Skip to main content
Log in

Bead-captured Mycobacterium tuberculosis for next-generation sequencing diagnosis of uncultured tuberculosis

  • Letter to the Editor
  • Published:
European Journal of Clinical Microbiology & Infectious Diseases Aims and scope Submit manuscript

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Fig. 1

References

  1. Lim C-S, Lee C-H, Chien Y-J, Wang J-Y, Lee L-N, Yu C-J et al (2010) Culture result of smear-positive sputum samples after 2 months of antituberculous treatment. Eur Respir J 35:218–220

    Article  CAS  Google Scholar 

  2. Brown AC, Bryant JM, Einer-Jensen K, Holdstock J, Houniet DT, Chan JZM et al (2015) Rapid whole-genome sequencing of Mycobacterium tuberculosis isolates directly from clinical samples. J Clin Microbiol 53:2230–2237

    Article  CAS  Google Scholar 

  3. Votintseva AA, Bradley P, Pankhurst L, del Ojo Elias C, Loose M, Nilgiriwala K et al (2017) Same-day diagnostic and surveillance data for tuberculosis via whole-genome sequencing of direct respiratory samples. J Clin Microbiol 55:1285–1298

    Article  CAS  Google Scholar 

  4. Doughty EL, Sergeant MJ, Adetifa I, Antonio M, Pallen MJ (2014) Culture-independent detection and characterisation of Mycobacterium tuberculosis and M. africanum in sputum samples using shotgun metagenomics on a benchtop sequencer. Peer J 2:e585

    Article  Google Scholar 

  5. Ghodbane R, Drancourt M (2013) Magnetic bead protocol for culturing Mycobacterium tuberculosis from sputum specimens. J Clin Microbiol 51:1578–1579

    Article  Google Scholar 

  6. Loukil A, Kirtania P, Bedotto M, Drancourt M (2018) FISHing Mycobacterium tuberculosis complex by use of a rpoB DNA probe bait. J Clin Microbiol 56:e00568–e00518

    CAS  PubMed  PubMed Central  Google Scholar 

  7. Loukil A, Darriet-Giudicelli F, Eldin C, Drancourt M (2018) Pulmonary tuberculosis conversion documented by microscopic staining for detection of dynamic, dormant, and dead mycobacteria (DDD staining). J Clin Microbiol 56:e01108–e01118

    CAS  PubMed  PubMed Central  Google Scholar 

  8. Zenteno-Cuevas R, Silva-Hernandez FX, Mendoza-Damian F, Ramirez-Hernandez MD, Vazquez-Medina K, Widrobo-Garcia L et al (2013) Characterisation of pks15/1 in clinical isolates of Mycobacterium tuberculosis from Mexico. Mem Inst Oswaldo Cruz 108:718–723

    Article  Google Scholar 

  9. Sengstake S, Bergval IL, Schuitema AR, de Beer JL, Phelan J, de Zwaan R et al (2017) Pyrazinamide resistance-conferring mutations in pncA and the transmission of multidrug resistant TB in Georgia. BMC Infect Dis 17:491

    Article  Google Scholar 

  10. Takiff HE, Feo O (2015) Clinical value of whole-genome sequencing of Mycobacterium tuberculosis. Lancet Infect Dis 15:1077–1090

    Article  CAS  Google Scholar 

Download references

Acknowledgments

J.S benefits from a Ph.D. grant from the IHU Méditerranée Infection and A.L. benefits from a Ph.D. grant from Aix-Marseille Université. We acknowledge Ayse Akkaya for her valuable technical assistance and Magdalen Lardière for reviewing English.

Funding

This work was financially supported by the IHU Méditerranée Infection.

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Michel Drancourt.

Ethics declarations

Conflict of interest

The authors declare that they have no conflict of interest.

Additional information

Publisher’s note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Saad, J., Loukil, A. & Drancourt, M. Bead-captured Mycobacterium tuberculosis for next-generation sequencing diagnosis of uncultured tuberculosis. Eur J Clin Microbiol Infect Dis 39, 205–207 (2020). https://doi.org/10.1007/s10096-019-03700-1

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10096-019-03700-1

Navigation