Skip to main content

Advertisement

SpringerLink
Log in

Fluorescent microscopy evaluation of diode laser effect on the penetration depth of turmeric (Curcuma longa) extract cream on skin tissues of Wistar rats

  • Original Article
  • Published:
Lasers in Medical Science Aims and scope Submit manuscript

Abstract

The study investigates the effect of diode laser exposure on curcumin’s skin penetration, using turmeric extraction as a light-sensitive chemical and various laser light sources. It uses an in vivo skin analysis method on Wistar strain mice. The lasers are utilized at wavelengths of 403 nm, 523 nm, 661 nm, and 979 nm. The energy densities of the lasers are 20.566 J/cm2, 20.572 J/cm2, 21.162 J/cm2, and 21.298 J/cm2, which are comparable to one another. The experimental animals were divided into three groups: base cream (BC), turmeric extract cream (TEC), and the combination laser (L), BC, and TEC treatment group. Combination light source (LS) with cream (C) was performed with 8 combinations namely 523 nm ((L1 + BC) and (L1 + TEC)), 661 nm ((L2 + BC) and (L2 + TEC)), 403 nm ((L3 + BC) and (L3 + TEC)), and 979 nm ((L4 + BC) and (L4 + TEC)). The study involved applying four laser types to cream-covered and turmeric extract–coated rat skin, with samples scored for analysis. The study found that both base cream and curcumin cream had consistent pH values of 7–8, within the skin’s range, and curcumin extract cream had lower viscosity. The results of the statistical analysis of Kruskal–Wallis showed a significant value (p < 0.05), which means that there are at least two different laser treatments. The results of the post hoc analysis with Mann–Whitney showed that there was no significant difference in the LS treatment with the addition of BC or TEC when compared to the BC or TEC treatment alone (p > 0.05), while the treatment using BC and TEC showed a significant difference (p < 0.05). Laser treatment affects the penetration of the turmeric extract cream into the rat skin tissue.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6
Fig. 7
Fig. 8

Similar content being viewed by others

References

  1. Bunker C, Watchorn R (2023) Skin disease. In Medicine for Finals and Beyond. CRC Press, pp 611–656

    Google Scholar 

  2. Lim JS, Park HS, Cho S, Yoon HS (2018) Antibiotic susceptibility and treatment response in bacterial skin infection. Ann Dermatol 30:186

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  3. Russo A, Concia E, Cristini F, De Rosa FG, Esposito S, Menichetti F, Bassetti M (2016) Current and future trends in antibiotic therapy of acute bacterial skin and skin-structure infections. Clin Microbiol Infect 22:S27–S36

    Article  CAS  PubMed  Google Scholar 

  4. Astuti SD, Arifianto D, Drantantiyas ND, Nasution AM (2016) Efficacy of CNC-diode laser combine with chlorophylls to eliminate Staphylococcus aureus biofilm. In 2016 International Seminar on Sensors, Instrumentation, Measurement and Metrology (ISSIMM). IEEE Xplore 57–61.

  5. Astuti SD, Drantaniyas ND, Putra APSP, Syarom A, Suhariningsih S (2018) Photodynamic effectiveness of laser diode combined with ozone to reduce Staphylicoccus aureus biofilm with exogenous chlorophyll of Dracaena angustifolia leaves. Biomedical Photonics 7:4–20

    Google Scholar 

  6. Astuti SD, Zaidan AH, Setiawati EM, Suhariningsih S (2016) Chlorophyll mediated photodynamic inactivation of blue laser on Streptococcus mutans. In AIP Conference Proceedings. 1718. AIP Publishing

    Google Scholar 

  7. Astuti SD, Mahmud AF, Putra AP, Setiawatie EM, Arifianto D (2020) Effectiveness of bacterial biofilms photodynamic inactivation mediated by curcumin extract, nanodoxycycline and laser diode. Biomed Photonic T9:4–14

    Google Scholar 

  8. Astuti SD, Kharisma DH, Kholimatussa'Diah S, Zaidan AH (2017) In vitro antifungal efficacy of diode laser-activated silver nanoparticles against Candida albicans, AIP Conference Proceedings. UNAIR

  9. Astuti SD, Rulaningtyas R, Putra AP, Arifianto D (2020) The efficacy of photodynamic inactivation with laser diode on Staphylococcus aureus biofilm with various ages of biofilm. Infect Dis Rep 12:8736

    Article  PubMed  PubMed Central  Google Scholar 

  10. Astuti SD, Pratiwi WI, Tanassatha SA, Alamsyah KA, Susilo Y, Khasanah M (2021) Effect of ozone-induced diode laser of photodynamic inactivation on Pseudomonas aeruginosa. Mal J Med Health Sci 17:27–32

    Google Scholar 

  11. Puspita PS, Putra AP, Zaidan AH, Fahmi MZ, Syahrom A, Suhariningsih, (2019) The antifungal agent of silver nanoparticles activated by diode laser as light source to reduce C. albicans biofilms: an in vitro study. Lasers Med Sci 34:929–937

    Article  PubMed  Google Scholar 

  12. Warrier A, Mazumder N, Prabhu S, Satyamoorthy K, Murali TS (2021) Photodynamic therapy to control microbial biofilms. Photodiagn Photodyn Ther 33:102090

    Article  CAS  Google Scholar 

  13. Qiao Y, Ma F, Liu C, Zhou B, Wei Q, Li W, Zhou M (2018) Near-infrared laser-excited nanoparticles to eradicate multidrug-resistant bacteria and promote wound healing. ACS Appl Mater Interfaces 10:193–206

    Article  CAS  PubMed  Google Scholar 

  14. Fekrazad R, Sarrafzadeh A, Kalhori KA, Khan I, Giubellino APR, A, (2018) Improved wound remodeling correlates with modulated TGF-beta expression in skin diabetic wounds following combined red and infrared photobiomodulation treatments. Photochem Photobiol 94:775–779

    Article  CAS  PubMed  Google Scholar 

  15. Ash C, Dubec M, Donne K, Bashford T (2017) Effect of wavelength and beam width on penetration in light-tissue interaction using computational methods. Lasers Med Sci 32:1909–1918

    Article  PubMed  PubMed Central  Google Scholar 

  16. Astuti SD, Ma’rifah ZA, Fitriyah N, Zaidan AH, Arifianto D, Setiawatie EM (2019) The effectiveness of nano-doxycycline activated by diode laser exposure to reduce S. aureus biofilms: an in vitro Study. Photonic Diagnosis Treatment Infect Inflammatory Dis II 10863:180–191

    Google Scholar 

  17. Mustafa FH, Jaafar MS (2013) Comparison of wavelength-dependent penetration depths of lasers in different types of skin in photodynamic therapy. Indian J Phys 87:203–209

    Article  ADS  CAS  Google Scholar 

  18. Astuti WD, Li K, Sato R, Matsukuma H, Shimizu Y, Gao W (2022) A second harmonic wave angle sensor with a collimated beam of femtosecond laser. Appl Sci 12:5211

    Article  CAS  Google Scholar 

  19. Dias LD, Blanco KC, Mfouo-Tynga IS, Inada NM, Bagnato VS (2020) Curcumin as a photosensitizer: from molecular structure to recent advances in antimicrobial photodynamic therapy. J Photochem Photobiol, C 45:100384

    Article  CAS  Google Scholar 

  20. Halevas E, Arvanitidou M, Mavroidi B, Hatzidimitriou AG, Politopoulos K, Alexandratou E, Sagnou M (2021) A novel curcumin gallium complex as photosensitizer in photodynamic therapy: synthesis, structural and physicochemical characterization, photophysical properties and in vitro studies against breast cancer cells. J Mol Struct 1240:130485

    Article  CAS  Google Scholar 

  21. Hosseini A, Hosseinzadeh H (2018) Antidotal or protective effects of Curcuma longa (turmeric) and its active ingredient, curcumin, against natural and chemical toxicities: a review. Biomed Pharmacother 99:411–421

    Article  CAS  PubMed  Google Scholar 

  22. Popuri AK, Pagala B (2013) Extraction of curcumin from turmeric roots. Int J Innov Res Stud 2:289–299

    Google Scholar 

  23. Im K, Kumar D, Ninan E (2015) Enhanced absorption and pharmacokinetics of fresh turmeric (Curcuma Longa L) derived curcuminoids in comparison with the standard curcumin from dried rhizomes. J Funct Foods 17:55–65. https://doi.org/10.1016/j.jff.2015.04.026

    Article  CAS  Google Scholar 

  24. Yadav DK, Sharma K, Dutta A, Kundu A, Awasthi A, Goon A, Saha S (2017) Purity evaluation of curcuminoids in the turmeric extract obtained by accelerated solvent extraction. J AOAC Int 100:586–591

    Article  CAS  PubMed  Google Scholar 

  25. Sahne F, Mohammadi M, Najafpour GD, Moghadamnia AA (2016) Extraction of bioactive compound curcumin from turmeric (Curcuma longa L.) via different routes: a comparative study. Pakistan J Biotechnol 13:173–180

    Google Scholar 

  26. Thangapazham RL, Sharad S, Maheshwari RK (2013) Skin regenerative potentials of curcumin. BioFactors 39:141–149

    Article  CAS  PubMed  Google Scholar 

  27. Shankar R, Sarangi B, Gupta R, Pathak K (2016) Formulation and characterization of polyherbal cream for skin manifestations. J Asian Assoc Schools Pharm 5:360–366

    Google Scholar 

  28. Saraf S, Jeswani G, Kaur CD, Saraf S (2011) Development of novel herbal cosmetic cream with Curcuma longa extract loaded transfersomes for antiwrinkle effect. Afr J Pharm Pharmacol 5(8):1054–1062

    Google Scholar 

  29. Nagata JY, Hioka N, Kimura E, Batistela VR, Terada RSS, Graciano AX, Hayacibara MF (2012) Antibacterial photodynamic therapy for dental caries: evaluation of the photosensitizers used and light source properties. Photodiagn Photodyn Ther 9:122–131

    Article  CAS  Google Scholar 

  30. Lazzari G, Vinciguerra D, Balasso A, Nicolas V, Goudin N, Garfa-Traore M, Mura S (2019) Light sheet fluorescence microscopy versus confocal microscopy: in quest of a suitable tool to assess drug and nanomedicine penetration into multicellular tumor spheroids. Eur J Pharm Biopharm 142:195–203

    Article  CAS  PubMed  Google Scholar 

  31. Stelzer EH, Strobl F, Chang BJ, Preusser F, Preibisch S, McDole K, Fiolka R (2021) Light sheet fluorescence microscopy. Nat Rev Methods Primers 1:73

    Article  CAS  Google Scholar 

  32. Dawson JB, Barker DJ, Ellis DJ, Cotterill JA, Grassam E, Fisher GW, Feather JW (1980) A theoretical and experimental study of light absorption and scattering by in vivo skin. Phys Med Biol 25:695

    Article  CAS  PubMed  Google Scholar 

  33. Gunjan J, Swarnlata S (2014) Topical delivery of Curcuma longa extract loaded nanosized ethosomes to combat facial wrinkles. J Pharm Drug Del Res 3:2–8

    Google Scholar 

  34. Yaqubi AK, Astuti SD, Zaidan AH, Dezy ZIN (2024) Antibacterial effect of red laser-activated silver nanoparticles synthesized with grape seed extract against Staphylococcus aureus and Escherichia coli. Lasers Med Sci 39:47

    Article  PubMed  Google Scholar 

  35. Astuti SD, Utomo IB, Setiawatie EM, Khasanah M, Purnobasuki H, Arifianto D, Alamsyah KA (2021) Combination effect of laser diode for photodynamic therapy with doxycycline on a wistar rat model of periodontitis. BMC Oral Health 21:1–15

    Article  Google Scholar 

  36. Astuti SD, Sulistyo A, Setiawatie EM, Khasanah M, Purnobasuki H, Arifianto D, Syahrom A (2022) An in-vivo study of photobiomodulation using 403 nm and 649 nm diode lasers for molar tooth extraction wound healing in wistar rats. Odontology 110:1–14.

  37. Al-Busaid MM, Akhtar MS, Alam T, Shehata WA (2020) Development and evaluation of herbal cream containing Curcumin from Curcuma longa. Pharm Pharmacol Int J 8:285–289

    Article  Google Scholar 

Download references

Acknowledgements

This research is supported by Grant from International Research Network of Airlangga University no. 1674/UN3.LPPM/PT.01.03/2023

Author information

Authors and Affiliations

  1. Department of Physics, Faculty of Science and Technology, Airlangga University, Surabaya, 60115, Indonesia

    Suryani Dyah Astuti & Amiliyatul Mawaddah

  2. Department of Pharmacognosy and Phytochemistry, Department of Pharmacy, Airlangga University, Surabaya, 60115, Indonesia

    Idha Kusumawati

  3. Biomedical Engineering, Department of Physics, Faculty of Science and Technology, Airlangga University, Surabaya, 60115, Indonesia

    Amalia Fitriana Mahmud

  4. Physics Engineering, Department of Engineering, Institute of Technology Sepuluh Nopember, Surabaya, 60111, Indonesia

    Aulia Muhammad Taufiq Nasution

  5. Department of Physiology, Faculty of Medicine, Airlangga University, Surabaya, 60132, Indonesia

    Bambang Purwanto

  6. Faculty of Engineering, Dr Soetomo University, Surabaya, 60118, Indonesia

    Yunus Susilo

  7. Faculty of Science and Technology, Airlangga University, Surabaya, 60,115, Indonesia

    Ahmad Khalil Yaqubi

  8. Medical Devices and Technology Centre, Universiti Teknologi Malaysia, Bahru, Malaysia

    Ardiansyah Syahrom

Authors
  1. Suryani Dyah Astuti
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Amiliyatul Mawaddah
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Idha Kusumawati
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Amalia Fitriana Mahmud
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Aulia Muhammad Taufiq Nasution
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Bambang Purwanto
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Yunus Susilo
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Ahmad Khalil Yaqubi
    View author publications

    You can also search for this author in PubMed Google Scholar

  9. Ardiansyah Syahrom
    View author publications

    You can also search for this author in PubMed Google Scholar

Contributions

Suryani Dyah Astuti: conceptualization, methodology, validation, writing review and editing, supervision, funding acquisition.

Amiliyatul Mawaddah: conceptualization, methodology, validation, writing—original draft preparation.

Idha Kusumawati: conceptualization, validation, writing—original draft preparation.

Amalia Fitriana Mahmud: conceptualization, methodology, validation.

Aulia Muhammad Taufiq Nasution: conceptualization, validation, writing—original draft preparation.

Bambang Purwanto: conceptualization, validation, writing—original draft preparation.

Yunus Susilo: conceptualization, methodology, validation.

Ahmad Khalil Yaqubi: conceptualization, validation, writing review and editing.

Ardiansyah Syahrom: conceptualization, methodology, validation.

All authors have read and agreed to the published version of the manuscript.

Corresponding author

Correspondence to Suryani Dyah Astuti.

Ethics declarations

Ethics approval and consent to participate

The study was designed according to the “Declaration of Helsinki” and approved by the Ethics Committee of the Faculty of Dentistry, Airlangga University (ethical clearance No. 736-KE).

Informed consent

Optional.

Competing interests

The authors declare no competing interests.

Additional information

Publisher's Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Rights and permissions

Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Astuti, S.D., Mawaddah, A., Kusumawati, I. et al. Fluorescent microscopy evaluation of diode laser effect on the penetration depth of turmeric (Curcuma longa) extract cream on skin tissues of Wistar rats. Lasers Med Sci 39, 79 (2024). https://doi.org/10.1007/s10103-024-04020-3

Download citation

  • Received:

  • Accepted:

  • Published:

  • DOI: https://doi.org/10.1007/s10103-024-04020-3

Keywords

Search

Navigation