Abstract
A begomovirus and its associated betasatellites were amplified and sequenced from tobacco plants affected with leaf curl disease. The begomovirus was identified as a new strain of tomato leaf curl Pakistan virus (ToLCPKV), which is referred to here as ToLCPKV-India. A previously known betasatellite [tomato leaf curl Patna betasatellite (ToLCPaB)] and a new betasatellite were also found in leaf-curl-affected samples. The use of infectious clones of ToLCPKV-IN plus ToLCPaB for agroinoculation led to typical leaf curl, while ToLCPKV-IN together with the new betasatellite resulted in curling and chlorosis of leaves. Based on these disease symptoms, we propose to name the new betasatellite tobacco leaf chlorosis betasatellite (TbLChB).
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Acknowledgments
Department of Biotechnology, Government of India, is acknowledged for financial support. Thanks to Birendra Singh (Skane University Hospital, Sweden) for critical reading of the manuscript.
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Kumar, J., Singh, S.P., Kumar, A. et al. Detection and characterization of a new betasatellite: variation in disease symptoms of tomato leaf curl Pakistan virus-India due to associated betasatellite. Arch Virol 158, 257–261 (2013). https://doi.org/10.1007/s00705-012-1486-5
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DOI: https://doi.org/10.1007/s00705-012-1486-5