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Statistical optimization of culture medium for improved production of antimicrobial compound by Streptomyces rimosus AG-P1441

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Abstract

The nutritional requirements for antimicrobial activity of Streptomyces rimosus AG-P1441 were optimized using statistically-based experimental designs at a flask level. Based on a one-factor-at-a-time (OFAT) approach, glucose, corn starch and soybean meal were identified as the carbon and nitrogen sources having a significant effect on antimicrobial productivity. As a result of investigating the effect of glucose concentration, the highest antimicrobial activity was observed at 3% concentration. Response surface methodology (RSM) was then applied to optimize the growth medium components (corn starch, soybean meal, MgCl2 and glutamate). Antimicrobial productivity increased sharply when the medium consisted of 3% glucose, 3.5% corn starch, 2.5% soybean meal, 1.2 mM MgCl2 and 5.9 mM glutamate. The fermentation using optimized culture medium in a 5-L bioreactor allowed a significant increase in antimicrobial activity, evaluated by the paper disc assay, revealed a 29 mm inhibition zone diameter against Phytophthora capsici.

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Acknowledgements

This research was supported by a Grant (10045326) from the R&D Program of MOTIE/KEIT of Republic of Korea, the KRIBB Research Initiative Program, Republic of Korea and a Grant (NRF-2013M3A9A5076601) from a study on the strategies of improving the value of microbial resources funded by Ministry of Science, ICT and Future Planning of the Korea Government.

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Authors and Affiliations

  1. Industrial Biomaterial Research Center, Korea Research Institute of Bioscience and Biotechnology, 125 Gwahak-ro, Yuseong-gu, Daejeon, 34141, Korea

    Yoonjung Ju, Kwang-Hee Son, Chunzhi Jin, Byung Soon Hwang, Dong-Jin Park & Chang-Jin Kim

  2. University of Science and Technology, 217 Gajeong-ro, Yuseong-gu, Daejeon, 34113, Korea

    Chunzhi Jin & Chang-Jin Kim

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  1. Yoonjung Ju
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Correspondence to Chang-Jin Kim.

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Ju, Y., Son, KH., Jin, C. et al. Statistical optimization of culture medium for improved production of antimicrobial compound by Streptomyces rimosus AG-P1441. Food Sci Biotechnol 27, 581–590 (2018). https://doi.org/10.1007/s10068-017-0257-1

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  • DOI: https://doi.org/10.1007/s10068-017-0257-1

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