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Genomic characterisation of a newly identified badnavirus infecting ivy (Hedera helix)

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Abstract

High-throughput sequencing (HTS) was used to investigate ringspots on ivy (Hedera helix) leaves. De novo assembly of HTS data generated from a total RNA extract from these leaves yielded a contig with sequence similarity to viruses of the genus Badnavirus, family Caulimoviridae. The complete genome sequence of this virus consists of 8,885 nucleotides and has three open reading frames (ORFs). Genome organisation and phylogenetic analysis identifies this newly identified virus as a new member of the genus Badnavirus for which we propose the name "ivy ringspot-associated virus" (IRSaV).

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References

  1. Bhat A, Hohn T, Selvarajan R (2016) Badnaviruses: the current global scenario. Viruses 8:177. https://doi.org/10.3390/v8060177

    Article  CAS  PubMed Central  Google Scholar 

  2. Diop SI, Geering ADW, Alfama-Depauw F et al (2018) Tracheophyte genomes keep track of the deep evolution of the Caulimoviridae. Sci Rep 8:1–9. https://doi.org/10.1038/s41598-017-16399-x

    Article  CAS  Google Scholar 

  3. Staginnus C, Richertpoggeler K (2006) Endogenous pararetroviruses: two-faced travelers in the plant genome. Trends Plant Sci 11:485–491. https://doi.org/10.1016/j.tplants.2006.08.008

    Article  CAS  PubMed  Google Scholar 

  4. Ruiz-García AB, Bester R, Olmos A, Maree HJ (2019) Bioinformatic tools and genome analysis of Citrus tristeza virus. In: Catara AF, Bar-Joseph M, Licciardello G (eds) Citrus tristeza virus: methods and protocols. Springer, New York, pp 163–178

    Chapter  Google Scholar 

  5. Sether DM, Melzer MJ, Borth WB, Hu JS (2012) Pineapple bacilliform CO virus: diversity, detection, distribution, and transmission. Plant Dis 96:1798–1804. https://doi.org/10.1094/PDIS-08-11-0718-RE

    Article  CAS  PubMed  Google Scholar 

  6. King AMQ, Lefkowitz E, Adams MJ, Carstens EB (2011) Virus taxonomy: ninth report of the International Committee on Taxonomy of Viruses. Elsevier, New York

  7. Lockhart BEL, Olszewski NE (1996) Schefflera ringspot virus, a widely distributed mealybug-transmitted badnavirus occurring in schefflera and aralia. Acta horticulturae. International Society for Horticultural Science (ISHS), Leuven, pp 196–203

    Google Scholar 

  8. Katoh K, Rozewicki J, Yamada KD (2019) MAFFT online service: multiple sequence alignment, interactive sequence choice and visualization. Brief Bioinform 20:1160–1166. https://doi.org/10.1093/bib/bbx108

    Article  PubMed  Google Scholar 

  9. Kumar S, Stecher G, Li M et al (2018) MEGA X: molecular evolutionary genetics analysis across computing platforms. Mol Biol Evol 35:1547–1549. https://doi.org/10.1093/molbev/msy096

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  10. Felsenstein J (1985) Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39:783–791. https://doi.org/10.1111/j.1558-5646.1985.tb00420.x

    Article  PubMed  Google Scholar 

  11. Jukes TH, Cantor CR (1969) Evolution of protein molecules. In: Mammalian protein metabolism. Elsevier, New York, pp 21–132

  12. Kitajima EW, Chagas CM, Rodrigues JCV (2003) Brevipalpus-transmitted plant virus and virus-like diseases: cytopathology and some recent cases. Exp Appl Acarol 30:135–160. https://doi.org/10.1023/B:APPA.0000006546.55305.e3

    Article  CAS  PubMed  Google Scholar 

  13. Kitajima EW, Rodrigues JCV, Freitas-Astua J (2010) An annotated list of ornamentals naturally found infected by Brevipalpus mite-transmitted viruses. Sci Agric 67:348–371. https://doi.org/10.1590/S0103-90162010000300014

    Article  Google Scholar 

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Correspondence to Hans J. Maree.

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Bester, R., Burger, J.T. & Maree, H.J. Genomic characterisation of a newly identified badnavirus infecting ivy (Hedera helix). Arch Virol 165, 1511–1514 (2020). https://doi.org/10.1007/s00705-020-04627-1

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  • DOI: https://doi.org/10.1007/s00705-020-04627-1

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