Abstract
Beak and feather disease virus (BFDV), the causative agent of psittacine beak and feather disease (PBFD) infects psittaciformes worldwide. We provide an annotated sequence record of three full-length unique genomes of BFDV isolates from budgerigars (Melopsittacus undulatus) from a breeding farm in South Africa. The isolates share >99% nucleotide sequence identity with each other and ~96% nucleotide sequence identity to two recent isolates (Melopsittacus undulatus) from Thailand but only between 91.6 and 86.6% identity with all other full-length BFDV sequences. Maximum-likelihood analysis and recombination analysis suggest that the South African budgerigar BFDV isolates are unique to budgerigars, are non-recombinant in origin, and represent a new genotype of BFDV.
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Acknowledgments
This study was supported by funding provided by the South African National Research Foundation and by generous donation of blood and samples from infected birds.
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GenBank accession numbers: GQ165756, GQ165757, GQ165758.
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705_2010_589_MOESM1_ESM.doc
Genome sequence annotations of the budgerigar BFDV isolates from South Africa.Sequences either known or believed to have some role in circovirus replication andtranscription are marked together with a corresponding label on the nucleotide sequencealignments. To highlight differences between the sequences, wherever nucleotides in aparticular alignment column are identical to that of GQ165757 they are replaced with a“.” character. Gaps introduced to optimise the alignment are indicated with “-” character. (DOC 46 kb)
705_2010_589_MOESM2_ESM.doc
Annotated predicted replication associated (Rep, shown in A) and coat (CP, shown in B)protein sequences of budgerigar BFDV isolates from South Africa. Potential rollingcirclereplication motifs and interaction domains are highlighted in Rep. The potentialnuclear localization signal is highlighted on the CP sequence. Wherever amino acids in aparticular alignment column are identical to that of GQ165757 they are replaced with a“.” character. Gaps introduced to optimise the alignment are indicated with “-” character.The character “*” represents a stop codon. (DOC 29 kb)
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Varsani, A., de Villiers, G.K., Regnard, G.L. et al. A unique isolate of beak and feather disease virus isolated from budgerigars (Melopsittacus undulatus) in South Africa. Arch Virol 155, 435–439 (2010). https://doi.org/10.1007/s00705-010-0589-0
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DOI: https://doi.org/10.1007/s00705-010-0589-0