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Molecular characterization of a novel narnavirus infecting the phytopathogenic fungus Botryosphaeria dothidea

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Abstract

Here, a novel mycovirus, Botryosphaeria dothidea narnavirus 5 (BdNV5), was discovered in the plant-pathogenic fungus Botryosphaeria dothidea strain ZM210167-1. The BdNV5 genome sequence is 2,397 nucleotides (nt) in length and contains a putative open reading frame (ORF) encoding an RNA-dependent RNA polymerase (RdRp) with a molecular mass of 72.77 kDa. A BLASTp search using the RdRp amino acid (aa) sequence showed that it was most similar to the RdRp of Botryosphaeria dothidea narnavirus 4 (42.35%). In a phylogenetic tree based on RdRp aa sequences, BdNV5 clustered with members of the family Narnaviridae. BdNV5 is thus a novel member of the family Narnaviridae infecting the phytopathogenic fungus B. dothidea.

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Funding

This study was financially supported by the Open Fund of the State Key Laboratory for Biology of Plant Diseases and Insect Pests (SKLOF202103) and the Innovation and Entrepreneurship Training Program for College Students in Henan Province (202210466072).

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Author notes

  1. Xinzheng Song and Jianing Zhang contributed equally to this study.

Authors and Affiliations

  1. College of Plant Protection, Henan Agricultural University, Zhengzhou, Henan, 450002, China

    Xinzheng Song, Jianing Zhang, Qingzhou Ma, Yanfen Wang, Yashuang Guo & Meng Zhang

  2. Analytical Instrument Center, Henan Agricultural University, Zhengzhou, Henan, 450002, China

    Haiyan Wu

  3. State Key Laboratory for Biology of Plant Diseases and Insect Pests, Beijing, 100193, China

    Lihua Guo & Meng Zhang

Authors
  1. Xinzheng Song
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  2. Jianing Zhang
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  5. Yashuang Guo
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Correspondence to Haiyan Wu or Meng Zhang.

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Communicated by Robert H.A. Coutts

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Song, X., Zhang, J., Ma, Q. et al. Molecular characterization of a novel narnavirus infecting the phytopathogenic fungus Botryosphaeria dothidea. Arch Virol 169, 38 (2024). https://doi.org/10.1007/s00705-024-05964-1

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