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Genetic variation and evolutionary analysis of broad bean wilt virus 2

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Abstract

The genetic variation and evolutionary mechanisms of broad bean wilt virus 2 (BBWV-2) were studied by nucleotide sequence analysis of four genomic regions of 30 isolates from different countries. Nucleotide diversity was high (0.198) for a plant virus. Phylogenetic and genetic structure analyses showed low population subdivision, suggesting a significant gene flow between distant geographic areas. Analysis of synonymous and nonsynonymous substitutions showed different negative selection pressures for different parts of the coding regions, but no positive selection was found. Several recombination detection methods showed that some BBWV-2 genomes might have originated from recombination or reassortment.

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Acknowledgments

This work was supported by grant AGL2005-07754, co-financed by FEDER and the Spanish Ministerio de Educación y Ciencia, and by grant ACOMP07-277 from the Generalitat Valenciana. The first and second authors were recipients of doctoral fellowships from the Instituto Valenciano de Investigaciones Agrarias (IVIA) and the Instituto Nacional de Investigación y Tecnología Agraria y Alimentaría (INIA), respectively. We would like to thank Drs. Davino, Bellardi, Lesemann and Jooste for kindly providing BBWV-2 isolates, and M. Boils for technical assistance.

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  1. Instituto Valenciano de Investigaciones Agrarias (IVIA), Moncada, 46113, Valencia, Spain

    Rosa M. Ferrer, Inmaculada Ferriol, Pedro Moreno, José Guerri & Luis Rubio

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  1. Rosa M. Ferrer
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  2. Inmaculada Ferriol
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  3. Pedro Moreno
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  4. José Guerri
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  5. Luis Rubio
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Correspondence to Luis Rubio.

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Ferrer, R.M., Ferriol, I., Moreno, P. et al. Genetic variation and evolutionary analysis of broad bean wilt virus 2. Arch Virol 156, 1445–1450 (2011). https://doi.org/10.1007/s00705-011-0990-3

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  • DOI: https://doi.org/10.1007/s00705-011-0990-3

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