Abstract
This study was conducted in order to evaluate the role of low-level laser treatment (LLLT) in hair growth in C3H/HeJ mice. Healthy C57BL/6 mice were randomly divided into two groups: with and without low-level laser treatment. The skin color of each mouse was observed each day. Skin samples were collected for H&E, immunofluorescence, PCR, and western blot analysis, to observe the morphology of hair follicles and detect the expression levels of Wnt10b and β-catenin. Observation of skin color demonstrated that black pigmentation started significantly earlier in the laser group than in the control group. Hair follicle number in both groups showed no difference; however, the hair follicle length presented a significant difference. Wnt10b protein was detected on the second day in hair matrix cells in the LLLT group but not in the control group. PCR and western blot results both illustrated that expression of Wnt10b and β-catenin was significantly higher in the LLLT group than in the control group. Our study illustrated that low-level laser treatment can promote hair regrowth by inducing anagen phase of hair follicles via initiating the Wnt10b/β-catenin pathway.
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References
McElwee KJ, Shapiro JS (2012) Promising therapies for treating and/or preventing androgenic alopecia. Skin Therapy Lett 17(6):1–4
Mester E et al (1968) Studies on the inhibiting and activating effects of laser beams. Langenbecks Arch Chir 322:1022–1027
Bjordal JM et al (2003) A systematic review of low level laser therapy with location-specific doses for pain from chronic joint disorders. Aust J Physiother 49(2):107–116
Al Rashoud AS et al (2014) Efficacy of low-level laser therapy applied at acupuncture points in knee osteoarthritis: a randomised double-blind comparative trial. Physiotherapy 100(3):242–248
Gautam AP et al (2012) Low level laser therapy for concurrent chemoradiotherapy induced oral mucositis in head and neck cancer patients—a triple blinded randomized controlled trial. Radiother Oncol 104(3):349–354
Olivieri L et al (2014) Efficacy of low-level laser therapy on hair regrowth in dogs with noninflammatory alopecia: a pilot study. Vet Dermatol 26(1):35–e11
Wikramanayake TC et al (2013) Low-level laser treatment accelerated hair regrowth in a rat model of chemotherapy-induced alopecia (CIA). Lasers Med Sci 28(3):701–706
Jimenez JJ et al (2014) Efficacy and safety of a low-level laser device in the treatment of male and female pattern hair loss: a multicenter, randomized, sham device-controlled, double-blind study. Am J Clin Dermatol 15(2):115–127
Kim H et al (2013) Low-level light therapy for androgenetic alopecia: a 24-week, randomized, double-blind, sham device-controlled multicenter trial. Dermatol Surg 39(8):1177–1183
Chen D et al (2012) Dermal beta-catenin activity in response to epidermal Wnt ligands is required for fibroblast proliferation and hair follicle initiation. Development 139(8):1522–1533
Li YH et al (2011) Wnt10b promotes growth of hair follicles via a canonical Wnt signalling pathway. Clin Exp Dermatol 36(5):534–540
Lien WH et al (2014) In vivo transcriptional governance of hair follicle stem cells by canonical Wnt regulators. Nat Cell Biol 16(2):179–190
Choi YS et al (2013) Distinct functions for Wnt/beta-catenin in hair follicle stem cell proliferation and survival and interfollicular epidermal homeostasis. Cell Stem Cell 13(6):720–733
Ito M et al (2007) Wnt-dependent de novo hair follicle regeneration in adult mouse skin after wounding. Nature 447(7142):316–320
Li YH et al (2013) Adenovirus-mediated Wnt10b overexpression induces hair follicle regeneration. J Invest Dermatol 133(1):42–48
Ouji Y et al (2006) Promotion of hair follicle development and trichogenesis by Wnt-10b in cultured embryonic skin and in reconstituted skin. Biochem Biophys Res Commun 345(2):581–587
Gavish L et al (2004) Low level laser irradiation stimulates mitochondrial membrane potential and disperses subnuclear promyelocytic leukemia protein. Lasers Surg Med 35(5):369–376
Oron U et al (2007) Ga-As (808 nm) laser irradiation enhances ATP production in human neuronal cells in culture. Photomed Laser Surg 25(3):180–182
Yu W et al (1997) Photomodulation of oxidative metabolism and electron chain enzymes in rat liver mitochondria. Photochem Photobiol 66(6):866–871
Olerud JE et al (1988) An assessment of human epidermal repair in elderly normal subjects using immunohistochemical methods. J Invest Dermatol 90(6):845–850
Bickenbach JR, McCutecheon J, Mackenzie IC (1986) Rate of loss of tritiated thymidine label in basal cells in mouse epithelial tissues. Cell Tissue Kinet 19(3):325–333
Muller-Rover S et al (2001) A comprehensive guide for the accurate classification of murine hair follicles in distinct hair cycle stages. J Invest Dermatol 117(1):3–15
Ayer-LeLievre C et al (1988) Expression of the beta-nerve growth factor gene in hippocampal neurons. Science 240(4857):1339–1341
Ayer-LeLievre C et al (1988) Nerve growth factor mRNA and protein in the testis and epididymis of mouse and rat. Proc Natl Acad Sci U S A 85(8):2628–2632
Kwack MH et al (2011) Minoxidil activates beta-catenin pathway in human dermal papilla cells: a possible explanation for its anagen prolongation effect. J Dermatol Sci 62(3):154–159
Chung H et al (2012) The nuts and bolts of low-level laser (light) therapy. Ann Biomed Eng 40(2):516–533
Avram MR, Rogers NE (2009) The use of low-level light for hair growth: part I. J Cosmet Laser Ther 11(2):110–117
Gundogan C, Greve B, Raulin C (2004) Treatment of alopecia areata with the 308-nm xenon chloride excimer laser: case report of two successful treatments with the excimer laser. Lasers Surg Med 34(2):86–90
Shukla S et al (2010) Effect of helium-neon laser irradiation on hair follicle growth cycle of Swiss albino mice. Skin Pharmacol Physiol 23(2):79–85
Moe RH et al (2007) Effectiveness of nonpharmacological and nonsurgical interventions for hip osteoarthritis: an umbrella review of high-quality systematic reviews. Phys Ther 87(12):1716–1727
Su KJ et al (2016) Decoction and fermentation of selected medicinal herbs promote hair regrowth by inducing hair follicle growth in conjunction with Wnts signaling. Evid Based Complement Alternat Med 2016(1):1–10
Ouji Y et al (2008) Wnt-10b, uniquely among Wnts, promotes epithelial differentiation and shaft growth. Biochem Biophys Res Commun 367(2):299–304
Reddy S et al (2001) Characterization of Wnt gene expression in developing and postnatal hair follicles and identification of Wnt5a as a target of Sonic hedgehog in hair follicle morphogenesis. Mech Dev 107(1–2):69–82
Lin WH et al (2015) Fibroblast growth factors stimulate hair growth through beta-catenin and Shh expression in C57BL/6 mice. Biomed Res Int 2015:730139
Doerks T et al (2002) Systematic identification of novel protein domain families associated with nuclear functions. Genome Res 12(1):47–56
Bierie B et al (2003) Activation of beta-catenin in prostate epithelium induces hyperplasias and squamous transdifferentiation. Oncogene 22(25):3875–3887
Whiting DA (2003) Histopathologic features of alopecia areata: a new look. Arch Dermatol 139(12):1555–1559
Ahmed Z et al (2010) Histopathological changes in different stages of alopecia areata. Mymensingh Medical Journal 19(1):100–105
Wikramanayake TC et al (2012) Effects of the Lexington LaserComb on hair regrowth in the C3H/HeJ mouse model of alopecia areata. Lasers Med Sci 27(2):431–436
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All procedures performed in this study involving animals were in accordance with the ethical standards of the Animal Care and Use Committee of the Chinese Academy of Medical Sciences.
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Zhang, T., Liu, L., Fan, J. et al. Low-level laser treatment stimulates hair growth via upregulating Wnt10b and β-catenin expression in C3H/HeJ mice. Lasers Med Sci 32, 1189–1195 (2017). https://doi.org/10.1007/s10103-017-2224-8
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DOI: https://doi.org/10.1007/s10103-017-2224-8