Skip to main content
SpringerLink
Log in

Photobiomodulation therapy with light-emitting diode in stimulating adipose tissue mitochondria

  • Original Article
  • Published:
Lasers in Medical Science Aims and scope Submit manuscript

Abstract

Low-level laser therapy (LLLT) is known for its ability to induce a photochemical process, primarily targeting mitochondria, a process referred to as photobiomodulation (PBM). Recently, its use has been attributed as an adjunct in obesity treatment, to stimulate lipolysis and apoptosis. However, the pathway of stimulation remains uncertain. Thus, the objective of this study was to understand whether mitochondrial stimulation occurs in adipose tissue cells after PBM therapy, which could lead to the processes of lipolysis and apoptosis. A non-randomized clinical trial was conducted using a split abdomen design in obese women who received red and infrared LED photobiomodulation therapy (PBMT). The patients underwent bariatric surgery, and adipose tissue samples were collected for immunohistochemical analysis with primary mitochondrial antibodies. Adipose tissue samples subjected to LED intervention exhibited positivity in mitochondrial antibodies for cAMP, DRP1, FAS, FIS1, MFN2, and OPA1 (p<0.001) compared to the control group. In conclusion, we observed that PBMT was capable of generating mitochondrial stimulation in adipose tissue cells, as evidenced by the positive antibody signals. This finding suggests that mitochondrial stimulation could be the mechanism and action underlying adipose tissue lipolysis and apoptosis.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4

Similar content being viewed by others

References

  1. Mansano BSDM, da Rocha VP, Antonio EL, Peron DF, do Nascimento de Lima R, Tucci PJF, Serra AJ. (2021) Enhancing the therapeutic potential of mesenchymal stem cells with light-emitting diode: implications and molecular mechanisms. Oxidative Med Cell Longev 3:6663539

    Google Scholar 

  2. Huang YY, Sharma SK, Carroll J, Hamblin MR (2011) Biphasic dose response in low level light therapy - an update. Dose-Response 9(4):602–618

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  3. Huang A, Nguyen JK, Jagdeo J (2020) Light-emitting diode-based photodynamic therapy for photoaging, scars, and dyspigmentation: a systematic review. Dermatol Surg 46(11):1388–1394

    Article  CAS  PubMed  Google Scholar 

  4. Jagdeo J, Austin E, Mamalis A, Wong C, Ho D, Siegel DM (2018) Light-emitting diodes in dermatology: a systematic review of randomized controlled trials. Lasers Surg Med 50(6):613–628

    Article  PubMed  PubMed Central  Google Scholar 

  5. Nassab R (2015) The evidence behind noninvasive body contouring devices. Aesthet Surg J 35(3):279–293

    Article  PubMed  Google Scholar 

  6. Campos RMS, Dâmaso AR, Masquio DCL et al (2018) The effects of exercise training associated with low-level laser therapy on biomarkers of adipose tissue transdifferentiation in obese women. Lasers Med Sci 33(6):1245–1254

    Article  Google Scholar 

  7. Liu PF, Hu YC, Kang BH, Tseng YK, Wu PC, Liang CC, Hou YY, Fu TY, Liou HH, Hsieh IC, Ger LP, Shu CW (2017) Expression levels of cleaved caspase-3 and caspase-3 in tumorigenesis and prognosis of oral tongue squamous cell carcinoma. PLoS One 12(7):e0180620

  8. Modena DAO, Soares CD, Martignago CCS, Almeida S, Cazzo E, Chaim EA (2022) Effects of LED photobiomodulation therapy on the subcutaneous fatty tissue of obese individuals - histological and immunohistochemical analysis. s 8:1–7

    Google Scholar 

  9. Xie D, Li YL, Wang GF, Jiang J, Sun LR (2021) Ultraviolet light-emitting diode irradiation induces reactive oxygen species production and mitochondrial membrane potential reduction in HL-60 cells. J Int Med Res 49(5):3000605211016623

  10. Chaim EA, Pareja JC, Gestic MA et al (2017) Programa multidisciplinar pré-operatório de cirurgia bariátrica: uma proposta para o Sistema Único de Saúde. Arq Gastroenterol 54(1):70–74

    Article  PubMed  Google Scholar 

  11. National Institutes of Health (1992) Gastrointestinal surgery for severe obesity: National Institutes of Health Consensus Development Conference statement. Am J Clin Nutr 55(l):615S–619S

    Google Scholar 

  12. Mester E, Mester AE, Mester A (1985) The biomedical effect of laser application. Lasers Surg Med 5(1):31–39

    Article  CAS  PubMed  Google Scholar 

  13. Nicholls DG (2002) Mitochondrial function and dysfunction in the cell: its relevance to aging and aging-related disease. Int J Biochem Cell Biol 34(11):1372–1381

    Article  CAS  PubMed  Google Scholar 

  14. Karu TI (2010) Multiple roles of cytochrome c oxidase in mammalian cells under action of red and IR-A radiation. IUBMB Life 62(8):607–610

    Article  CAS  PubMed  Google Scholar 

  15. de Freitas LF, Hamblin MR (2016) Proposed mechanisms of photobiomodulation or low-level light therapy. IEEE J Sel Top Quantum Electron 22(3):348–364

    Article  Google Scholar 

  16. Wu S, Zhou F, Zhang Z, Xing D (2011a) Mitochondrial oxidative stress causes mitochondrial fragmentation via differential modulation of mitochondrial fission–fusion proteins. FEBS J 278(6):941–954

    Article  CAS  PubMed  Google Scholar 

  17. Gwinn DM, Shackelford DB, Egan DF et al (2008) AMPK phosphorylation of raptor mediates a metabolic checkpoint. Mol Cell 30(2):214–226

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  18. Suen DF, Norris KL, Youle RJ (2008) Mitochondrial dynamics and apoptosis. Genes Dev 22(12):1577–1590

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  19. Chang CR, Blackstone C (2010) Dynamic regulation of mitochondrial fission through modification of the dynamin-related protein Drp1. Ann N Y Acad Sci 1201:34–39

  20. Nomura J, Matsumoto K, Iguchi-Ariga SMM et al (2005) Positive regulation of Fas gene expression by MSSP and abrogation of Fas-mediated apoptosis induction in MSSP-deficient mice. Exp Cell Res 305(2):324–332

    Article  CAS  PubMed  Google Scholar 

  21. Olichon L, Baricault N, Gas E et al (2003) Loss of OPA1 perturbates the mitochondrial inner membrane structure and integrity, leading to cytochrome-c release and apoptosis. J Biol Chem 278:7743–7746

    Article  CAS  PubMed  Google Scholar 

  22. John GB, Shang Y, Li L et al (2005) The mitochondrial inner membrane protein mitofilin controls cristae morphology. Mol Biol Cell 16:1543–1554

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  23. Bach D, Pich S, Soriano FX et al (2003) Mitofusin-2 determines mitochondrial network architecture and mitochondrial metabolism. A novel regulatory mechanism altered in obesity. J Biol Chem 278:17190–17197

    Article  CAS  PubMed  Google Scholar 

  24. Pich S, Bach D, Briones P et al (2005) The Charcot-Marie-Tooth type 2A gene product, Mfn2, up-regulates fuel oxidation through the expression of OXPHOS system. Hum Mol Genet 14:1405–1415

    Article  CAS  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Surgery, Medical Sciences Institute, Campinas University (Unicamp), São Paulo, Brazil

    Débora Aparecida Oliveira Modena, Everton Cazzo & Elinton Adami Chaim

  2. Department of Health Sciences, Postgraduate Program in Rehabilitation and Functional Performance, Ribeirão Preto Medical School, University of São Paulo, Ribeirão Preto, São Paulo, Brazil

    Débora Aparecida Oliveira Modena, Ana Paula Ferro & Elaine Caldeira de Oliveira Guirro

Authors
  1. Débora Aparecida Oliveira Modena
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Ana Paula Ferro
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Elaine Caldeira de Oliveira Guirro
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Everton Cazzo
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Elinton Adami Chaim
    View author publications

    You can also search for this author in PubMed Google Scholar

Contributions

PhD. D. A. O. M., data collection and analysis, writing of the article. MSc. A. P. F., data collection and analysis, writing of the article. PhD. E. C. de O. G., contributions in the writing of the article. PhD. E. C., data collection, joint supervision of the research and contributions in the writing of the article. PhD. E. A. C., data collection, supervision of the research and contributions in the writing of the article.

Corresponding author

Correspondence to Débora Aparecida Oliveira Modena.

Ethics declarations

Ethical approval

The research was not submitted to an ethics committee, as it was a review study.

Conflict of interest

The authors declare no competing interests.

Additional information

Publisher’s Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Rights and permissions

Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Modena, D.A.O., Ferro, A.P., de Oliveira Guirro, E.C. et al. Photobiomodulation therapy with light-emitting diode in stimulating adipose tissue mitochondria. Lasers Med Sci 38, 238 (2023). https://doi.org/10.1007/s10103-023-03906-y

Download citation

  • Received:

  • Accepted:

  • Published:

  • DOI: https://doi.org/10.1007/s10103-023-03906-y

Keywords

Search

Navigation