-00936-3

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