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A comparative study of biological properties of three root canal sealers

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A Correction to this article was published on 15 January 2024

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Abstract

Objectives

The aim of this study was to evaluate the effects of Hiflow with other two kinds of root canal sealers on the biological behavior of stem cells from the apical papilla (SCAP), the influence on inflammatory cytokines release and its antibacterial effects.

Materials and methods

Material extracts of Hiflow, iRoot SP, and AH Plus were prepared. Then, SCAP was incubated with extracts. The effects were evaluated by CCK-8, wound healing assay, ALP staining, alizarin red staining, and qRT-PCR. Meanwhile, polymorphonuclears (PMNs) and monocytes were isolated and treated with extracts for 4 h and 24 h respectively. Cell viability was analyzed by Annexin-V/PI double staining flow cytometry. The effects on the release of cytokines were observed by ELISA. The antibacterial effects of different sealers were tested against three kinds of bacteria found in chronic apical periodontitis.

Results

A series of results of SCAP showed that Hiflow and iRoot SP could promote cell proliferation, migration, and osteogenesis (p < 0.05). Although Hiflow was associated with greater cell apoptosis and necrosis when incubated with PMNs and monocytes (p < 0.05), it had an approximate release of anti-inflammatory cytokines with iRoot SP, which was higher than AH plus (p < 0.05). The co-culture showed that Hiflow and iRoot SP inhibited the colony formation of F. nucleatum (p < 0.05). However, both sealers had no obvious antibacterial effect on E. faecalis and P. gingivalis (p > 0.05).

Conclusions

In summary, Hiflow and iRoot SP both had positive biological stimulus on SCAP. Meanwhile, Hiflow showed a better induction on anti-inflammatory cytokines over the others. All the properties mentioned above and its antibacterial effect of F. nucleatum promise Hiflow a bright application prospect in endodontic uses.

Clinical relevance

References for clinical work to use BC Sealer Hiflow as a good biological root canal sealer.

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Data availability

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Change history

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Funding

This study was supported by the National Natural Science Foundation of China, No. 82071111 and Key Project of the Science and Technology Department of Sichuan Province, No. 2020YFSY0019.

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Authors and Affiliations

  1. State Key Laboratory of Oral Diseases & National Center for Stomatology & National Clinical Research Center for Oral Diseases, Department of Conservative Dentistry and Endodontics, West China Hospital of Stomatology, Sichuan University, Chengdu, 610041, Sichuan, China

    Yujia Yan, Yanyao Li, Yaqi Chi, Mengzhen Ji & Ling Zou

  2. Division of Endodontics, Department of Oral Biological & Medical Sciences, Faculty of Dentistry, The University of British Columbia, Vancouver, British Columbia, Canada

    Ya Shen

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  1. Yujia Yan
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Contributions

Yujia Yan was responsible for drafting the article, collecting the images and data analysis. Yanyao Li, Yaqi Chi and Mengzhen Ji were also responsible for data collection. Ya Shen and Ling Zou were responsible for the study design and supervision.

Corresponding authors

Correspondence to Ya Shen or Ling Zou.

Ethics declarations

Ethics approval and consent to participate

Ethical approval for this study was granted by the Ethics Committee of West China Hospital of Stomatology Sichuan University (approval number: WCHSIRB-D-2020–454). The informed consent obtained from study participates of extracted teeth was verbal, which was permitted by the Ethics Committee since the teeth were medical waste after extraction due to some other therapeutic purposes. Meanwhile, the informed consent obtained from study participates of peripheral venous blood was written.

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The authors declare no competing interests.

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The original version of this article was revised: This article was originally published with an incorrect affiliation citation for the corresponding author Ling Zou.

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Yan, Y., Li, Y., Chi, Y. et al. A comparative study of biological properties of three root canal sealers. Clin Oral Invest 28, 11 (2024). https://doi.org/10.1007/s00784-023-05402-7

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