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Exploitation of microbes for enhancing bacoside content and reduction of Meloidogyne incognita infestation in Bacopa monnieri L

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Abstract

Despite the vast exploration of rhizospheric microbial wealth for crop yield enhancement, knowledge about the efficacy of microbial agents as biocontrol weapons against root-knot disease is scarce, especially in medicinal plants, viz., Bacopa monnieri. In the present investigation, rhizospheric microbes, viz., Bacillus megaterium, Glomus intraradices, Trichoderma harzianum ThU, and their combinations were evaluated for the management of Meloidogyne incognita (Kofoid and White) Chitwood and bacoside content enhancement in B. monnieri var CIM-Jagriti. A novel validated method Fourier transform near infrared was used for rapid estimation of total bacoside content. A significant reduction (2.75-fold) in root-knot indices was observed in the combined treatment of B. megaterium and T. harzianum ThU in comparison to untreated control plants. The same treatment also showed significant enhancement (1.40-fold) in total bacoside contents (plant active molecule) content using Fourier transform near-infrared (FT-NIR) method that analyses samples rapidly in an hour without solvent usage and provides ample scope for natural product studies.

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Acknowledgments

The authors are grateful to the Director of CSIR-CIMAP, Lucknow, India for his constant encouragement and providing necessary facilities.

Conflict of interest

The authors declare that there exists no potential conflict of interest among them.

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Authors and Affiliations

  1. Department of Microbial Technology and Nematology, CSIR-Central Institute of Medicinal and Aromatic Plants (CIMAP), P.O. CIMAP, Lucknow, 226015, India

    Rupali Gupta, Sudeep Tiwari, Shilpi K. Saikia, Virendra Shukla & Rakesh Pandey

  2. Department of Analytical Chemistry, CSIR-Central Institute of Medicinal and Aromatic Plants (CIMAP), P.O. CIMAP, Lucknow, 226015, India

    P. V. Ajay Kumar

  3. Govt. P. G. College, Satna, M.P, India

    Rashmi Singh & S. P. Singh

Authors
  1. Rupali Gupta
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  2. Sudeep Tiwari
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  3. Shilpi K. Saikia
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  4. Virendra Shukla
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  5. Rashmi Singh
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  6. S. P. Singh
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  7. P. V. Ajay Kumar
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  8. Rakesh Pandey
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Correspondence to Rakesh Pandey.

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Gupta, R., Tiwari, S., Saikia, S.K. et al. Exploitation of microbes for enhancing bacoside content and reduction of Meloidogyne incognita infestation in Bacopa monnieri L . Protoplasma 252, 53–61 (2015). https://doi.org/10.1007/s00709-014-0657-5

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  • DOI: https://doi.org/10.1007/s00709-014-0657-5

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