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Inhibitory effect of Zizania latifolia chloroform fraction on allergy-related mediator production in RBL-2H3 cells

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Abstract

Zizania latifolia exhibits anti-inflammatory and anti-allergic effects; however, the mechanisms behind these effects are unknown. Here the ethanol extract of Z. latifolia was partitioned using hexane, chloroform, ethyl acetate, butanol, and water. Subsequently, the anti-allergic effects of these fractions were evaluated in vitro. The results showed that the chloroform fraction of Z. latifolia inhibited the release of β-hexosaminidase and tumor necrosis factor (TNF-α) from RBL-2H3 cells stimulated with dinitrophenyl-bovine serum albumin (DNP-BSA). In addition, this fraction suppressed the expression of cyclooxygenase-2 (COX-2) and inhibited the activation of mitogen-activated protein kinases (MAPKs). The results obtained suggest that the chloroform fraction of Z. latifolia inhibited mast cell-mediated allergic inflammatory responses.

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References

  1. Kim JW, Lee JH, Hwang BY, Mun SH, Ko NY, Kim DK, Kim B, Kim HS, Kim YM, Choi WS. Morin inhibits Fyn kinase in mast cells and IgE-mediated type I hypersensitivity response in vivo. Biochem. Pharmacol. 77: 1506–1512 (2009)

    Article  CAS  Google Scholar 

  2. Itoh T, Ninomiya M, Yasuda M, Koshikawa K, Deyashiki Y, Nozawa Y, Akao Y, Koketsu M. Inhibitory effects of flavonoids isolated from Fragaria ananassa Duch on IgE-mediated degranulation in rat basophilic leukemia RBL-2H3. Bioorgan. Med. Chem. 17: 5374–5379 (2009)

    Article  CAS  Google Scholar 

  3. Marieb EN, Hoehn K. Human Anatomy and Physiology 7th ed. Benjamin Cummings, San Francisco, CA, USA. p. 659 (2007)

  4. Lagunoff D, Martin T, Read G. Agents that release histamine from mast cells. Annu. Rev. Pharmacol. 23: 331–351 (1983)

    Article  CAS  Google Scholar 

  5. Church MK, Levi-Schaffer F. The human mast cell. J. Allergy Clin. Immun. 99: 155–160 (1997)

    Article  CAS  Google Scholar 

  6. Ortega E, Schweitzer-Stenner R, Pecht I. Possible orientational constraints determine secretory signals induced by aggregation of IgE receptors on mast cells. EMBO J. 7: 4101–4109 (1988)

    CAS  Google Scholar 

  7. Funaba M, Ikeda T, Abe M. Degranulation in RBL-2H3 cells: Regulation by calmodulin pathway. Cell Biol. Int. 27: 879–885 (2003)

    Article  CAS  Google Scholar 

  8. Ikawati Z, Wahyuono S, Maeyama K. Screening of several Indonesian medicinal plants for their inhibitory effect on histamine release from RBL-2H3 cells. J. Ethnopharmacol. 75: 249–256 (2001)

    Article  CAS  Google Scholar 

  9. Nishigaki T. Mast cell degranulation and its inhibition by an anti-allergic agent tranilast. An electron microscopic study. Virchows Arch. B 55: 311–322 (1988)

    CAS  Google Scholar 

  10. Matsuda H, Morikawa T, Managi H, Yoshikawa M. Antiallergic principles from Alpinia galanga: structural requirements of phenylpropanoids for inhibition of degranulation and release of TNF-a and IL-4 in RBL-2H3 cells. Bioorgan. Med. Chem. Lett. 13: 3197–3202 (2003)

    Article  CAS  Google Scholar 

  11. Zhai C, Jiang X, Xu Y, Lorenz K. Protein and amino acid composition of Chinese and North American wild rice. LWT-Food Sci. Technol. 27: 380–383 (1994)

    Article  CAS  Google Scholar 

  12. Zhang H, Cao P, Agellon LB, Zhai C-K. Wild rice (Zizania latifolia (Griseb) Turcz) improves the serum lipid profile and antioxidant status of rats fed with a high fat/cholesterol diet. Brit. J. Nutr. 102: 1723–1727 (2009)

    Article  CAS  Google Scholar 

  13. Han S, Zhang H, Qin L, Zhai C. Effects of dietary carbohydrate replaced with wild rice (Zizania latifolia (Griseb) Turcz) on insulin resistance in rats fed with a high-fat/cholesterol diet. Nutrients 5: 552–564 (2013)

    Article  CAS  Google Scholar 

  14. Lee EJ, Whang EY, Whang K, Lee IS, Yang SA. Anti-allergic effect of Zizania latifolia Turcz extracts. Korean J. Food Sci. Technol. 41: 717–721 (2009)

    Google Scholar 

  15. Lee SS, Baek YS, Eun CS, Yu MH, Baek NI, Chung DK, Bang MH, Yang SA. Tricin derivatives as anti-inflammatory and anti-allergic constituents from the aerial part of Zizania latifolia. Biosci. Biotech. Bioch. 79: 700–706 (2015)

    Article  CAS  Google Scholar 

  16. Matsuda H, Tewtrakul S, Morikawa T, Nakamura A, Yoshikawa M. Anti-allergic principles from Thai zedoary: Structural requirements of curcuminoids for inhibition of degranulation and effect on the release of TNF-a and IL-4 in RBL-2H3 cells. Bioorgan. Med. Chem. 12: 5891–5898 (2004)

    Article  CAS  Google Scholar 

  17. Paul WE, Seder RA, Plaut M. Lymphokine and cytokine production by FceRI+ cells. Adv. Immunol. 53: 1–29 (1993)

    Article  CAS  Google Scholar 

  18. Finn DF, Walsh JJ. Twenty-first century mast cell stabilizers. Br. J. Pharmacol. 170: 23–37 (2013)

    Article  CAS  Google Scholar 

  19. Cunha-Melo JR, Gonzaga HM, Ali H, Huang FL, Huang KP, Beaven MA. Studies of protein kinase C in the rat basophilic leukemia (RBL-2H3) cell reveal that antigen-induced signals are not mimicked by the actions of phorbol myristate acetate and Ca2+ ionophore. J. Immunol. 143: 2617–2625 (1989)

    CAS  Google Scholar 

  20. Ludowyke RI, Scurr LL, McNally CM. Calcium ionophore-induced secretion from mast cells correlates with myosin light chain phosphorylation by protein kinase C. J. Immunol. 157: 5130–5138 (1996)

    CAS  Google Scholar 

  21. Hundley TR, Prasad AR, Beaven MA. Elevated levels of cyclooxygenase-2 in antigen-stimulated mast cells is associated with minimal activation of p38 mitogen-activated protein kinase. J. Immunol. 167: 1629–1636 (2001)

    Article  CAS  Google Scholar 

  22. Carey MA, Germolec DR, Langenbach R, Zeldin DC. Cyclooxygenase enzymes in allergic inflammation and asthma. Prost. Leuko. F.A. 69: 157–162 (2003)

    Article  CAS  Google Scholar 

  23. Rajakariar R, Yaqoob MM, Gilroy DW. COX-2 in inflammation and resolution. Mol. Interv. 6: 199–207 (2006)

    Article  CAS  Google Scholar 

  24. Kannan Y, Wilson MS. TEC and MAPK kinase signalling pathways in T helper (TH) cell development, TH2 differentiation and allergic asthma. J. Clin. Cell. Immunol. 12: 11–11 (2012)

    Google Scholar 

  25. Rivera J, Olivera A. Src family kinases and lipid mediators in control of allergic inflammation. Immunol. Rev. 217: 255–268 (2007)

    Article  CAS  Google Scholar 

  26. Chung MJ, Sohng JK, Choi DJ, Park YI. Inhibitory effect of phloretin and biochanin A on IgE-mediated allergic responses in rat basophilic leukemia RBL-2H3 cells. Life Sci. 93: 401–408 (2013)

    Article  Google Scholar 

  27. Di Stasi LC, Gomes JC, Vilegas W. Studies on anti-allergic constituents in the leaves and stems of Anchientia salutaris var. martiana (Violaceae). Chem. Pharm. Bull. 47: 890–3 (1999)

    Article  Google Scholar 

  28. Silva RO, Sousa FBM, Damasceno SRB, Carvalho NS, Silva VG, Oliveira FRMA, Sousa DP, Aragão KS, Barbosa ALR, Freitas RM, Medeiros JVR. Phytol, a diterpene alcohol, inhibits the inflammatory response by reducing cytokine production and oxidative stress. Fund. Clin. Pharmacol. 28: 455–464 (2014)

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. Major in Food Science and Technology, Keimyung University, Daegu, 42601, Korea

    Eun-Jung Lee, Mi-Hee Yu, Coralia V. Garcia & Seun-Ah Yang

  2. School of Science and Engineering of Chemical Materials, Kumoh National Institute of Technology, Gumi, Gyeongbuk, 39177, Korea

    Kwang-Hwan Jhee

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  1. Eun-Jung Lee
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  2. Mi-Hee Yu
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  3. Coralia V. Garcia
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  5. Seun-Ah Yang
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Correspondence to Seun-Ah Yang.

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Lee, EJ., Yu, MH., Garcia, C.V. et al. Inhibitory effect of Zizania latifolia chloroform fraction on allergy-related mediator production in RBL-2H3 cells. Food Sci Biotechnol 26, 481–487 (2017). https://doi.org/10.1007/s10068-017-0066-6

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  • DOI: https://doi.org/10.1007/s10068-017-0066-6

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