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Identification and molecular properties of SUMO-binding proteins in Arabidopsis

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Molecules and Cells

Abstract

Reversible conjugation of the small ubiquitin modifier (SUMO) peptide to proteins (SUMOylation) plays important roles in cellular processes in animals and yeasts. However, little is known about plant SUMO targets. To identify SUMO substrates in Arabidopsis and to probe for biological functions of SUMO proteins, we constructed 6xHis-3xFLAG fused AtSUMO1 (HFAtSUMO1) controlled by the CaMV35S promoter for transformation into Arabidopsis Col-0. After heat treatment, an increased sumoylation pattern was detected in the transgenic plants. SUMO1-modified proteins were selected after two-dimensional gel electrophoresis (2-DE) image analysis and identified using matrix-assisted laser-desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS). We identified 27 proteins involved in a variety of processes such as nucleic acid metabolism, signaling, metabolism, and including proteins of unknown functions. Binding and sumoylation patterns were confirmed independently. Surprisingly, MCM3 (At5G46280), a DNA replication licensing factor, only interacted with and became sumoylated by AtSUMO1, but not by SUMO1ΔGG or AtSUMO3. The results suggest specific interactions between sumoylation targets and particular sumoylation enzymes.

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Authors and Affiliations

  1. Division of Applied Life Science (Brain Korea 21 Program), and Plant Molecular Biology and Biotechnology Research Center, Gyeongsang National University, Jinju, 660-701, Korea

    Hyeong Cheol Park, Wonkyun Choi, Hee Jin Park, Mi Sun Cheong, Yoon Duck Koo, Gilok Shin, Woo Sik Chung, Woe-Yeon Kim, Ray A. Bressan, Hans J. Bohnert, Sang Yeol Lee & Dae-Jin Yun

  2. Department of Horticulture and Landscape Architecture, Purdue University, West Lafayette, Indiana, USA

    Hee Jin Park & Ray A. Bressan

  3. Bio-Crops Development Division, Department of Agricultural Biotechnology, National Academy of Agricultural Science, Rural Development Administration, Suwon, 441-707, Korea

    Min Gab Kim

  4. King Abdullah University of Science and Technology, Thuwal, 23955-6900, Saudi Arabia

    Ray A. Bressan

  5. Departments of Plant Biology and of Crop Sciences, University of Illinois at Urbana-Champaign, Urbana, USA

    Hans J. Bohnert

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  1. Hyeong Cheol Park
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  2. Wonkyun Choi
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  3. Hee Jin Park
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  4. Mi Sun Cheong
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  9. Min Gab Kim
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  12. Sang Yeol Lee
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  13. Dae-Jin Yun
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Correspondence to Hyeong Cheol Park or Dae-Jin Yun.

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These authors contributed equally to this work.

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Park, H.C., Choi, W., Park, H.J. et al. Identification and molecular properties of SUMO-binding proteins in Arabidopsis . Mol Cells 32, 143–151 (2011). https://doi.org/10.1007/s10059-011-2297-3

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  • DOI: https://doi.org/10.1007/s10059-011-2297-3

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