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Redox regulation of the MED28 and MED32 mediator subunits is important for development and senescence

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Abstract

Mediator is a conserved multi-protein complex that acts as a bridge between promoter-bound transcriptional regulators and RNA polymerase II. While redox signaling is important in adjusting plant metabolism and development, the involvement of Mediator in redox homeostasis and regulation only recently started to emerge. Our previous results show that the MED10a, MED28, and MED32 Mediator subunits form various types of covalent oligomers linked by intermolecular disulfide bonds in vitro. To link that with biological significance we have characterized Arabidopsis med32 and med28 mutants and found that they are affected in root development and senescence, phenotypes possibly associated to redox changes.

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Acknowledgments

This work was supported by grants from the Kemp foundation (GW, SB), Tryggers foundation (GW), the Berzelii Center (GW), the Swedish Cancer Society (SB), and the Swedish Research Council (SB).

Conflict of interest

The authors declare that they have no competing interests.

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Authors and Affiliations

  1. Department of Forest Genetics and Plant Physiology, Umeå Plant Science Center, Swedish University of Agricultural Sciences, 901 83, Umeå, Sweden

    Jehad Shaikhali, Céline Davoine & Gunnar Wingsle

  2. Department of Medical Biochemistry and Biophysics, Umeå Plant Science Center, Umeå University, SE-901 87, Umeå, Sweden

    Stefan Björklund

  3. Department of Microbiology and Plant Biology, University of Oklahoma, 770 Van Vleet Oval, Norman, OK, 73019, USA

    Céline Davoine

Authors
  1. Jehad Shaikhali
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  2. Céline Davoine
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  3. Stefan Björklund
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  4. Gunnar Wingsle
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Corresponding authors

Correspondence to Jehad Shaikhali or Gunnar Wingsle.

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Handling Editor: Bhumi Nath Tripathi

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Shaikhali, J., Davoine, C., Björklund, S. et al. Redox regulation of the MED28 and MED32 mediator subunits is important for development and senescence. Protoplasma 253, 957–963 (2016). https://doi.org/10.1007/s00709-015-0853-y

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