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Using zinc ion-enhanced fluorescence of sulfur quantum dots to improve the detection of the zinc(II)-binding antifungal drug clioquinol

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Abstract

A turn on–off fluorometric assay for clioquinol (CQ) is described here. It is based on modulation of the fluorescence of sulfur quantum dots (SQDs; best measured at excitation/emission wavelengths of 360/426 nm) by using the Zn2+-CQ affinity pair. Although the fluorescence enhancement effect of Zn2+ on SQDs was not obvious, a good quenching modulation effect was observed in the presence of CQ. This resulted in a linear analytical range that is increased by two orders of magnitude (from 0.024 μM to 0.24 μM, and 0.62 μM to 30 μM), with a detection limit (3 s) of 0.015 μM. The selectivity of the method is also improved.

Schematic illustration of the turn on-off fluorometric assay for for clioquinol (CQ) based on Zn2+-modulated sulfur quantum dots (SQDs).

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Acknowledgments

This work was supported by National Natural Science Foundation of China (31700876, 31700862), Natural Science Foundation of Shanxi Province (201601D021106), Basic Research Program of Shanxi Normal University (ZR1602).

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  1. School of Chemistry and Materials Science, Shanxi Normal University, Linfen, 041004, People’s Republic of China

    Jie Zhao & Zhefeng Fan

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  1. Jie Zhao
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  2. Zhefeng Fan
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Correspondence to Zhefeng Fan.

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Zhao, J., Fan, Z. Using zinc ion-enhanced fluorescence of sulfur quantum dots to improve the detection of the zinc(II)-binding antifungal drug clioquinol. Microchim Acta 187, 3 (2020). https://doi.org/10.1007/s00604-019-4020-6

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