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Enhancement of adherence of Helicobacter pylori to host cells by virus: possible mechanism of development of symptoms of gastric disease

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Abstract

It remains unclear why gastric disease does not develop in all cases of Helicobacter pylori infection. In this study, we analyzed whether simian virus 5 (SV5) enhanced adherence of H. pylori to adenocarcinoma epithelial cells (AGS). H. pylori in AGS (harboring SV5) and SV5-infected Vero cells, and an agglutination of H. pylori mixed with SV5 were observed by light microscopy, scanning and transmission electron microscopies. The adherent rate of H. pylori to SV5-infected Vero cells and treated with an anti-SV5 antibody was determined. H. pylori adhered to the surface of AGS cells near SV5 particles, as shown by scanning and transmission electron microscopies. The adherence of H. pylori to SV5-infected Vero cells was significantly enhanced compared with that to Vero cells. In contrast, the adherence of H. pylori to Vero cells was decreased by treatment with the anti-SV5 antibody. Agglutination of H. pylori mixed with SV5 was observed by scanning and transmission electron microscopies. Agglutination did not occur when SV5 was treated with the anti-SV5 antibody before mixing. These findings demonstrated that SV5 enhanced the adherence of H. pylori to host cells, suggesting that a persistently infected virus may be a factor enhancing the pathogenicity of H. pylori in humans.

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Acknowledgements

We thank Mr. Yoshihiko Fujioka and Mrs. Yukiko Takada of the Department of Microbiology and Infection Control, Osaka Medical College, and Mr. Rintaro Oide of the Department of Central Research Laboratory, Osaka Medical College for their technical help. We also thank Mrs. Hiromi Norimitsu for her help in the preparation of the manuscript.

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Author notes

  1. Yukimasa Ooi

    Present address: Infection Control Office, Osaka Medical College Hospital, Takatsuki, Japan

Authors and Affiliations

  1. Department of Microbiology and Infection Control, Osaka Medical College, 2-7 Daigaku-machi, Takatsuki-shi, Osaka, 569-8686, Japan

    Hong Wu, Takashi Nakano, Youichi Suzuki, Yukimasa Ooi & Kouichi Sano

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  1. Hong Wu
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  2. Takashi Nakano
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  3. Youichi Suzuki
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  4. Yukimasa Ooi
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Correspondence to Hong Wu.

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Wu, H., Nakano, T., Suzuki, Y. et al. Enhancement of adherence of Helicobacter pylori to host cells by virus: possible mechanism of development of symptoms of gastric disease. Med Mol Morphol 50, 103–111 (2017). https://doi.org/10.1007/s00795-017-0153-z

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  • DOI: https://doi.org/10.1007/s00795-017-0153-z

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