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Genetic characterization of a novel G9P[23] rotavirus A strain identified in southwestern China with evidence of a reassortment event between human and porcine strains

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Abstract

Group A rotaviruses (RVAs) are important zoonotic pathogens that cause intestinal disease in humans and other mammals. In this study, the novel strain RVA/Pig/China/SC11/2017/G9P[23](SC11) was isolated from fecal samples from a pig farm in Sichuan province, southwestern China. The complete genome was found to be 18,347 bp in length with 11 segments. The genotype constellation of strain SC11 was G9-P[23]-I12-R1-C1-M1-A1-N1-T1-E1-H1, according to whole-genome sequencing analysis. The VP1, VP2, VP4, VP6, NSP1–NSP3, and NSP5 genes of RVA strain SC11 were found to be closely related to those of porcine and/or porcine-like human RVAs. Meanwhile, the VP7 and NSP4 genes of strain SC11 were closely related to genes of human RVAs. However, it was difficult to pinpoint the porcine or human origin of the VP3 gene of strain SC11 based on the available data. These results showed that SC11 originated from a natural reassortment event between human and pig RVA strains, and crossover points for recombination were identified at nucleotides (nt) 109-806 of NSP2. This is the first report of such a reassortant and recombinant RVA strain in the southwestern region of China.

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Funding

This study was funded by the National Key Research Program of China (Grant No. 2017YFD0502200), the Program of Main Livestock Standardized Breeding Technology Research and Demonstration (Grant No. 2016NYZ0052), the Public Welfare Scientific Research Institutes Basic Research Projects (Grant No. SASA2017A07), the Transformation Fund of Scientific and Technological Achievements of Scientific Research Institutes in Sichuan Province (Grant No. 2018YSZH0005), and the Special Finance of Sichuan (Grant No. SASA2014CZYX009).

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Author notes

  1. Danyu Chen and Long Zhou contributed equally to this study.

Authors and Affiliations

  1. Key Laboratory of Bio-Resource and Eco-Environment of Ministry of Education, Animal Disease Prevention and Food Safety Key Laboratory of Sichuan Province, College of Life Sciences, Sichuan University, Wangjiang road 29#, Chengdu, 610065, Sichuan, People’s Republic of China

    Danyu Chen, Long Zhou, Yiming Tian, Xuan Wu, Lan Feng, Xiping Zhang, Zhihui Liu, Shurui Pang, Hongning Wang & Xin Yang

  2. Sichuan Animal Science Academy, Sichuan Provincial Key laboratory of Animal Breeding and Genetics, Chengdu, 610066, People’s Republic of China

    Runmin Kang, Jifeng Yu & Yonggang Ye

Authors
  1. Danyu Chen
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  2. Long Zhou
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  3. Yiming Tian
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  4. Xuan Wu
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  12. Hongning Wang
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  13. Xin Yang
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Correspondence to Xin Yang.

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Chen, D., Zhou, L., Tian, Y. et al. Genetic characterization of a novel G9P[23] rotavirus A strain identified in southwestern China with evidence of a reassortment event between human and porcine strains. Arch Virol 164, 1229–1232 (2019). https://doi.org/10.1007/s00705-019-04188-y

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  • DOI: https://doi.org/10.1007/s00705-019-04188-y

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