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Complete genome sequence of polygonatum kingianum mottle virus infecting Polygonatum kingianum Coll. et Hemsl in Yunnan, China

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Abstract

The complete genome sequence of a putative novel potyvirus, tentatively named “polygonatum kingianum mottle virus” (PKgMV; GenBank accession no. ON428226), infecting Polygonatum kingianum in China, was obtained by next-generation sequencing (NGS), reverse transcription polymerase chain reaction (RT-PCR), and rapid amplification of cDNA ends (RACE). PKgMV exhibits the typical genome organization and characteristics of members of the genus Potyvirus, with a length of 10,002 nucleotides (nt) and a large open reading frame (nt 108 to 9,746) encoding a polyprotein of 3,212 amino acids (aa) (363.68 kDa). Pairwise comparisons revealed that the PKgMV polyprotein shares 50.5–68.6% nt and 43.1–72.2% aa sequence identity with reported members of the genus Potyvirus. Moreover, phylogenetic analysis indicated that PKgMV is closely related to polygonatum kingianum virus 1 (PKgV1; accession no. MK427056). These results suggest that the PKgMV is a novel member of the genus Potyvirus of the family Potyviridae.

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Acknowledgments

The study was supported by the Major Science and Technology Program of Yunnan Province (202202AE090022), the Yunnan Seed Laboratory (202205AR070001), the National Natural Science Foundation of China (32160620, 31660508), and the Fund for Reserve Talents of Young and Middle-Aged Academic and Technical Leaders of Yunnan Province (202305AC160026).

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Authors and Affiliations

  1. Biotechnology and Germplasm Resources Research Institute, Yunnan Academy of Agricultural Sciences/Yunnan Provincial Key Lab of Agricultural Biotechnology, Kunming, Yunnan, 650205, P. R. China

    Maosen Wang, Xiaoxia Su, Fan Zhang, Tiantian Wang, Kuanyu Zheng & Zhongkai Zhang

  2. School of Agriculture, Yunnan University, Kunming, Yunnan, 650091, P. R. China

    Maosen Wang & Fan Zhang

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  1. Maosen Wang
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  2. Xiaoxia Su
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  3. Fan Zhang
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  4. Tiantian Wang
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  5. Kuanyu Zheng
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  6. Zhongkai Zhang
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Contributions

KZ and ZZ conceived and designed the experiments. MW performed research. FZ and MW analyzed data. XS and TW contributed new methods or models. MW, KZ, and ZZ wrote the paper.

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Correspondence to Kuanyu Zheng or Zhongkai Zhang.

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Communicated by Ioannis E. Tzanetakis

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Wang, M., Su, X., Zhang, F. et al. Complete genome sequence of polygonatum kingianum mottle virus infecting Polygonatum kingianum Coll. et Hemsl in Yunnan, China. Arch Virol 169, 39 (2024). https://doi.org/10.1007/s00705-024-05965-0

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