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Aptamer based fluorometric determination of ATP by exploiting the FRET between carbon dots and graphene oxide

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Abstract

The authors describe a fluorometric aptamer based assay for adenosine triphosphate (ATP). It is based on the use of carbon dots (CDs) and graphene oxide (GO). The resultant CD-aptamer is adsorbed on the surface of GO via π-stacking and hydrophobic interaction, and the fluorescence of CD-aptamer is quenched via fluorescence resonance energy transfer (FRET) between CDs and GO. If ATP is present, it will bind to the aptamer and the CD-aptamer will be desorbed from GO. This will suppress FRET and the fluorescence of the CDs is restored. Under the optimal conditions and at typical excitation/emission wavelengths of 358/455 nm, the assay has a 80 pM detection limit and a linear range that extends from 0.10 to 5.0 nM concentrations of ATP. The method was successfully applied to the determination of ATP in yogurt samples. This method can also be conceivably applied to the detection of other analytes for which appropriate aptamers are available.

Schematic of a novel fluorometric ATP assay based on the fluorescence resonance energy transfer (FRET) between aptamer modified carbon dots (CD-aptamer) and graphene oxide (GO). CD-aptamer was used as the energy donor and molecular recognition probe, and GO acted as energy acceptor. This assay exhibits high sensitivity and selectivity with a detection limit as low as 80 pM.

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Acknowledgements

This work was financially supported by the National Natural Science Foundation of China (No. 61775099, No. 21705080), the Natural Science Foundation of Jiangsu Province (No. BK20171487, No. BK20171043), and the Natural Science Foundation of the Higher Education Institutions of Jiangsu Province (No. 17KJB150029).

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Authors and Affiliations

  1. School of pharmacy, Nanjing medical university, Nanjing, Jiangsu, 211166, People’s Republic of China

    Xia Cheng, Yao Cen, Guanhong Xu, Fangdi Wei, Menglan Shi, Xiaoman Xu, Muhammad Sohail & Qin Hu

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  1. Xia Cheng
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Correspondence to Qin Hu.

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Cheng, X., Cen, Y., Xu, G. et al. Aptamer based fluorometric determination of ATP by exploiting the FRET between carbon dots and graphene oxide. Microchim Acta 185, 144 (2018). https://doi.org/10.1007/s00604-018-2683-z

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