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Effect of Oaz1 overexpression on goose ovarian granulosa cells

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Abstract

Ornithine decarboxylase antizyme 1 (OAZ1) regulates both polyamine biosynthesis and transport and acts as a tumor suppressor because of its functions in the regulation of cell proliferation, apoptosis and growth. However, the roles of OAZ1 in the regulation of polyamine metabolism, cell proliferation, apoptosis and hormone receptor expression in granulosa cells (GCs) from geese have not been determined. To define the roles of OAZ1 in primary GCs, we constructed and transfected an Oaz1 overexpression vector with a thymidine deletion into primary GCs. Oaz1 overexpression induced 65 and 30% decreases in the putrescine and spermidine contents, respectively, 225% increase in the spermine content and up-regulated the expression levels of genes associated with polyamine metabolism (but Oaz2). The Ccnd1, Pcna, Bax and Caspase 3 mRNA expression levels in GCs overexpressing Oaz1 were significantly higher than the expression levels in the vehicle group (p < 0.05). Additionally, a slight increase in the E2 concentration in the culture medium and enhanced Er, Fshr and Lhr expression were observed in the GCs 24 h after Oaz1 overexpression (p < 0.05). These data suggested that Oaz1 up-regulated the expression of genes related to polyamine metabolic enzymes and reproductive hormone receptors, disturbed intracellular polyamine homeostasis, and affected the transcription of genes associated to cell proliferation and apoptosis in goose primary GCs. Taken together, our results indicate that modifying polyamine metabolism by Oaz1 may interfere with the expression of genes involved in cell proliferation, apoptosis and responsiveness of ovary toward hormones in goose GCs.

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Abbreviations

AZIN:

Ornithine decarboxylase antizyme inhibitor

OAZ:

Ornithine decarboxylase antizyme

ODC:

Ornithine decarboxylase

DFMO:

α-Difluoromethylornithine

SAMDC:

S-adenosylmethionine decarboxylase

SPDS:

Spermidine synthase

SPMS:

Spermine synthase

SSAT:

Spermidine/spermine-N1-acetyltransferase

APAO:

N1-acetylpolyamine oxidase

SMO:

Spermine oxidase

GAPDH:

Glyceraldehyde-3-phosphate dehydrogenase

GC:

Granulosa cell

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Acknowledgements

This work was financially supported by Grants 31201798 from the National Natural Science Foundation of China and 20105103120003 from the Specialized Research Fund for the Doctoral Program of Higher Education.

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    Authors and Affiliations

    1. College of Animal Science and Technology, Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu, Sichuan, 611130, People’s Republic of China

      Bo Kang, Dongmei Jiang, Hui He, Rong Ma, Ziyu Chen & Zhixin Yi

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    1. Bo Kang
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    2. Dongmei Jiang
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    3. Hui He
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    4. Rong Ma
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    Correspondence to Bo Kang or Dongmei Jiang.

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    Funding

    This study was funded by Grants 31201798 from the National Natural Science Foundation of China and 20105103120003 from the Specialized Research Fund for the Doctoral Program of Higher Education.

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    Informed consent was obtained from all individual participants included in the study.

    Research involving human participants and/or animals

    This article does not contain any studies that involve human participants. All procedures performed in studies involving animals were in accordance with the ethical standards of the Animal Ethics Committee of the College of Animal Science and Technology, Sichuan Agricultural University or practice at which the studies were conducted.

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    Handling Editor: E. Agostinelli.

    B. Kang and D. Jiang contributed equally to this work.

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    Kang, B., Jiang, D., He, H. et al. Effect of Oaz1 overexpression on goose ovarian granulosa cells. Amino Acids 49, 1123–1132 (2017). https://doi.org/10.1007/s00726-017-2411-8

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