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Efficient production of a lentiviral system for displaying Crimean-Congo hemorrhagic fever virus glycoproteins reveals a broad range of cellular susceptibility and neutralization ability

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Abstract

Crimean-Congo hemorrhagic fever (CCHF) is a tick-borne disease with a mortality rate of up to 50% in humans. To avoid safety concerns associated with the use of live virus in virus neutralization assays and to detect human serum neutralizing antibodies, we prepared lentiviral particles containing the CCHF glycoprotein (lenti-CCHFV-GP). Incorporation of the GP into the lentiviral particle was confirmed by electron microscopy and Western blotting. Lenti-CCHFV-GP was found to be able to infect a wide range of cell lines, including BHK-21, HeLa, HepG2, and AsPC-1 cells. In addition, lenti-CCHFV-GP was successfully used as an alternative to CCHFV for the detection of neutralizing antibodies. Sera collected from CCHF survivors neutralized lenti-CCHFV-GP particles in a dose-dependent manner. Our results suggest that the lenti-CCHFV-GP pseudovirus can be used as a safe tool for neutralization assays in low-containment laboratories.

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Acknowledgements

The authors wish to thank all colleagues in the Department of Arboviruses and Viral Hemorrhagic Fevers at the Pasteur Institute of Iran for cooperation and excellent technical assistance. We gratefully acknowledge Gary Kobinger for scientific comments during manuscript preparation.

Funding

This research was part of a PhD thesis supported by Tehran University of Medical Sciences (Grant no. 96-04-27-36866).

Author information

Authors and Affiliations

  1. Virology Department, School of Public Health, Tehran University of Medical Sciences, Tehran, 1417613151, Iran

    Abbas Ahmadi Vasmehjani, Ahmad Nejati & Shohreh Shahmahmoodi

  2. Research Centre for Emerging and Reemerging Infectious Diseases, Pasteur Institute of Iran, Tehran, Iran

    Mostafa Salehi-Vaziri

  3. Department of Arboviruses and Viral Hemorrhagic Fevers (National Reference Laboratory), Pasteur Institute of Iran, Tehran, Iran

    Mostafa Salehi-Vaziri & Mohammad Hassan Pouriayevali

  4. Department of Virology, Pasteur Institute of Iran, Tehran, Iran

    Kayhan Azadmanesh

  5. National Communicable Disease Control Centre, Ministry of Health and Medical Education, Tehran, Iran

    Mohammad Mahdi Gouya

  6. Department of Epidemiology and Biostatistics, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran

    Mahboubeh Parsaeian

  7. Food Microbiology Research Center, Tehran University of Medical Sciences, Tehran, Iran

    Shohreh Shahmahmoodi

Authors
  1. Abbas Ahmadi Vasmehjani
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Contributions

AAV and MSV designed and organized the study. AAV, KA, AN, MHP, MMG and MP contributed to the planning, designing and analysis of the experiments, and data collection. AAV performed the statistical analysis. AAV, SS and MSV provided materials and analysis tools. AAV, MSV, KA and SS wrote and critically revised the manuscript. All authors read and approved the final manuscript.

Corresponding author

Correspondence to Shohreh Shahmahmoodi.

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Conflict of interest

All authors declare that they have no conflict of interest.

Ethical approval

All procedures involving human patients were performed in accordance with the ethical standards of the Ethical Committee of Tehran University of Medical Science (Ethical Code No: 96-04-27-36866).

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Informed consent was obtained from all individual participants included in the study.

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Handling Editor: Hideki Ebihara.

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Vasmehjani, A.A., Salehi-Vaziri, M., Azadmanesh, K. et al. Efficient production of a lentiviral system for displaying Crimean-Congo hemorrhagic fever virus glycoproteins reveals a broad range of cellular susceptibility and neutralization ability. Arch Virol 165, 1109–1120 (2020). https://doi.org/10.1007/s00705-020-04576-9

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  • DOI: https://doi.org/10.1007/s00705-020-04576-9

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