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Production of immunogenic recombinant L1 protein of bovine papillomavirus type 9 causing teat papillomatosis

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Abstract

Bovine papillomavirus type 9 (BPV9) is a causative agent of severe teat papillomatosis. Considering the lack of efficient BPV culture methods, recombinant proteins such as virus-like particles developed through genetic engineering may serve as a useful tool for developing effective vaccines against BPV infection. In this study, we successfully produced immunogenic particles composed of recombinant L1 protein of BPV9 (rBPV9-L1), using a baculovirus expression system. rBPV9-L1-immunized mice produced BPV9-specific IgG, which did not cross-react with BPV type 6, which is another causative agent of teat papillomatosis. Hence, immunogenic rBPV9-L1 is potentially applicable as a vaccine candidate for teat papillomatosis.

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Funding

This study was supported by a grant from the Project of NARO Bio-Oriented Technology Research Advancement Institution (the special scheme project on vitalizing management entities of agriculture, forestry, and fisheries).

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Authors and Affiliations

  1. National Institute of Animal Health, NARO, 3-1-5 Kannondai, Tsukuba, Ibaraki, 305-0856, Japan

    Satoko Watanabe, Tomoyuki Shibahara, Kiyohiko Andoh, Shinichi Hatama & Masaji Mase

  2. Department of Veterinary Science, Graduate School of Life and Environmental Sciences, Osaka Prefecture University, 1-58 Rinku-oraikita, Izumisano, Osaka, 598-8531, Japan

    Tomoyuki Shibahara

  3. United Graduate School of Veterinary Sciences, Gifu University, 1-1 Yanagido, Gifu, Gifu, 501-1193, Japan

    Masaji Mase

Authors
  1. Satoko Watanabe
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  2. Tomoyuki Shibahara
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  3. Kiyohiko Andoh
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  4. Shinichi Hatama
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  5. Masaji Mase
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Corresponding author

Correspondence to Satoko Watanabe.

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The authors declare that they have no conflict of interest.

Ethical approval

Animal experiments were carried out in accordance with the regulations and guidelines of the Animal Ethics Committee of the National Institute of Animal Health (Approval number 19-046).

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Handling Editor: Carolina Scagnolari.

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Watanabe, S., Shibahara, T., Andoh, K. et al. Production of immunogenic recombinant L1 protein of bovine papillomavirus type 9 causing teat papillomatosis. Arch Virol 165, 1441–1444 (2020). https://doi.org/10.1007/s00705-020-04612-8

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  • DOI: https://doi.org/10.1007/s00705-020-04612-8

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