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Detection of Trichomonas vaginalis by Allplex™ STI Essential Assay (Seegene) in clinical samples from the Brazilian public health system users

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Abstract

The failures in Trichomonas vaginalis (TV) infection diagnosis leave more than half of cases unidentified. In this report, urine and vaginal discharge samples were analyzed by wet mount, culture examination, and real-time PCR by Allplex™ (Seegene®) kit, in a population assisted by the Brazilian Public Health System. From 747 samples, 2.81% were positive for TV in wet mount and culture, and 3.88% by Allplex™. Samples kept at − 80 ºC for 22 months did not impair the PCR technique. The sensitivity for wet mount, culture, and Allplex™ was 72, 100, and 100%, respectively. Allplex™ technique showed highest detection of TV.

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Data availability

The datasets generated during and/or analyzed during the current study are not publicly available due to originality but are available from the corresponding author on reasonable request.

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Acknowledgements

The authors acknowledge Seegene for donation of Allplex ™ STI Essential Assay™ (Seegene®) kits and Professor Dr. Gabriela Cybis, Departamento de Estatística, Instituto de Matemática e Estatística, Universidade Federal do Rio Grande do Sul, Porto Alegre, Rio Grande do Sul, Brazil, for the statistical analysis.

Funding

This study received financial support from Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq/Brazil) grant 428538/2018–5 and Fundação de Apoio à Pesquisa do Estado do Rio Grande do Sul (FAPERGS grant 21/2551–0000128-3). M.D.F. received Programa de Pós-Graduação em Ciências Farmacêuticas, Faculdade de Farmácia, UFRGS. T.T. received researcher fellowship (grant 309764/2021–1) from CNPq.

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Authors and Affiliations

  1. Faculdade de Farmácia and Centro de Biotecnologia, Universidade Federal do Rio Grande do Sul, Av. Ipiranga 2752, Porto Alegre, Rio Grande do Sul, 90610-000, Brazil

    Mariana Dicki Freitas, Fernanda Gomes Cardoso, Graziela Vargas Rigo & Tiana Tasca

  2. Laboratório de Biologia Molecular, Microbiologia e Sorologia, Centro de Ciências da Saúde, Universidade Federal de Santa Catarina, Florianópolis, Santa Catarina, Brazil

    Hanalydia de Melo Machado & Maria Luiza Bazzo

Authors
  1. Mariana Dicki Freitas
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  2. Fernanda Gomes Cardoso
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  3. Graziela Vargas Rigo
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  4. Hanalydia de Melo Machado
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  5. Maria Luiza Bazzo
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  6. Tiana Tasca
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Contributions

Tiana Tasca and Mariana Dicki Freitas contributed to the study conception and design. Material preparation and data collection were performed by Mariana Dicki Freitas, Fernanda Gomes Cardoso, Graziela Vargas Rigo, and Hanalydia de Melo Machado. Data analysis was conducted by Mariana Dicki Freitas, Maria Luiza Bazzo, and Tiana Tasca. The first draft of the manuscript was written by Mariana Dicki Freitas, and all authors commented on previous versions of the manuscript. All authors read and approved the final manuscript.

Corresponding author

Correspondence to Tiana Tasca.

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Ethics approval

This study was performed in line with the principles of the Declaration of Helsinki. This study was approved by the Research Ethics Committee of UFRGS, under assent number 3.198.585.

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Informed consent was obtained from all individual participants included in the study.

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Not applicable since the manuscript contains no individual person’s data in any form.

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The authors declare no competing interests.

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Freitas, M.D., Cardoso, F.G., Rigo, G.V. et al. Detection of Trichomonas vaginalis by Allplex™ STI Essential Assay (Seegene) in clinical samples from the Brazilian public health system users. Eur J Clin Microbiol Infect Dis 43, 167–170 (2024). https://doi.org/10.1007/s10096-023-04694-7

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  • DOI: https://doi.org/10.1007/s10096-023-04694-7

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