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Characterization and genome analysis of novel Klebsiella phage Henu1 with lytic activity against clinical strains of Klebsiella pneumoniae

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Abstract

Klebsiella pneumoniae is an important human pathogen that is associated with a wide range of diseases, including pneumonia and septicemia. Because of the threat of drug-resistant K. pneumoniae to humans, especially carbapenem-resistant K. pneumoniae, which is becoming a growing threat to hospitalized patients, the potential use of phage therapy has generated considerable interest. Henu1, isolated from a sewage sample, was identified as a linear double-stranded DNA phage of 40,352 bp with 53.14% G + C content and 143-bp terminal repeats. The Henu1 genome contains 45 open reading frames, and no tRNA genes were found. K. pneumoniae clinical strains with the capsular types K-1, K-2, and K-57 could be infected by Henu1. No human-virulence-related genes or lysogen-formation gene clusters were detected in this phage genome, suggesting that Henu1 is a virulent phage in its bacterial host and is safe for humans.

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Funding

The Natural Science Foundation of Education Department of Henan Province under Grant 17A310015.

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Author notes

  1. Tieshan Teng and Qiming Li are co-first authors.

Authors and Affiliations

  1. School of Basic Medical Sciences, Institute of Biomedical Informatics, Henan University, Kaifeng, 475004, China

    Tieshan Teng, Qiming Li, Zhengguo Liu, Xianghui Li, Zizheng Liu, Hanshu Liu, Fangyan Liu, Longxiang Xie, Huijuan Wang, Lei Zhang, Dongdong Wu, Mingliang Chen, Yanzhang Li & Ailing Ji

  2. Joint National Laboratory for Antibody Drug Engineering, Henan University, Kaifeng, China

    Qiming Li

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  1. Tieshan Teng
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  14. Ailing Ji
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Correspondence to Yanzhang Li.

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This article does not contain any studies with human participants or animals performed by any of the authors.

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Teng, T., Li, Q., Liu, Z. et al. Characterization and genome analysis of novel Klebsiella phage Henu1 with lytic activity against clinical strains of Klebsiella pneumoniae. Arch Virol 164, 2389–2393 (2019). https://doi.org/10.1007/s00705-019-04321-x

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  • DOI: https://doi.org/10.1007/s00705-019-04321-x

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