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Red LED light therapy associated with epidermal growth factor on wound repair process in rats

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Abstract

Epidermal growth factor (EGF) and light-emitting diode (LED) are currently deployed as promissory treatments for skin repair; however, the mechanisms of their association are not yet evidenced. Thus, the present study aimed to evaluate the effects of combined treatment with EGF and red LED on the wound healing processes in rats. Adult Wistar rats were randomized in control group (CG) wounds without treatment; wounds submitted to EGF treatment (EGF); wounds submitted to LED treatment (LED); wounds submitted to EGF associated with LED treatments (EGF/LED). Treatments were performed immediately after the surgical procedure and each 24 h, totaling 8 sessions. Moreover, LED was applied before EGF treatment at a single point in the center of the wound. Morphological characteristics and the immunoexpression of COX-2, VEGF, and TGF-β were measured. The results demonstrated that EGF/LED group presented a higher wound healing index. Additionally, all experimental groups presented similar findings in the histological evaluation, the degree of inflammation, and the area of dermis-like tissue. However, for EGF-treated animals (with or without LED), neoepithelial length was higher. Furthermore, all the treated groups decreased COX-2 and increased VEGF immunoexpression, and only EGF/LED group enhanced the TGF-β protein expression when compared to the untreated group. This research shows that EGF and LED modulate inflammatory process and increase the vascularity. In addition, treatment of EGF associated with LED promoted a more evident positive effect for increasing TGF-β expression and may be promising resources in the clinical treatment of cutaneous wounds.

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CEUA no 3390150419 (ID 008535).

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Authors and Affiliations

  1. Department of Biosciences, Federal University of São Paulo, 136, Silva Jardim Street, Santos, SP, 11015-020, Brazil

    Márcia Busanello-Costa, Ana Claudia Muniz Renno, Cintia Pereira de Goes Santos, Hananiah Tardivo Quintana, Cintia Cristina Santi Martignago & Lívia Assis

  2. Department of Physiotherapy, Federal University of São Carlos, São Carlos, SP, Brazil

    Cintia Cristina Santi Martignago

  3. Scientific Institute and Technological Department-University Brazil, São Paulo-Itaquera, SP, Brazil

    Carla Roberta Tim & Lívia Assis

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  1. Márcia Busanello-Costa
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Correspondence to Lívia Assis.

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This study was approved by the Ethics Committee on the Use of Animals under number 3390154019 and conducted according to the international norms of ethics on animal experimentation (National Research Council, 1996).

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Busanello-Costa, M., Renno, A.C.M., de Goes Santos, C.P. et al. Red LED light therapy associated with epidermal growth factor on wound repair process in rats. Lasers Med Sci 38, 36 (2023). https://doi.org/10.1007/s10103-022-03701-1

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