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Replication of fouling in vitro in hollow fiber dialyzers by albumin immobilization

  • Original Article
  • Artificial Kidney / Dialysis
  • Published:
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Abstract

For designing and evaluating the dialyzer and investigating the optimal therapeutic conditions, in vitro studies bring us many useful findings. In hemodialysis, however, the membrane fouling due to protein molecules reduces solute removal performance. Therefore, we investigated a method for replicating the fouling in dialyzers in aqueous experiments. After the albumin solution was circulated in the test circuit with a dialyzer, a glutaraldehyde solution was pumped into the dialyzer to immobilize albumin on the hollow fiber membrane. Under various immobilization conditions, the permeability of creatinine and vitamin B12 was evaluated by dialysis experiments. The creatinine clearance after immobilization of albumin was decreased, suggesting pore plugging by our fouling replication method. The glutaraldehyde crosslinked albumin molecules that adhered them to the membrane firmly. Moreover, the degree of fouling may be controlled by changing the concentration of albumin solution and the volume of glutaraldehyde solution used for immobilization. Our fouling replication method was applied to three types of polyester polymer alloy (PEPA) dialyzers and one polysulfone (PSf) dialyzer. This method enables to evaluate the permeability of various dialyzers with fouling in vitro that will be of great help in collecting data for designing dialyzers.

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Acknowledgements

The authors are grateful to Tomohito Nakano, Michiru Sasaki, Miori Haga and Kenji Kobori for their help in data collection.

Funding

This study is funded by Nikkiso Co., Ltd.

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Authors and Affiliations

  1. Department of Chemical Science and Technology, Faculty of Bioscience and Applied Chemistry, Hosei University, 3-7-2, Kajino-cho, Koganei, Tokyo, 184-8584, Japan

    Takayoshi Kiguchi, Narumi Tomisawa & Akihiro C. Yamashita

  2. Nikkiso Co., Ltd, Yebisu Garden Place Tower 22nd Floor, 20-3, Ebisu 4-Chome, Shibuya-ku, Tokyo, 150-6022, Japan

    Narumi Tomisawa

Authors
  1. Takayoshi Kiguchi
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  2. Narumi Tomisawa
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  3. Akihiro C. Yamashita
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Correspondence to Akihiro C. Yamashita.

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Conflict of interest

Akihiro C. Yamashita is serving as a consultant for Nikkiso Co., Tokyo, Japan. Narumi Tomisawa is an employee of Nikkiso Co. Takayoshi Kiguchi has no conflict of interest.

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A part of this paper has been presented at the 66th Annual Meeting of the Japanese Society for Dialysis Therapy in PACIFICO Yokohama in June 6, 2021.

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Kiguchi, T., Tomisawa, N. & Yamashita, A.C. Replication of fouling in vitro in hollow fiber dialyzers by albumin immobilization. J Artif Organs 25, 329–335 (2022). https://doi.org/10.1007/s10047-022-01318-3

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  • DOI: https://doi.org/10.1007/s10047-022-01318-3

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