Skip to main content

Advertisement

SpringerLink
Log in

Full-length genome sequence analysis of a Hungarian porcine reproductive and respiratory syndrome virus isolated from a pig with severe respiratory disease

  • Brief Report
  • Published:
Archives of Virology Aims and scope Submit manuscript

Abstract

Here, we report the isolation of a type 1 porcine reproductive and respiratory syndrome virus (PRRSV) strain from a clinical outbreak of severe respiratory problems and high fever. Next-generation sequencing was used to determine the complete genome sequence of the isolate (9625/2012). The virus belongs to a new branch within subtype 1, clade D, and shows the highest similarity to PRRSV Olot/1991 and to the Amervac vaccine strain. Mutation analysis of 9625/2012 revealed no evidence of recombination but did show a high proportion of amino acid substitutions in the putative neutralizing epitopes, suggesting an important role of selective immune pressure in the evolution of PRRSV 9625/2012.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2

References

  1. Meulenberg JJ, Hulst MM, de Meijer EJ, Moonen PL, den Besten A, de Kluyver EP, Wensvoort G, Moormann RJ (1993) Lelystad virus, the causative agent of porcine epidemic abortion and respiratory syndrome (PEARS), is related to LDV and EAV. Virology 192:62–72

    Article  CAS  PubMed  Google Scholar 

  2. Shi M, Lam TT, Hon CC, Murtaugh MP, Davies PR, Hui RK, Li J, Wong LT, Yip CW, Jiang JW, Leung FC (2010) Phylogeny-based evolutionary, demographical, and geographical dissection of North American type 2 porcine reproductive and respiratory syndrome viruses. J Virol 84:8700–8711

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  3. Stadejek T, Oleksiewicz MB, Potapchuk D, Podgórska K (2006) Porcine reproductive and respiratory syndrome virus strains of exceptional diversity in eastern Europe support the definition of new genetic subtypes. J Gen Virol 87:1835–1841

    Article  CAS  PubMed  Google Scholar 

  4. Tian K, Yu X, Zhao T, Feng Y, Cao Z, Wang C, Hu Y, Chen X, Hu D, Tian X et al (2007) Emergence of fatal PRRSV Variants: unparalleled outbreaks of atypical PRRS in China and molecular dissection of the unique hallmark. PLoS ONE 2:e526

    Article  PubMed Central  PubMed  Google Scholar 

  5. Karniychuk UU, Geldhof M, Vanhee M, van Doorsselaere J, Saveleva TA, Nauwynck HJ (2010) Pathogenesis and antigenic characterization of a new East European subtype 3 porcine reproductive and respiratory syndrome virus isolate. BMC Vet Res 6:30

    Article  PubMed Central  PubMed  Google Scholar 

  6. Morgan SB, Graham SP, Salguero FJ, Sánchez Cordón PJ, Mokhtar H, Rebel JM, Weesendorp E, Bodman-Smith KB, Steinbach F, Frossard JP (2013) Increased pathogenicity of European porcine reproductive and respiratory syndrome virus is associated with enhanced adaptive responses and viral clearance. Vet Microbiol 163:13–22

    Article  CAS  PubMed  Google Scholar 

  7. Beura LK, Subramaniam S, Vu HL, Kwon B, Pattnaik AK, Osorio FA (2012) Identification of amino acid residues important for anti-IFN activity of porcine reproductive and respiratory syndrome virus non-structural protein 1. Virology 433:431–439

    Article  CAS  PubMed  Google Scholar 

  8. Subramaniam S, Beura LK, Kwon B, Pattnaik AK, Osorio FA (2012) Amino acid residues in the non-structural protein 1 of porcine reproductive and respiratory syndrome virus involved in down-regulation of TNF-α expression in vitro and attenuation in vivo. Virology 432:241–249

    Article  CAS  PubMed  Google Scholar 

  9. Oleksiewicz MB, Bøtner A, Normann P (2002) Porcine B-cells recognize epitopes that are conserved between the structural proteins of American- and European-type porcine reproductive and respiratory syndrome virus. J Gen Virol 83:1407–1418

    CAS  PubMed  Google Scholar 

  10. Ostrowski M, Galeota JA, Jar AM, Platt KB, Osorio FA, Lopez OJ (2002) Identification of neutralizing and nonneutralizing epitopes in the porcine reproductive and respiratory syndrome virus GP5 ectodomain. J Virol 76:4241–4250

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  11. Díaz I, Pujols J, Ganges L, Gimeno M, Darwich L, Domingo M, Mateu E (2009) In silico prediction and ex vivo evaluation of potential T-cell epitopes in glycoproteins 4 and 5 and nucleocapsid protein of genotype-I (European) of porcine reproductive and respiratory syndrome virus. Vaccine 27:5603–5611

    Article  PubMed  Google Scholar 

  12. Balka G, Hornyák A, Bálint A, Benyeda Z, Rusvai M (2009) Development of a one-step real-time quantitative PCR assay based on primer-probe energy transfer for the detection of porcine reproductive and respiratory syndrome virus. J Virol Meth 158:41–45

    Article  CAS  Google Scholar 

  13. Balka G, Hornyák A, Bálint A, Kiss I, Kecskeméti S, Bakonyi T, Rusvai M (2008) Genetic diversity of porcine reproductive and respiratory syndrome virus strains circulating in Hungarian swine herds. Vet Microbiol 127:128–135

    Article  CAS  PubMed  Google Scholar 

  14. Manual of diagnostic tests and vaccines for terrestrial animals (2013) Office International des Epizooties (Paris). http://www.oie.int/international-standard-setting/terrestrial-manual/access-online/

  15. Stemke GW, Phan R, Young TF, Ross RF (1994) Differentiation of Mycoplasma hyopneumoniae, M. flocculare, and M. hyorhinis on the basis of amplification of a 16S rRNA gene sequence. Am J Vet Res 55:81–84

    CAS  PubMed  Google Scholar 

  16. Tamura K, Peterson D, Peterson N, Stecher G, Nei M, Kumar S (2011) MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 28:2731–2739

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  17. Lole KS, Bollinger RC, Paranjape RS, Gadkari D, Kulkarni SS, Novak NG, Ingersoll R, Sheppard HW, Ray SC (1999) Full-length human immunodeficiency virus type 1 genomes from subtype C-infected seroconverters in India, with evidence of intersubtype recombination. J Virol 73:152–160

    CAS  PubMed Central  PubMed  Google Scholar 

  18. Gupta R, Brunak S (2002) Prediction of N-glycosylation sites in human proteins. Pac Symp Biocomput 7:310–322

    Google Scholar 

  19. Vanhee M, Van Breedam W, Costers S, Geldhof M, Noppe Y, Nauwynck H (2011) Characterization of antigenic regions in the porcine reproductive and respiratory syndrome virus by the use of peptide-specific serum antibodies. Vaccine 29:4794–4804

    Article  CAS  PubMed  Google Scholar 

  20. Oleksiewicz MB, Bøtner A, Toft P, Grubbe T, Nielsen J, Kamstrup S, Storgaard T (2000) Emergence of porcine reproductive and respiratory syndrome virus deletion mutants: correlation with the porcine antibody response to a hypervariable site in the ORF 3 structural glycoprotein. Virology 267:135–140

    Article  CAS  PubMed  Google Scholar 

  21. Meulenberg JJ, van Nieuwstadt AP, van Essen-Zandbergen A, Langeveld JP (1997) Posttranslational processing and identification of a neutralization domain of the GP4 protein encoded by ORF4 of the Lelystad Virus. J Virol 71:6061–6070

    CAS  PubMed Central  PubMed  Google Scholar 

  22. Wissink EH, van Wijk HA, Kroese MV, Weiland E, Meulenberg JJ, Rottier PJ, van Rijn PA (2003) The major envelope protein, GP5, of a European porcine reproductive and respiratory syndrome virus contains a neutralization epitope in its N-terminal ectodomain. J Gen Virol 84:1535–1543

    Article  CAS  PubMed  Google Scholar 

  23. Van Breedam W, Costers S, Vanhee M, Gagnon CA, Rodríguez-Gómez IM, Geldhof M, Verbeeck M, Van Doorsselaere J, Karniychuk U, Nauwynck HJ (2011) Porcine reproductive and respiratory syndrome virus (PRRSV)-specific mAbs: supporting diagnostics and providing new insights into the antigenic properties of the virus. Vet Immunol Immunopathol 141:246–257

    Article  PubMed  Google Scholar 

  24. Prieto C, Vázquez A, Núñez JI, Alvarez E, Simarro I, Castro JM (2009) Influence of time on the genetic heterogeneity of Spanish porcine reproductive and respiratory syndrome virus isolates. Vet J 180:363–370

    Article  CAS  PubMed  Google Scholar 

  25. Chen Z, Lawson S, Sun Z, Zhou X, Guan X, Christopher-Hennings J, Nelson EA, Fang Y (2010) Identification of two auto-cleavage products of nonstructural protein 1 (nsp1) in porcine reproductive and respiratory syndrome virus infected cells: nsp1 function as interferon antagonist. Virology 398:87–97

    Article  CAS  PubMed  Google Scholar 

  26. Yan Y, Guo X, Ge X, Chen Y, Cha Z, Yang H (2007) Monoclonal antibody and porcine antisera recognized B-cell epitopes of Nsp2 protein of a Chinese strain of porcine reproductive and respiratory syndrome virus. Virus Res 126:207–215

    Article  CAS  PubMed  Google Scholar 

  27. Gauger PC, Faaberg KS, Guo B, Kappes MA, Opriessnig T (2012) Genetic and phenotypic characterization of a 2006 United States porcine reproductive and respiratory virus isolate associated with high morbidity and mortality in the field. Virus Res 163:98–107

    Article  CAS  PubMed  Google Scholar 

  28. Cancel-Tirado SM, Evans RB, Yoon KJ (2004) Monoclonal antibody analysis of porcine reproductive and respiratory syndrome virus epitopes associated with antibody-dependent enhancement and neutralization of virus infection. Vet Immunol Immunopathol 102:249–262

    Article  CAS  PubMed  Google Scholar 

  29. Costers S, Vanhee M, Van Breedam W, Van Doorsselaere J, Geldhof M, Nauwynck HJ (2010) GP4-specific neutralizing antibodies might be a driving force in PRRSV evolution. Virus Res 154:104–113

    Article  CAS  PubMed  Google Scholar 

  30. Thacker EL, Halbur PG, Ross RF, Thanawongnuwech R, Thacker BJ (1999) Mycoplasma hyopneumoniae potentiation of porcine reproductive and respiratory syndrome virus-induced pneumonia. Microbiol 37:620–627

    CAS  Google Scholar 

  31. Thanawongnuwech R, Thacker B, Halbur P, Thacker EL (2004) Increased production of proinflammatory cytokines following infection with porcine reproductive and respiratory syndrome virus and Mycoplasma hyopneumoniae. Clin Diagn Lab Immunol 11:901–908

    CAS  PubMed Central  PubMed  Google Scholar 

Download references

Acknowledgments

This paper was supported by the János Bolyai Research Scholarship of the Hungarian Academy of Sciences and by the OTKA fund (OTKA K-108607). We would like to thank Amelia Sidwell for her grammatical suggestions.

Author information

Authors and Affiliations

  1. National Food Chain Safety Office Veterinary Diagnostic Directorate, Tábornok u. 2, 1149, Budapest, Hungary

    Ádám Bálint, Sándor Kecskeméti, Ádám Dán & Levente Szeredi

  2. Department of Pathology, Faculty of Veterinary Science, Szent István University, István u. 2, 1078, Budapest, Hungary

    Gyula Balka

  3. Centre for Agricultural Research, Institute for Veterinary Medical Research, Hungarian Academy of Sciences, Hungária krt. 21, 1143, Budapest, Hungary

    Péter Horváth, Krisztián Bányai, Dániel Bartha, Ferenc Olasz & Zoltán Zádori

  4. National Food Chain Safety Office Directorate of Veterinary Medicinal Products, Szállás u. 8, 1107, Budapest, Hungary

    Attila Farsang

  5. Department of Virology, Immunobiology and Parasitology, National Veterinary Institute, SVA, Ulls väg 2B, 751 89, Uppsala, Sweden

    Sándor Belák

Authors
  1. Ádám Bálint
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Gyula Balka
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Péter Horváth
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Sándor Kecskeméti
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Ádám Dán
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Attila Farsang
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Levente Szeredi
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Krisztián Bányai
    View author publications

    You can also search for this author in PubMed Google Scholar

  9. Dániel Bartha
    View author publications

    You can also search for this author in PubMed Google Scholar

  10. Ferenc Olasz
    View author publications

    You can also search for this author in PubMed Google Scholar

  11. Sándor Belák
    View author publications

    You can also search for this author in PubMed Google Scholar

  12. Zoltán Zádori
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Ádám Bálint.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Bálint, Á., Balka, G., Horváth, P. et al. Full-length genome sequence analysis of a Hungarian porcine reproductive and respiratory syndrome virus isolated from a pig with severe respiratory disease. Arch Virol 160, 417–422 (2015). https://doi.org/10.1007/s00705-014-2265-2

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00705-014-2265-2

Keywords

Search

Navigation