Skip to main content

Advertisement

SpringerLink
Log in

Molecular characterization of Mycoplasma pneumoniae infections in Moscow from 2015 to 2018

  • Original Article
  • Published:
European Journal of Clinical Microbiology & Infectious Diseases Aims and scope Submit manuscript

Abstract

The aim of this study was to assess which Mycoplasma pneumoniae genotypes were present in Moscow during the years 2015–2018 and whether the proportion between detected genotypes changed over time. We were also interested in the presence of macrolide resistance (MR)Mycoplasma pneumoniae. We performed multilocus variable-number tandem-repeat (VNTR) analysis (MLVA), SNP typing, and mutation typing in the 23S rRNA gene from 117 M. pneumoniae clinical isolates. Our analysis suggests two major MLVA types: 4572 and 3562. In 2017–2018, MLVA type 4572 gradually became predominant. In general, the SNP type range is the same as described earlier for European countries. The analysis of MR mutations showed that 7% of the isolates had an A2063G mutation in the 23S rRNA gene with no isolates carrying an A2064G mutation. In 2017–2018, MLVA type 4572 (SNP type 1) begins to spread in Moscow, which was widespread globally, especially in Asian countries. SNP typing of our sample showed higher discriminatory power than MLVA typing.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2

Similar content being viewed by others

References

  1. Rachina SA, Kozlov RS, Shal EP, Ustyuzhanin IV, Krechikov OI, Ivanchik NV et al (2011) A spectrum of causative bacterial pathogens in community-acquired pneumonia in multidisciplinary hospitals of Smolensk. Russ Pulmonol 1:5–18. https://doi.org/10.18093/0869-0189-2011-0-1-5-18

  2. Dégrange S, Cazanave C, Charron A, Renaudin H, Bébéar C, Bébéar CM (2009) Development of multiple-locus variable-number tandem-repeat analysis for molecular typing of Mycoplasma pneumoniae. J Clin Microbiol 47:914–923. https://doi.org/10.1128/JCM.01935-08

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  3. Benitez AJ, Diaz MH, Wolff BJ, Pimentel G, Njenga MK, Estevez A et al (2012) Multilocus variable-number tandem-repeat analysis of Mycoplasma pneumoniae clinical isolates from 1962 to the present: a retrospective study. J Clin Microbiol 50:3620–3626. https://doi.org/10.1128/JCM.01755-12

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  4. Sun H, Xue G, Yan C, Li S, Cao L, Yuan Y et al (2013)Multiple-locus variable-number tandem-repeat analysis of mycoplasma pneumoniae Clinical Specimens and Proposal for Amendment of MLVA Nomenclature. PLoS One 8. https://doi.org/10.1371/journal.pone.0064607

    Article  CAS  Google Scholar 

  5. Touati A, Blouin Y, Sirand-Pugnet P, Renaudin H, Oishi T, Vergnaud G et al (2015) Molecular epidemiology of mycoplasma pneumoniae: genotyping using single nucleotide polymorphisms and snapshot technology. J Clin Microbiol 53:3182–3194. https://doi.org/10.1128/JCM.01156-15

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  6. Brown RJ, Holden MTG, Spiller OB, Chalker VJ (2015) Development of a multilocus sequence typing scheme for molecular typing of mycoplasma pneumoniae. J Clin Microbiol 53:3195–3203. https://doi.org/10.1128/JCM.01301-15

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  7. Wolff BJ, Thacker WL, Schwartz SB, Winchell JM (2008) Detection of macrolide resistance in Mycoplasma pneumoniae by real-time PCR and high-resolution melt analysis. Antimicrob Agents Chemother 52:3542–3549. https://doi.org/10.1128/AAC.00582-08

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  8. Chalker VJ, Pereyre S, Dumke R, Winchell J, Khosla P, Sun H et al (2015) International Mycoplasma pneumoniae typing study: interpretation of M pneumoniae multilocus variable-number tandem-repeat analysis. New Microbes New Infect 7:37–40. https://doi.org/10.1016/j.nmni.2015.05.005

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  9. Yan C, Sun H, Xue G, Zhao H, Wang L, Feng Y et al (2014) A single-tube multiple-locus variable-number tandem-repeat analysis of Mycoplasma pneumoniae clinical specimens by use of multiplex PCR-capillary electrophoresis. J Clin Microbiol 52:4168–4171. https://doi.org/10.1128/JCM.02178-14

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  10. Carriço JA, Silva-Costa C, Melo-Cristino J, Pinto FR, de Lencastre H, Almeida JS et al (2006) Illustration of a common framework for relating multiple typing methods by application to macrolide-resistant Streptococcus pyogenes. J Clin Microbiol 44:2524–2532. https://doi.org/10.1128/JCM.02536-05

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  11. Pereyre S, Touati A, Petitjean-Lecherbonnier J, Charron A, Vabret A, Bébéar C (2013) The increased incidence of Mycoplasma pneumoniae in France in 2011 was polyclonal, mainly involving M. pneumoniae type 1 strains. Clin Microbiol Infect 19:2–3. https://doi.org/10.1111/1469-0691.12107

    Article  CAS  Google Scholar 

  12. Diaz MH, Benitez AJ, Winchell JM (2015) Investigations of Mycoplasma pneumoniae infections in the United States: trends in molecular typing and macrolide resistance from 2006 to 2013. J Clin Microbiol 53:124–130. https://doi.org/10.1128/JCM.02597-14

    Article  PubMed  Google Scholar 

  13. Ho PL, Law PY, Chan BWK, Wong CW, To KKW, Chiu SS et al (2015) Emergence of macrolide-resistant mycoplasma pneumoniae in Hong Kong is linked to increasing macrolide resistance in multilocus variable-number tandem-repeat analysis type 4-5-7-2. J Clin Microbiol 53:3560–3564. https://doi.org/10.1128/JCM.01983-15

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  14. Whistler T, Sawatwong P, Diaz MH, Benitez AJ, Wolff BJ, Sapchookul P et al (2017) Molecular characterization of mycoplasma pneumoniae infections in two rural populations of Thailand from 2009 to 2012. J Clin Microbiol 55:2222–2223. https://doi.org/10.1128/JCM.00350-17

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  15. Kogoj R, Praprotnik M, Mrvič T, Korva M, Keše D (2018) Genetic diversity and macrolide resistance of Mycoplasma pneumoniae isolates from two consecutive epidemics in Slovenia. Eur J Clin Microbiol Infect Dis 37:99–107. https://doi.org/10.1007/s10096-017-3106-5

    Article  CAS  PubMed  Google Scholar 

  16. Suzuki Y, Seto J, Shimotai Y, Itagaki T, Katsushima Y, Katsushima F et al (2017)Multiple-locus variable-number tandem-repeat analysis of mycoplasma pneumoniae isolates between 2004 and 2014 in Yamagata, Japan: Change in molecular characteristics during an 11-year period. Jpn J Infect Dis 70:642–646. https://doi.org/10.7883/yoken.JJID.2017.276

    Article  CAS  PubMed  Google Scholar 

  17. Zhao F, Liu G, Cao B, Wu J, Gu Y, He L et al (2013)Multiple-locus variable-number tandem-repeat analysis of 201 mycoplasma pneumoniae isolates from Beijing, China, from 2008 to 2011. J Clin Microbiol 51:636–639. https://doi.org/10.1128/JCM.02567-12

    Article  PubMed  PubMed Central  Google Scholar 

  18. Xue G, Li M, Wang N, Zhao J, Wang B, Ren Z et al (2018) Comparison of the molecular characteristics of Mycoplasma pneumoniae from children across different regions of China. PLoS One 13:e0198557. https://doi.org/10.1371/journal.pone.0198557

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  19. Kenri T, Okazaki N, Yamazaki T, Narita M, Izumikawa K, Matsuoka M et al (2008) Genotyping analysis of Mycoplasma pneumoniae clinical strains in Japan between 1995 and 2005: type shift phenomenon of M. pneumoniae clinical strains. J. Med. Microbiol 57:469–475. https://doi.org/10.1099/jmm.0.47634-0

    Article  CAS  PubMed  Google Scholar 

  20. Nilsson AC, Björkman P, Welinder-Olsson C, Widell A, Persson K (2010) Clinical severity of Mycoplasma pneumoniae(MP) infection is associated with bacterial load in oropharyngeal secretions but not with MP genotype. BMC Infect Dis 10:39. https://doi.org/10.1186/1471-2334-10-39

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  21. Qu J, Yu X, Liu Y, Yin Y, Gu L, Cao B et al (2013) Specific multilocus variable-number tandem-repeat analysis genotypes of Mycoplasma pneumoniae are associated with diseases severity and macrolide susceptibility. PLoS One 8:8–12. https://doi.org/10.1371/journal.pone.0082174

    Article  CAS  Google Scholar 

Download references

Funding

The work was partially supported by the Russian State–funded budget project of ICBFM SB RAS # АААА-А17-117020210027-9.

Author information

Authors and Affiliations

  1. Laboratory of Pharmacogenomics, Institute of Chemical Biology and Fundamental Medicine, Lavrentjeva, 8, Novosibirsk, Russia, 630090

    Elena N. Voronina, Olga V. Mishukova, Maya A. Dymova & Maxim L. Filipenko

  2. Department of Molecular Biology, Novosibirsk State University, Pirogova, 2, Novosibirsk, Russia, 630090

    Elena N. Voronina & Maxim L. Filipenko

  3. Moscow City Pediatric G. Speransky Clinical Hospital, No. 9, Shmitovsky Proezd 29, Moscow, Russia, 123317

    Maria A. Gordukova, Elena V. Galeeva & Anatoliy A. Korsunskiy

  4. The Federal State Autonomous Educational Institution of Higher Education “The I.M. Sechenov First Moscow State Medical University” of the Ministry of Health of the Russian Federation , Pogodinskaya St. 1, Moscow, Russia, 119991

    Irina E. Turina & Anatoliy A. Korsunskiy

Authors
  1. Elena N. Voronina
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Maria A. Gordukova
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Irina E. Turina
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Olga V. Mishukova
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Maya A. Dymova
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Elena V. Galeeva
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Anatoliy A. Korsunskiy
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Maxim L. Filipenko
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Elena N. Voronina.

Ethics declarations

All procedures performed in studies involving human participants were in accordance with the ethical standards of the institutional research committee (the ethical committee under Speransky Children’s Municipal Clinical Hospital N9 January 21, 2014) and with the 1964 Helsinki declaration and its later amendments or comparable ethical standards.

Conflict of interest

The authors declare that they have no conflict of interest.

Informed consent

Informed consent was obtained from all individual participants included in the study.

Additional information

Publisher’s note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Voronina, E.N., Gordukova, M.A., Turina, I.E. et al. Molecular characterization of Mycoplasma pneumoniae infections in Moscow from 2015 to 2018. Eur J Clin Microbiol Infect Dis 39, 257–263 (2020). https://doi.org/10.1007/s10096-019-03717-6

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10096-019-03717-6

Keywords

Search

Navigation