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Genetic variability and evolution of broad bean wilt virus 1: role of recombination, selection and gene flow

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Abstract

Analysis of four genomic regions from 37 geographically diverse isolates of broad bean wilt virus 1 (BBWV-1) showed high genetic diversity in comparison to most plant viruses. Comparison of synonymous and nonsynonymous substitutions of the small coat protein gene (SCP) revealed negative selection for most amino acid positions. Phylogenetic analysis of SCP showed that some BBWV-1 isolates from distant geographical areas were genetically close, suggesting long-distance migration. Analysis of genetic differentiation revealed high gene flow between Spanish and Near Eastern subpopulations, which were separated from North-Central and South-Eastern European subpopulations. Finally, putative recombinant and reassortant genomes were also identified.

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Acknowledgments

This work was supported by Grants RTA04-004-C2, co-financed by FEDER and the Spanish Ministerio de Educación y Ciencia, and by grant ACOMP07-277 from the Generalitat Valenciana. The first and second authors were recipients of predoctoral fellowships from INIA and IVIA, respectively. We thank Drs. Aramburu, Červená, Falk, Kostova, Lesemann, Milne, Ochoa-Corona, Vetten and Yordanova for generously providing BBWV-1 isolates; and M. Boils and D. Comin for technical assistance.

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Authors and Affiliations

  1. Instituto Valenciano de Investigaciones Agrarias (IVIA), 46113, Moncada, Valencia, Spain

    Inmaculada Ferriol, Rosa M. Ferrer, José Guerri, Pedro Moreno & Luis Rubio

  2. Department of Plant Pathology, University of California Davis, Davis, 95616, CA, USA

    Inmaculada Ferriol

  3. Centro de Investigación y Tecnología Agroalimentaria de Aragón (CITA), 50080, Zaragoza, Spain

    Marisol Luis-Arteaga

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  1. Inmaculada Ferriol
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  2. Rosa M. Ferrer
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  3. Marisol Luis-Arteaga
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  4. José Guerri
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  5. Pedro Moreno
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  6. Luis Rubio
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Correspondence to Luis Rubio.

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Ferriol, I., Ferrer, R.M., Luis-Arteaga, M. et al. Genetic variability and evolution of broad bean wilt virus 1: role of recombination, selection and gene flow. Arch Virol 159, 779–784 (2014). https://doi.org/10.1007/s00705-013-1868-3

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  • DOI: https://doi.org/10.1007/s00705-013-1868-3

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